Fig. 2

Synthesis and characterization of Bm@PT/Enz-miR26a. (A) The agarose gel electrophoresis results of different N/P ratios of CT/pEGFP. (B) Microscope images of transfection ability of PEI and CT in HeK-293T cells at different N/P ratios. Scale bar: 100 μm. (C) Statistical analysis of the number of EGFP-positive cells. n = 3, mean ± SD, ****p < 0.0001, one-way ANOVA. (D) Optimum ratio of PLGA to CT. (E) Optimum ratio of nanoparticles to BMSC membrane. (F) TEM images of PT, Bm@PT, and Bm@PT/Enz-miR26a. Scale bar: 50 nm. (G) High-Content Analysis System images of intracellular distribution. Nile/miFAM: 20 ng·mL^− 1. Scale bar: 100 μm. (H–I) Cellular uptake of nanoparticles by flow cytometry. Nile/miFAM: 20 ng·mL^− 1, n = 3, mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA. (J) High-Content Analysis System images of lysosome escape. C6: 20 ng·mL^− 1, Lysotracker red: 50 ng·mL^− 1. Scale bar: 100 μm