Fig. 3

Implementation of mathematical operations for molecular classifier. A Scheme for a comprehensive linear classifier in silico. B Fluorescence kinetics for two-input summation systems. Amplifier = 100 nM. Inhibitor = 65.0 nM (w = 1.5), 38.5 nM (w = 2.5). Experiment in scatter plot. Simulation in solid lines. C Fluorescence kinetics for three-input summation systems. Amplifier = 100 nM. Inhibitor = 89.0 nM (w = 1), 65.0 nM (w = 1.5), 38.5 nM (w = 3.5). Experiment in scatter plot. Simulation in solid lines. D Annihilation reaction based on DNA cooperative hybridization mechanism enables comparison between the output strands. E Endpoint fluorescence measurements captured from the HEX channel for the linear classifier. F Endpoint fluorescence measurements captured from the ROX channel for the linear classifier. G Fluorescence kinetics captured from the HEX and ROX channel for the linear classifier. Blue for HEX channel. Red for ROX channel