Fig. 2

ADNVs are preferentially taken by macrophages. (A) Flow cytometry of the internalization of Dil-labeled ADNVs (10, 20 or 40 µg/mL) by MH-S cells. Cells were co-incubated with Dil-labeled ADNVs at the indicated doses for 4 h. (B) Representative confocal images showing the internalization of Dil-labeled ADNVs (20 µg/mL) by MH-S cells. DAPI, nuclear. Scale bar, 100/50 µm. (C) Representative confocal images showing the phagocytosis of ADNVs (20 µg/mL) by MH-S cells upon treatment of chlorpromazine (CPZ, 10 µg/mL) or genistein (Gen, 200 µM), or vehicle respectively. Phalloidin, F-actin; Scale bar, 200/50 µm. (D) Biodistribution of Dil-labeled ADNVs when injected into mice via the caudal vein by IVIS Lumina imaging system, and the quantitative analysis. (E) Representative confocal images showing the uptake of Dil-labeled by F4/80⁺ macrophages in lung tissue slices. Scale bar, 100/10 µm. The results are one of three independent experiments (A-C). Shown are representative images, and the data are expressed as the mean ± SD (A), *P < 0.05, **P < 0.01, ***P < 0.001