Fig. 3

A Cell viability of L-02, Cal-27, and 4T1 cells incubated with different concentrations of blank HHPI@GA NPs for 24 h measured by CCK-8 assay. B Cell viability of L-02, Cal-27, and 4T1 cells after 0–10 min laser irradiation. C Cell viability of Cal-27 cells after various treatments. D Apoptosis analysis of Cal-27 cells after various treatments. E, F CLSM images (E) and semi-quantitative analysis (F) of live/dead cell assays of Cal-27 cells. Scale bar: 50 μm. G, H Western blot of HSP90 expression in Cal-27 cells with different treatments (G) and corresponding quantitative analysis (HHPI@GA + L vs. other groups) (H). Data are shown as mean ± SD (n = 3) (*P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001)