Fig. 9

Histological assessment for antioxidation and anti-inflammation capability. (a) Fluorescence images and (b) mean fluorescence intensity of corneal epithelium in rabbit eyes and those with experimentally induced DED 4 days after various samples administration. DED animals receiving ATS without nanomaterials and drug served as control groups (Ctrl). Green fluorescence is DCFH-DA-positive staining. Scale bars: 100 μm. (c) IL-6 immunofluorescence staining images and (d) mean fluorescence intensity of corneal epithelium in rabbit eyes and those with experimentally induced DED 4 days after various samples administration. DED animals receiving ATS without nanomaterials and drug served as control groups (Ctrl). Red fluorescence is IL-6-specific antibody staining. Scale bars: 50 μm. Values are mean ± SD (n = 6). *p < 0.05 vs. all groups; #p < 0.05 vs. Pre, DED, p(MPC₁-co-DMA₁), p(MPC₃-co-DMA₁), and p(MPC₆-co-DMA₁) groups; ^p < 0.05 vs. DED, Ctrl, CsA, p(MPC), p(MPC₃-co-DMA₁), and p(MPC₆-co-DMA₁) groups