Fig. 6

Comparison of DOX, Myocet and FerOX in vitro uptake of human peripheral T cells populations. A Representative plots (left panel) and frequencies (right panel) of DOX⁺ CD4⁺ T cells analyzed in human PBMCs after 24 h incubation with 5 µM of DOX, Myocet and FerOX. B Representative plots (left panel) and frequencies (right panel) of DOX⁺ CD8⁺ T cells analyzed in human PBMCs after 24 h incubation with 5 µM of DOX, Myocet and FerOX. Statistical significance vs DOX: *p < 0.05; **p < 0.01; ***p < 0.001 (One-way ANOVA). C Frequencies of DOX⁺ cells among Central Memory (CM), Naïve (CD197⁺ CD45RA⁺), Terminally Differentiated Effector (TDE, CD197⁻ CD45RA⁺) and Effector Memory (EM, CD197⁻ CD45RA⁻) CD4⁺ T cells. D Frequencies of DOX⁺ cells among CM, Naïve, TDE and EM CD8⁺ T cells. Statistical significance vs DOX: **p < 0.01; ***p < 0.001 (Two-way ANOVA). n = 4 / group, data are represented as mean ± SEM