Fig. 4
From: Biointerfaces with ultrathin patterns for directional control of cell migration
Immunofluorescence micrographs of MC3T3-E1 cells cultured on patterned TiOx in arrowhead shapes with various arm lengths. All cells were stained to observe (a) nucleus, (b) focal adhesions (FAs), and (c) actin fibers under fluorescent imaging. (d) Cell nuclei were merged with the fluorescent images of vinculin and filament actin (Blue: nucleus, Green: vinculin, and Red: filament actin). Cells were equally divided into two regions, with leading region in direction of arrowhead tip and trailing region in opposite direction. White arrows point to FAs of MC3T3-E1 cells