Fig. 7

Knocking down of MFN2 attenuated the osteogenic differentiation of hPDLSCs promoted by the GOQDs. A Western blotting was performed to verify the most efficient MFN2 knockdown sequence 7 days after transfection. The blank group was not treated, the mock group was treated with transfection reagent, and the NC group was treated with NC-siRNA. B Relative quantification analysis of the data in (A) (n = 3). C hPDLSCs were treated with GOQDs for 7 days with or without MFN2 siRNA in osteogenic medium, and morphological changes in the mitochondrial network were observed via laser confocal microscopy (MitoTracker Green). D Mitochondrial skeleton images obtained through Fiji software. E Statistical analysis of mitochondrial length (n ≥ 3). F Statistical analysis of the mitochondrial footprint (n ≥ 3). G hPDLSCs were treated with GOQDs for 3 or 7 days with or without MFN2 siRNA in osteogenic medium, and the protein expression of MFN2, Runx2, Osterix, OCN, and Col-1 was assessed via Western blotting. H, I, J, K, L) Relative quantitative analysis of (G) (n = 3). M, N ALP staining and ALP activity (n = 4) assay of GOQD-treated hPDLSCs treated with or without MFN2 siRNA for 10 days in osteogenic medium. ns P > 0.05, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001