Fig. 4

Tumor targeting capability of nanoparticles in vivo. (A) Diagrammatic sketch of tumor targeted nanoparticles labelled with fluorescence through intravenous injection. (B) Biodistribution of RM-1-bearing mice in PLGA-Flu, OMVs@Flu, and hNVs@Flu groups at various time intervals by IVIS. (C) The relative fluorescence intensity of PLGA-Flu, OMVs@Flu, and hNVs@Flu groups was quantified at various time intervals (n = 3). (D) The major organs and tumors were gathered after 48 h and investigated through IVIS imaging. (E) Quantification of the relative fluorescence intensity of the tumors and major organs (n = 3). (F) Representative fluorescence images of the sections of tumors and major organs. Scale bar, 20 μm. All data are expressed as mean ± S.D. NS: no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001