Fig. 5

FerOX internalization in peripheral blood lymphocytes. A PBMC collected from healthy donors and treated with DOX, FerOX and Myocet (1 μM) for 1, 3 and 24 h to assess drug uptake. Drug uptake has been monitored during time by flow cytometry measuring DOX mean fluorescence intensity in DOX⁺ cells. Statistical significance vs DOX ****p < 0.001 (Two-way ANOVA). B PBMC treated with DOX, FerOX and Myocet at 1 and 10 μM for 24 h and acquired by confocal microscopy. Nuclei were stained with DAPI (blue), while the DOX fluorescence signal was reported in red. Scale bar = 10 µm