Fig. 5
In vitro antioxidant capacity of HA@CUR NPs. (A) The free radical scavenging activity of HA@CUR NPs and CUR against DPPH and (B) ABTS radicals, and (C) the iron reduction ability assessment. (D, E) The capacity of equivalent concentrations of HA@CUR NPs and CUR to clear intracellular ROS as observed under laser confocal microscopy and their quantitative analysis. (F) FCM detection of ROS clearance in RAW 264.7 cells treated with H2O2 by HA@CUR NPs and CUR, and (G) the quantification of fluorescence intensity. (H) Protective effects of HA@CUR NPs and CUR on RAW264.7 cells stimulated with a solution of 1600 µM concentration of H2O2, where both increased cell viability. (I-K) HA@CUR NPs and CUR enhance the gene expression of antioxidative factors HO-1, SOD, and CAT. (n = 3) (n.s.: not significant; *p < 0.05; **p < 0.01, and ***p < 0.001)