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Fig. 5 | Journal of Nanobiotechnology

Fig. 5

From: Targeted delivery of MerTK protein via cell membrane engineered nanoparticle enhances efferocytosis and attenuates atherosclerosis in diabetic ApoE−/− Mice

Fig. 5

HMNVs restores efferocytosis capacity in vitro. A Schematic of efferocytosis assay in vitro. DiO labeled macrophages were co-cultured with Celltracker labeled Jurkat cells and then underwent immunofluorescence and flow cytometry analysis. B Efferocytosis capacity were detected with flow cytometry. C Representative confocal laser scanning microscopy (CLSM) images showed the localization of apoptotic cells (Jurkat cells) endocytosed by macrophages. Scale bar = 10 µm. D Quantitative analysis of flow cytometry data. E Quantitative analysis of the fluorescence data. Data are presented as mean ± SEM of three independent experiments. Statistical significance was determined by one-way ANOVA with Tukey’s post hoc test. **P < 0.01, ***P < 0.001

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