Fig. 2

Arbitrary weight assignment to signatures with competitive inhibition system. A Scheme for DNA-based competitive inhibition system. DNA non-covalent catalysis adapted from entropy-driven reactions. Input act as a catalyst to catalyze the reaction between Amplifier and AF, resulting in the production of output for the following computation. Meanwhile, Input can also participate in Inhibitor to generate inactive products Waste with a same rate constant. B Scheme for irreversible competitive inhibition model of enzymatic reactions. when \([Input]_{0} \le [Inhibitor]_{0}\) , final concentration of Output is linear in the initial concentration of Input. C Fluorescence kinetics of different concentrations of Input (1, 2, 3 and 4 nM) with different weights (w = 2.5, 3.5, and 4.5). Amplifier = 100 nM. Inhibitor = 38.5 nM (w = 2.5), 27.5 nM (w = 3.5), 21.5 nM (w = 4.5). Experiment in scatter plot. Simulation in solid lines. D The linear relationships between the concentration of Input and Output corresponding to w = 2.5, 3.5, and 4.5. E Simulation results show different weights can be achieved by adjusting the concentration of Inhibitor (20 to 89 nM), and it was observed that the performance remained consistent across various input concentrations (1 to 6 nM). F Experimental validation for different concentrations of inhibitors, Output values correspond to different Input values. G Experimental demonstrated precise weighting of input by adjusting the concentration of Inhibitor.