Fig. 4

Screening and Functional Analysis of LncRNAs. (A) Volcano plot of differentially expressed LncRNAs between M group (n = 3) and CK group (n = 3) samples. Red and blue dots represent upregulated and downregulated genes, respectively, while gray dots indicate genes without statistical significance; (B) Hierarchical clustering analysis of differentially expressed LncRNAs, with red indicating high expression and blue indicating low expression. Color gradient from blue to red represents increasing LncRNA expression levels; (C) Distribution of differentially expressed genes in the top 30 enriched GO terms across Biological Process, Cellular Component, and Molecular Function categories; (D) Bar graph showing the P-values of enriched GO terms; (E) Bar chart illustrating significantly enriched KEGG pathway annotations; (F) Scatter plot displaying enriched KEGG pathways for differentially expressed genes