Fig. 5

In vitro anti-oxidation and anti-inflammation of as-prepared polymers. (a) Representative fluorescent images and (b) intracellular levels of ROS measured by the fluorescence intensity of DCFH-DA of the SIRC cells after incubation with different samples for 24 h and further exposure to H₂O₂ for 24 h. (c) Representative fluorescent images of the SIRC cells and (d) intracellular levels of calcium measured by the fluorescence intensity of Fura-2, AM after incubation with different samples for 24 h and further exposure to H₂O₂ for 24 h. Levels of (e) IL-6 and (f) TNF-α from the p(MPC-co-DMA) samples (p(MPC), p(MPC₁-co-DMA₁), p(MPC₃-co-DMA₁) and p(MPC₆-co-DMA₁)). Scale bars: 100 μm. Values are mean ± SD (n = 5). *p < 0.05 vs. all groups; #p < 0.05 vs. LPS, p(MPC), p(MPC₃-co-DMA₁) and p(MPC₆-co-DMA₁) groups; ^p < 0.05 vs. Ctrl, p(MPC₁-co-DMA₁), p(MPC₃-co-DMA₁) and p(MPC₆-co-DMA₁) groups