Fig. 7
In vivo therapeutic effects of nanoformulations. (a) Corneal fluorescein staining images and (b) fluorescent staining scores of rabbit eyes at preoperation (Pre), after dry eye (DED) induction, and those with experimentally induced DED after topical administration of different formulations for h12 and 4 days. Dry eye animals receiving ATS without polymer and drug were control groups (Ctrl). *p < 0.05 vs. Ctrl group; **p < 0.005 vs. Ctrl group; ***p < 0.001 vs. Ctrl group. (c) Corneal topographic values and (d) mean K value from corneal topography of rabbit eyes after h12 and 4 days of topical instillation of different formulations. The blue and red dash lines represent the Pre and DED group values, respectively. Values are mean ± SD (n = 6). *p < 0.05 vs. all groups; +p < 0.05 vs. Pre, DED, CsA, p(MPC1-co-DMA1), p(MPC3-co-DMA1), and p(MPC6-co-DMA1) groups; #p < 0.05 vs. Pre, Ctrl, and p(MPC) groups; ^p < 0.05 vs. Pre, DED, p(MPC1-co-DMA1), p(MPC3-co-DMA1), and p(MPC6-co-DMA1) groups; $p < 0.05 vs. Pre, Ctrl, CsA, p(MPC), p(MPC3-co-DMA1), and p(MPC6-co-DMA1) groups