Fig. 6

The hNVs@Flu-EGCG activated the immune system in vivo. (A) Images of flow cytometric analysis showing M1-like macrophages (CD80⁺) and M2-like macrophages (CD206⁺) gating on F4/80⁺CD11b⁺CD45⁺ cells within the tumor microenvironment following different therapies (n = 3). (B) Evaluation of levels of TNF-α, IL-6, and IL-10 cytokines in murine tumors post various treatments (n = 3). (C) Immunofluorescence images demonstrating infiltration of macrophages in tumors among diverse experimental cohorts. Scale bar, 100 μm. (D) Evaluation of serum PSA expression in RM-1 tumor-bearing mice following diverse treatments (n = 5). All data are expressed as mean ± S.D. NS: no significance, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001