Fig. 2
Antioxidant and anti-inflammatory effects of YBNs@PDA. (A, B) Cytotoxicity profiles of YBNs@PDA towards RAW264.7 and HT-29 cells for 48 h. (C) Cell viability of HT-29 treated with YBNs@PDA (50 µg/mL) for 12 h and 100 µM H2O2 for the following 12 h. (D, E) Flow cytometry analysis and CLSM images of intracellular ROS levels of RAW264.7 cells after treated with PBS, YBNs@PDA-25 µg/mL, YBNs@PDA-50 µg/mL and YBNs (50 µg/mL). (F) Relative expression levels of TNF-α, IL-1β, IL-6 and MCP-1 in LPS-induced RAW 264.7 macrophages after different treatments. Data are presented as mean ± standard deviation from a representative experiment (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, analysed by two-tailed unpaired Student’s t-test