Fig. 5
Intracellular uptake and subcellular localization analysis of FeS2-based NPs. (A) Subcellular localization of FeS2-based NPs on MNNG-HOS cells at 1 h post-transfection. Blue: cell nuclei were stained by Hoechst 33,342; Green: Endosomes or lysosomes stained by Lyso-Tracker Green; Red: NPs labeled with Cy3, Merge: Hoechst + Lyso-Tracker Green + Cy3, scale bar = 50/10 µm. (B) Quantitative analysis of cell uptake rates and (C) the mean fluorescence intensity (MFI) analysis in MNNG-HOS cells treated with FeS2-based NPs detected by flow cytometry. Blk: Blank. All data were expressed as the mean ± SD (n = 3. Statistics were done using one-way ANOVA with Tukey multi-comparisons. *p < 0.05, **p < 0.01, ***p < 0.001 and ns, no significance)