Fig. 8

ADNVs confer the protection for mice from SARS-CoV-2 pseudovirus infection. (A) Simple flowchart of the experiment. Mice were firstly infected with hACE2-expressing adenovirus (AdV-ACE2, 2.5×10⁸ PFU/mice). 5 days later, the mice were injected with pre-made SARS-CoV-2 pseudovirus (psSARS) (5 × 10⁴ PFU/mice), followed by administration of ADNVs (25 mg/kg) or PBS (Ctrl), and 24 h later, mice were sacrificed for alveolar lavage and functional analysis (n = 5). (B) H&E staining of lung tissues and lung injury score. Scale bar, 200 μm. (C) Total cell counts, and (D) Protein concentrations in BALF. (E) qPCR assay of the indicated cytokines in lungs. (F) Confocal image showing the psSARS loads in frozen lung sections. DAPI, nucleus; GFP, psSARS. Scale bar,100 μm. Flow cytometry analysis of the ratios of (G) CD45⁺CD11b⁺F4/80⁺ macrophages, and (H) CD45⁺CD11b⁺Ly6G⁺ neutrophils in BALF. The representative images are shown and the data are expressed as the mean ± SD, *P < 0.05, **P < 0.01, ***P < 0.001