Fig. 7

The infiltration of different immune cell subsets in tumor microenvironment of SCC7 tumor-bearing C3H mice. (G1: Con, G2: Laser (L), G3: BD, G4: BDM, G5: BD + L, G6: BDM + L) (A) The gating strategy for different immune cell subsets in tumor microenvironment of SCC7 tumor-bearing C3H mice (n = 3 per group). The percentage of tumor-infiltrating (B) CD11b⁺ Gr-1⁺ MDSCs, (C) CD11b⁺ F4/80⁺ Macrophages. The MFI of tumor-infiltrating (D) CD86⁺ CD11b⁺ F4/80⁺ M1-like Macrophages and (E) CD206⁺ CD11b⁺ F4/80⁺ M2-like Macrophages, the percentage of tumor-infiltrating (F) CD11b⁺ CD11c⁺ DCs, (G) CD103⁺ CD11b⁺ CD11c⁺ Matured DCs, (H) CD45⁺ CD4⁺ T cells and (I) CD45⁺ CD8⁺ T cells were compared. The MFI of tumor-infiltrating (J) CD45⁺ CD8⁺ PD-1⁺ T cells. (n = 3 per group, one-way ANOVA with Dunnett’s multiple comparisons test was used. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001)