Fig. 5

Administration of ADNVs alleviates endotoxin-induced lung injury in mice. (A) Simplified experimental scheme. C57BL/6 mice (n = 5) were intratracheally given LPS (1 mg/kg) for 4 h, and then treated with ADNVs (25 mg/kg, i.v.) or the vehicle (Ctrl). The mice were sacrificed 24 h later. (B) H&E staining of lung tissues and lung injury score. Scale bar, 200 μm. (C) Protein concentration, and (D) Total cell counts in BALF; (E) qPCR of the indicated cytokines in lungs; (F-H) Flow cytometry of the ratios of CD11c⁺SiglecF⁺ AMs (gated on CD45⁺CD11b⁻CD64⁺) (F), CD45⁺CD11b⁺F4/80⁺ MMs (G), and CD45⁺CD11b⁺Ly6G⁺ neutrophils (H) in BALF; (I) Flow cytometry of the percentages of dysfunctional mitochondria (Mito-Green^high/Mito-Red^low), and (J) the level of mitochondrial ROS. MFI, mean fluorescence intensity. (K) Measuring intracellular ATP levels. (L) Western-blotting of the representative mitochondrial genes. Molecular weights of the indicated molecules are depicted. Shown are representative images and the data are expressed as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001