Fig. 2

High glucose decreases the efferocytosis capacity. A Schematic of Efferocytosis Assay in vitro. DiO labeled macrophages were co-cultured with Celltracker labeled Jurkat cells, followed by flow cytometry and immunofluorescence analysis. B Flow cytometry analysis of FITC-F4/80 and Celltracker double positive cells. C Representative confocal laser scanning microscopy (CLSM) images showing the localization of apoptotic cells (Jurkat cells) endocytosed by macrophages cells. Scale bar = 10 µm. D Quantitative analysis of flow cytometry data. E. Quantitative analysis of data obtained from fluorescence microscopy imaging. Data are presented as mean ± SEM of three independent experiments. Statistical significance was determined by student’s t test, ***P < 0.001