Fig. 1

Isolation and characterization of ADNVs. (A) Flow chart of differential centrifugation procedure for ADNVs preparation. (B) Nanoparticle tracking analysis (NTA) determining the size and concentration of isolated ADNVs. (C) Transmission electron microscopy (TEM) of ADNVs. Scale bar, 200 nm. (D) Zetasizer measuring of the surface charges of ADNVs. (E) Agarose gel electrophoresis (1.2%) showing the DNA contents of ADNVs. Alternatively, ADNVs were pretreated with proteinase K (100 µg/mL), or nuclease (10 U/mL). DNA ladders as size markers. (F) The protein contents of ADNVs were detected by SDS-PAGE (10%) and Coomas Blue staining. Alternatively, ADNVs were pretreated with proteinase K (100 µg/mL), or nuclease (10 U/mL). Protein ladders as molecular weight (MW) markers. The results are one of three independent experiments. Shown are representative images