Fig. 1From: DNA-based molecular classifiers for the profiling of gene expression signaturesDNA-based molecular classifier for profiling of gene expression signatures. AÂ Universal workflow for DNA-based molecular classifier to interpretation of gene expression signatures. BÂ Process of asymmetric PCR and associative strand displacement to capture relative relationship of low-concentration RNAs and convert them to general coding input. CÂ Asymmetric PCR for different concentrations of miRNAs. DÂ Plot of fluorescence at cycle 52 versus logarithm base 2 of initial miRNA concentrations. \(R^2\) = 0.9974. EÂ Native PAGE performed to analyze PCR products derived from different initial concentrations of miRNA. FÂ Fluorescence kinetics experiments for various concentrations of ssDNA are transformed into programmable inputs. GÂ Plots of Steady-state fluorescence intensity versus initial ssDNA concentrations. \(R^2\) = 0.9938. HÂ miRNAs are transformed into programmable inputs by asymmetric PCR and associative strand displacement. As expected, we observed a linear relationship between logarithm base 2 of initial miRNA concentrations and concentration of input. \(R^2\) = 0.9913Back to article page