Fig. 5
Assessment of the in vivo antitumor efficacy of BDM. (A) Schematic diagram of in vivo treatment: Tumor-bearing C3H mice were treated for four times within 14 days. After that, mice were euthanasia at 15 days. (G1: Con, G2: Laser (L), G3: BD, G4: BDM, G5: BD + L, G6: BDM + L) (B) Images of tumors dissected from different groups. (C) The tumor size in different treatment group (n = 4 per group, two-way ANOVA with Dunnett’s multiple comparisons test was used). (D) The change of body weight in different treatment group (n = 4 per group, two-way ANOVA with Dunnett’s multiple comparisons test was used). (E) Representative thermographic images in G2, 5, 6 under 808 nm (1.5 W/cm2, 5 min) and 660 nm (150mW, 5 min) laser irradiation treatment at 4 h post i.v. injection of PBS, BD and BDM. (F) The change of temperature (Δt) in G2, 5, 6 (n = 5 per group, two-way ANOVA with Dunnett’s multiple comparisons test was used). (G) The representative immunohistochemical staining images and quantitative analysis of PCNA expressed in tumors in G1-6. Scale bar = 100 μm. (H) The IHC analysis of PCNA expression per µm2 in G1-6 (n = 3 per group, one-way ANOVA with Dunnett’s multiple comparisons test was used). (I) The representative immunohistochemical staining images and quantitative analysis of HMGB1 expressed in tumors in G1-6. Scale bar = 100 μm. (J) The IHC analysis of HMGB1 expression per µm2 in G1-6 (n = 3 per group, one-way ANOVA with Dunnett’s multiple comparisons test was used). (K) The representative immunohistochemical staining images and quantitative analysis of CRT expressed in tumors in G1-6. Scale bar: 100 μm. (L) The IHC analysis of CRT expression per µm2 in G1-6 (n = 3 per group, one-way ANOVA with Dunnett’s multiple comparisons test was used). (M) The representative immunohistochemical staining images and quantitative analysis of Caspase 3 expressed in tumors in G1-6. Scale bar = 100 μm. (N) The IHC analysis of Caspase 3 expression per µm2 in G1-6 (n = 3 per group, one-way ANOVA with Dunnett’s multiple comparisons test was used). (*, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001)