Fig. 1

CTCs Organoid Culture Procedure and Results. (A) Workflow for CTCs isolation and culture with a pore size of 5.5 μm; (B) Growth status of CTCs; (C) Primary CTCs organoid culture at week 5; (D) CTCs organoid cell lines after passage; (E) Identification of normal-sized CTCs, diameter ≤ 20 μm, positive for DAPI and FITC, negative for CD45-PE; (F). Identification of large-sized CTCs, diameter > 20 μm, positive for DAPI and FITC, negative for CD45-PE; (G). Leukocyte identification, positive for DAPI and CD45-PE, negative for FITC; (H). CTCs culture outcomes, out of 32 collected cholangiocarcinoma samples, CTCs were successfully cultured in 20 samples, with a success rate of 62.5%; (I). Proportion statistics of normal-sized and large-sized CTCs