Fig. 9

Swim bladder tissue treated using different decellularized methods: Details of different decellularized treatment methods (A); DAPI staining before and after decellularized treatment (B), where (a) is fresh swim bladder, and (b–f) correspond to groups A–E in (A), respectively; the physical structure of the tissue was observed using H&E staining before and after decellularized treatment (C); DNA test results after decellularized treatment (D); hemolysis rate after decellularized treatment (E); in vitro co-culture assay with mouse embryonic fibroblasts (MEFs) (F), in which (a, c) and (b, d) are groups E and B in image A; immunogenic assessment of three months after dural repair (G), in which a is the overall appearance of the excised material and b-d show the fibroblast distribution and inflammation at the dural repair site. Reprinted with permission from Ref. [47].