Fig. 5

miR-26a-5p@A-MSN-MG1 delivery system contribute to the prolonged suppression of microglia activation and neuroinflammation. A–I Protein levels of IL-1β (A–C), TNF-α (D–F), and IL-6 (G–I), in the L4–L5 dorsal spinal cord of mice were tested by ELISA at postoperative day 12, 14 and 21. N = 6 mice for each group. Data are represented as mean ± sem. J Immunofluorescent study revealed IBA1-positive microglia (green) in the spinal dorsal horn of different treatment groups mice at day 21 (Scale bar: 100 μm). The blue spots are DAPI nuclear staining. K-N The protein levels of IL-1β (K), TNF-α (L), IL-6 (M), IL-10 (N) in BV2 cell culture supernatant were tested by ELISA