Fig. 4

Detection of ferroptosis and disruption of dual homeostasis within tumor cells by LFMP. A Evaluation of lipid peroxidation in EMT-6 cells under different treatments with BODIPY-C11 (scale bar = 100 µm). B Lipid peroxidation was measured by flow cytometry with BODIPY-C11 staining. C Intracellular MDA levels of EMT-6 cells under different treatment therapy. D The relative GSH levels in EMT-6 cells treated with MP at different concentrations. E The relative GSH levels in EMT-6 cells were subjected to different treatments. F Glutathione (GSH)/oxidized glutathione (GSSG) ratio in EMT-6 cells under different treatment. G Assessment of GPX4 expression under different treatments. H Enzyme activity of GPX4 in EMT-6 cells with different treatments. Data are shown mean ± SD, n = 5. *p < 0.05, **p < 0.01, ***p < 0.005