Fig. 2

A Cell uptake CLSM images of HHPI@GA NPs in Cal-27 cells after incubation at 0 h, 2 h, 4 h, and 6 h with or without HA. Scale bar: 25 μm. B Lysosome escape CLSM images of free ICG and HHPI@GA NPs after incubation at 1 h, 2 h, and 4 h and Pearson correlation coefficient (Pearson’s R) for colocalization analysis of images at 4 h. Scale bar: 25 and 50 μm. C ROS generation in vitro CLSM images after different treatments of Cal-27 cells. Scale bar: 100 μm