Fig. 4
Fabrication and characterization of HMNVs. A Schematic illustration for the preparation of HMNVsM−D through the hybridization of cell membrane harvested from RAW264.7 cell-HEK293T cell. B Confocal laser scanning microscopy (CLSM) images of fabricated HMNVs. RAW264.7 cell membranes were labeled with DiO (green) and HEK293T membranes were labeled with DiI (red). Scale bar = 500 µm. C Western blot analysis of MerTK and TfR expression in HMNVs. D Size distribution of HMNVs, HMNVsM, HMNVsM−D. E Zeta potential of HMNVs, HMNVsM, HMNVsM−D. F Representative TEM images of HMNVs, HMNVsM, HMNVsM−D. Scale bar = 500 nm. G Particle size change of HMNVsM−D in PBS and medium. Data are presented as mean ± SEM of three independent experiments. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. *P < 0.05