Fig. 4
From: Mesoporous silica coated spicules for photodynamic therapy of metastatic melanoma
Safety evaluation of mSHS in vivo. a, b Study of the penetration behavior of mSHS on the skin of guinea pigs. a TEWL recovery profile of guinea pig skin following mSHS treatments was assessed over time. Values represent mean ± SD (n = 3). b Visualization of mSHS after its topical application. There were 1263 ± 382, 1033 ± 257, 558 ± 211, 33 ± 30 and 0 spicules per mm2 (n = 6) on guinea pig skin topical applied by mSHS 0, 1, 2, 5, 10 days later. And 10 days later, guinea pig skin got recovered. c The progression of micro-channels formation induced by mSHS treatment in vivo on guinea pig skin. Data presented as mean ± SD, n = 6. Scale bar was 100 µm. d, e Skin irritation and toxicty study of mSHS treatment in vivo. d: Comparison of guinea pig skin at 0 h, 24 h, 48 h, and 72 h after treatment with mSHS (10 mg per 1.77 cm2 administered with electrical massage for a duration of 2 min.) and the control group. Scale bar was 0.5 cm. e Skin cross sections with H&E stained at different time after mSHS treatment. Scale bar was 200 µm. f The quantity of cutaneous cells within the Area of Interest (AOI) at distinct temporal intervals subsequent to mSHS intervention. * indicates p < 0.05. ** indicates p < 0.01