Fig. 6

In vitro study of the effects of HA@CUR NPs and CUR on RAW 264.7 cell phenotypic transformation and gene expression of inflammatory factors under LPS stimulation. (A) FCM was used to assess the influence of HA@CUR NPs and CUR on the ratio of M1 to M2 macrophage phenotypes after LPS stimulation. CD86 (red, in the PE channel) and CD206 (red, in the PE channel) were used to specifically mark M1 and M2 type macrophages, respectively (antibody F4/80 labeled all macrophages, green, in the FITC channel). qPCR results of M1 macrophage marker gene (B) Arg1 and M2 macrophage marker gene (C) iNOS expression after RAW 264.7 cells were pre-treated with HA@CUR NPs and free CUR. (D) The gene expression levels of inflammatory factors (TNF-α, IL-1β, IL-6, Mmp8, COX-2). (n = 3) (n.s.: not significant; *p < 0.05; **p < 0.01; ***p < 0.001)