Split-Inteins for Simultaneous, site-specific conjugation of Quantum Dots to multiple protein targets In vivo
- Anna Charalambous†1,
- Ioanna Antoniades†1,
- Neophytos Christodoulou1 and
- Paris A Skourides1Email author
© Charalambous et al; licensee BioMed Central Ltd. 2011
Received: 6 April 2011
Accepted: 15 September 2011
Published: 15 September 2011
Proteins labelled with Quantum Dots (QDs) can be imaged over long periods of time with ultrahigh spatial and temporal resolution, yielding important information on the spatiotemporal dynamics of proteins within live cells or in vivo. However one of the major problems regarding the use of QDs for biological imaging is the difficulty of targeting QDs onto proteins. We have recently developed a DnaE split intein-based method to conjugate Quantum Dots (QDs) to the C-terminus of target proteins in vivo. In this study, we expand this approach to achieve site-specific conjugation of QDs to two or more proteins simultaneously with spectrally distinguishable QDs for multiparameter imaging of cellular functions.
Using the DnaE split intein we target QDs to the C-terminus of paxillin and show that paxillin-QD conjugates become localized at focal adhesions allowing imaging of the formation and dissolution of these complexes. We go on to utilize a different split intein, namely Ssp DnaB mini-intein, to demonstrate N-terminal protein tagging with QDs. Combination of these two intein systems allowed us to simultaneously target two distinct proteins with spectrally distinguishable QDs, in vivo, without any cross talk between the two intein systems.
Multiple target labeling is a unique feature of the intein based methodology which sets it apart from existing tagging methodologies in that, given the large number of characterized split inteins, the number of individual targets that can be simultaneously tagged is only limited by the number of QDs that can be spectrally distinguished within the cell. Therefore, the intein-mediated approach for simultaneous, in vivo, site-specific (N- and C-terminus) conjugation of Quantum Dots to multiple protein targets opens up new possibilities for bioimaging applications and offers an effective system to target QDs and other nanostructures to intracellular compartments as well as specific molecular complexes.
Visualizing protein localization, activity-dependent translocation and protein-protein interactions in vivo, in real time has become vital for unraveling the complexity and dynamics of biological interactions [1, 2]. Organic fluorophores have been widely used for these purposes but are subject to various limitations, most notably a lack of photostability and relatively low emission intensity, limiting study of long and short term dynamics respectively, especially when imaging takes place in vivo and in highly auto-fluorescent embryos . QDs, such as CdSe-ZnS core-shell nanoparticles, are inorganic fluorophores that circumvent these limitations due to their superior optical properties and are thus a promising alternative bioimaging tool. In contrast to organic fluorophores, QDs act as robust, broadly tunable manometers that can be excited by a single light source, offer extremely high fluorescence intensity, wide excitation spectra, narrow and tunable emission spectra, large stokes shift and resistance to photobleaching [4–9].
However QDs have a number of limitations which need to be resolved before their full potential can be realized including i) lack of versatile techniques for selective and site-specific targeting of QDs to biomolecules within specific cell compartments or within molecular complexes in vivo (ii) lack of QDs that can be targeted to biomolecules with controllable stoichiometry (iii) lack of compact QDs with small hydrodynamic diameters, close to those of biological macromolecules (iv) lack of methodologies for the efficient delivery of QDs into cells [9, 10]. Although some of the above issues are gradually being resolved, site specific targeting of QDs to proteins in vivo, still remains a major problem [11, 12]. One promising approach is based on the use of polyhistidine peptides (His-tags) fused to proteins of interest. His-tags can bind with high affinity and specificity to bivalent metal atoms such as Ni2+ or Zn2+ and can therefore efficiently assemble on the QD surface with a well-defined orientation . Another approach exploits the highly specific yet non-covalent interaction between the bacterial streptavidin protein and the small molecule vitamin biotin. QDs conjugated to streptavidin can bind with high affinity and specificity to proteins biotinylated under physiological conditions . Furthermore, the use of HaloTag proteins, which are haloalkane dehalogenase bacterial proteins that have been mutated to readily form a covalent bond with chloroalkanes has also been explored . Because chloroalkanes are very rare functional groups in biology, one can label a HaloTag fusion protein with QDs that display chloroalkane groups.
Even though these strategies afford stable QD-protein conjugates capable of withstanding complex biological environments for prolonged periods of time without significant dissociation, they are restrictive in that they do not allow labelling of different proteins simultaneously for multiparameter imaging of cellular functions. To address this challenge, we decided to take advantage of an intein-mediated ligation system. Inteins are polypeptide sequences that are able to self-excise during a process termed protein splicing, rejoining the two flanking extein sequences by a native peptide bond [16–21]. Molecular mechanisms of protein splicing have been studied and they involve N → S (or →O) acyl shift at the splice sites [18, 22, 23], formation of a branched intermediate [24, 25] and cyclization of an invariant Asn residue at the C-terminus of the intein to form succinimide , leading to excision of the intein and ligation of the exteins. Inteins have been widely used for in vitro protein semi-synthesis [20, 27], segmental isotopic labelling , QD nanosensor synthesis [29–31]in vivo protein cyclization [32, 33] and in vivo conjugation of QDs to biomolecular targets . Nearly 200 intein and intein-like sequences have been found in a wide variety of host proteins and in microorganisms belonging to bacteria, archaea and eukaryotes . Inteins share only low levels of sequence similarity but they share striking similarities in structure, reaction mechanism and evolution . It is thought that inteins first originated with just the splicing domain and then acquired the endonuclease domain, with the latter conferring genetic mobility to the intein . During intein evolution however, some inteins lost sequence continuity, such as the DnaE split intein, and as a result they exist in two fragments capable of protein trans splicing .
We go on to show that inteins can be used to target QDs to specific molecular complexes within living cells and embryos. Specifically through the targeting of QDs to the C-terminus of paxillin, we generated a full length protein-QD complex. Paxillin-QD conjugates localized efficiently to focal adhesion complexes within the cells of the developing embryo. Imaging of these complexes in real time revealed that QDs would associate with newly formed focal adhesions and would be released once the complexes were disassembled. Finally, split intein based QD conjugation may be extended to simultaneous and multiple protein tagging as long as functionally orthogonal split inteins are used, in order to prevent undesired side products due to cross-reactivity . Through the combination of the C and N terminal intein systems we were able for the first time to simultaneously target two distinct proteins with spectrally resolvable QDs in vivo. This is to our knowledge the only methodology that will allow conjugation of multiple targets with QDs without cross reactivity and should serve as an important addition to existing labeling methods.
Results and Discussion
Quantum Dots targeted to Focal Adhesion Complexes following in vivo, intein-mediated conjugation to the C-terminus of paxillin
Quantum Dots targeted to the cell membrane, following in vivo intein-mediated conjugation to the N-terminus of a membrane targeted variant of EGFP (memEGFP)
Although intein-based C-terminal conjugation of QDs to proteins is a valuable tool, it is often necessary to tag a protein at the N-terminus in order to achieve a functional conjugate. Thus, we wanted to implement an intein based strategy that would enable site-specific N-terminal conjugation in vivo, to complement the C-terminal tagging system we have already described . In addition we wanted to test a shorter synthetic peptide that would make this approach more affordable as well as easy. The value of using short synthetic intein peptides capable of trans-splicing was initially demonstrated for C-terminus-specific modifications of recombinant proteins using artificially split Npu DnaE  and Ssp GyrB inteins  and more recently for N-terminus-specific modifications using Ssp DnaB mini-intein . By taking advantage of the latter we implemented the strategy shown in Figure 1B. We went on to examine whether this approach could be used successfully for in vivo conjugation of QDs to the N-terminus of target proteins using a membrane-targeted variant of EGFP as a target. This construct, generated by the genetic fusion of the enhanced GFP to the farnesylation sequence of p21(Ras) (memEGFP) was selected due to its ability to constitutively localize to the cell membrane as it would provide clear visual confirmation of successful conjugation in the intact embryo . In addition, it is a good example of a target protein that cannot be QD-tagged at the C terminus as that would interfere with the membrane tethering function of the farnesylated residues and would lead to elimination of membrane anchoring.
In order to confirm conjugation of QDs to the N-terminus of memEGFP biochemically, we prepared lysates from injected embryos, which were run on an agarose gel, in a similar fashion to what has already been described above for the C-terminal conjugation of QDs to paxillin. As shown in Figure 2D, conjugation of QDs to the N-terminus of memEGFP was successful leading, to a higher molecular weight product, absent from the QD only lane.
Ssp DnaE and DnaB inteins do not cross splice and therefore facilitate simultaneous targeting of Quantum Dots to two different proteins in vivo
This experiment thus demonstrates that intein-mediated trans splicing facilitates simultaneous and specific tagging of two protein targets within the same embryo with spectrally resolvable QDs without cross splicing. Given the large number of orthogonal inteins it is possible that more than two targets can be simultaneously tagged with different QDs or different nanostructures.
Herein, we describe an intein-based system for conjugation of QDs to target proteins in vivo. This approach has several advantages over existing methodologies that make it truly unique, including i) site-specificity (N- or C-terminus), ii) low-intrinsic reactivity towards endogenous proteins which do not contain the intein motif required for splicing, thus eliminating mis-targeting of the QDs, iii) versatility conferred by the ability to target QDs to a single protein within any cellular compartment or molecular complex and iv) the ability to target spectrally resolvable QDs to multiple protein targets simultaneously without cross reactivity.
We have previously shown site-specific conjugation of QDs to the C-terminus of target proteins by using the naturally-split DnaE intein . However, C-terminal protein labelling with QDs can in some cases, interfere with protein localization and/or biological function, as can C-terminal fusion of fluorescent proteins [49–51]. This is due to interference with protein sorting or targeting signals located at the C-terminus of proteins, such as two common ER retrieval signals, the dilysine motif and the tetrapeptide KDEL, as well as the type 1 peroxisomal targeting signal peptide SKL . A C-terminal tag or marker could also disrupt signals for the incorporation of lipid anchors. For example, many members of the Ras superfamily carry sequences that signal the attachment of lipid anchors at their C-termini . A class of plasma membrane proteins, including cell adhesion molecules or receptors have a glycosylphosphatidylinositol (GPI) linker . The molecular signals engaging the lipid modification enzyme complexes reside at the C-terminus of these proteins and would definitely be disrupted by the addition of a fluorescent protein or QD. We therefore took advantage of the artificially split Ssp DnaB intein originally described by Sun, W. et al. , for site-specific conjugation of QDs to the N-terminus of target proteins. Ssp DnaB intein has been split artificially at a site (S1) proximal to the N- terminal, producing an N-terminal piece of only 11 aa in length and a C-terminal piece of 144 aa in length . This novel artificially split intein is quite useful due to the ease of chemical peptide synthesis and due to the fact that such short peptides are not prone to misfolding. We used the S1 split intein for site-specific conjugation of QDs to the N-terminus of a model target protein in vivo, namely mem-EGFP, and have shown that QD-memEGFP conjugates localize to the cell membrane and can be monitored in real time within the developing Xenopus embryo (Figure 5). Thus, the ability to target QDs to the N-terminus of proteins is very helpful for bioimaging studies aiming at determining protein localization and function, given that there are numerous proteins bearing C-terminal post-translational modifications or a C-terminal critical domain whose function would be impeded if a bulky QD was conjugated at the C-terminus.
We have also demonstrated, using this methodology, that Quantum Dots can be targeted via paxillin to focal adhesions, a specific molecular complex, for the first time. Focal Adhesions (FAs) are comprised of α and β integrin heterodimers that form a bridge between the intracellular actin cytoskeleton and the extracellular matrix (ECM) . While the extracellular domain of integrins binds directly to ECM proteins, the cytoplasmic tail is linked to the actin cytoskeleton via signalling and adapter proteins, such as focal adhesion kinase (FAK), vinculin, talin and paxillin . FAs play a crucial role in cell adhesion, spreading and motility by regulating various signal transduction pathways leading to rearrangement of the actin cytoskeleton [53, 54]. We have demonstrated that QDs can be efficiently targeted to focal adhesions via paxillin without altering protein localization and/or function. In fact Paxillin-QD conjugates retained full functionality as indicated by their ability to i) translocate to focal adhesions at the cell membrane (Figure 2A) and ii) associate with newly formed focal adhesion complexes and be released once the complexes were disassembled (Figure 3). This is an inherent advantage of QDs over fluorescent proteins since the former are conjugated to target proteins post-translationally and do not therefore interfere with protein folding and tertiary structure.
A useful additional application of this intein-based methodology is the simultaneous and specific conjugation of QDs to multiple proteins targets in vivo. Although fluorescent proteins already provide a straightforward solution to this problem . QD-conjugation methods are attractive complements given the superior optical properties of QDs over fluorescent proteins . Double in vivo labeling becomes possible with our system due to the existence of orthogonal pairs of split inteins that do not cross splice and therefore allow different protein targets to be simultaneously and specifically tagged with spectrally resolvable QDs within the cell. Such orthogonal split-intein combinations include Ssp DnaE and Sce VMA, Ssp DnaB and Sce VMA, Ssp DnaB and Mxe GyrA  to mention but a few and now Ssp DnaE and Ssp DnaB. In fact, given the large number of characterized split inteins, the number of individual targets that can be simultaneously tagged is only limited by the number of QDs that can be spectrally distinguished. Moreover, the fact that the trans-splicing reactions proceed with an identical molecular mechanism ensures similar reaction rates for QD-conjugation that would aid the comparison of the properties of the two proteins-otherwise the first protein of interest is already redistributing while the second protein is not yet sufficiently labelled. We have shown in this work that Ssp DnaE and Ssp DnaB inteins do not cross splice and may therefore be used to specifically target spectrally resolvable QDs to different proteins simultaneously in vivo (Figure 7).
Despite the successful conjugation of QDs to both the N and C terminus of target proteins, the current methodology and the materials used have certain limitations that need to be noted. We have observed that a pool of QDs remains in the cytosol, even when the target protein is in excess. This was expected in the case of paxillin, a cytosolic protein occasionally localized to the focal adhesion complexes on the cell membrane, but came as a surprise in the case of memEGFP, a protein expected to be exclusively localized on the cell membrane. An unwanted result of the presence of free QDs in the cytosol was the reduction of signal to noise ratio. These QDs are most likely not conjugated to the target protein due to the following two reasons. Firstly the commercially available solution of Streptavidin-coated QDs used in these experiments, contains both streptavidin-conjugated and free QDs (see Figure 6). This implies that even if the splicing reaction is 100% efficient, a portion of free QDs is still present in the cell. Secondly, in the Xenopus model, translation begins after the Midblastula Transition (~12 hours post injection). By that time, a portion of the streptavidin-conjugated QDs may have lost the streptavidin or the intein peptide (due to proteolytic degradation). This, in effect, generates additional free QDs, which will remain in the cytosol, thus reducing the apparent conjugation efficiency. Given that as the embryo develops, the amount of conjugated QDs is progressively reduced and given the target proteins' degradation rate, it is important to note that the time frame for imaging can be quite small. In addition, the presence of free QDs in the cytosol greatly impedes visualization of target proteins that do not localize to a specific organelle or structure in the cell, even early on. These limitations raise the need for i) commercially available QDs capable of retaining their conjugated biomolecule longer and ii) improved methodologies to ensure that the starting material consist of 100% conjugated QDs.
Our present results indicate efficient, covalent and site-specific in vivo-fusion of QDs to either the N- or C- terminus of a target protein within any cellular compartment or molecular complex. This methodology is notable due to its potential diagnostic and therapeutic applications, as it makes the targeting of nanostructures and nanodevices to different intracellular compartments and signalling complexes a viable possibility. Furthermore, this method is unique in that it facilitates QD conjugation to multiple target proteins, as long as orthogonal intein pairs are used. The number of potential applications for double (or multiple) in vivo labelling is quite large. Most obviously, protein localizations of two or more species can be followed simultaneously and protein-protein interactions may be explored using QDs suited for FRET experiments. In conclusion the intein-mediated approach for simultaneous, in vivo, site-specific (N- and C-terminus) conjugation of Quantum Dots to multiple protein targets, should serve as a powerful tool for bioimaging applications.
Embryos and explants
Xenopus laevis embryos from induced spawning  were staged according to Nieuwkoop and Faber (1967). Operation techniques and buffer (MMR, Ubbels, 1983) have been described . Xenopus embryos were fertilized in vitro and dejellied using 2% cysteine-HCl, pH 7.8, then maintained in 0.1× Marc's Modified Ringer's (0.1× MMR). Microinjections were performed in 4% Ficoll in 0.33× MMR. The embryos were injected with RNA and QDs conjugated to either the C-terminal part of DnaE Intein (DnaE-IC) or the N-terminal part of Ssp DnaB mini-intein (DnaB-IN) through a biotin-streptavidin bond, at the 2 and 4-cell stage according to established protocols . After injections the embryos were cultured in 4% Ficoll in 0.33× MMR until stage 8 and then cultured in 0.1× MMR at room temperature. For in vivo assays, the embryos were transferred to slides for time lapse movies using Zeiss Axiocam MR3 and the Axiovision software 4.6 to monitor GFP-QD co-localization.
Electrophoretic evaluation of streptavidin-coated QDs
Commercially available streptavidin coated QDot605 (from Invitrogen) were incubated with biotinylated DNA and non biotinylated DNA at a molar ratio of 1:100, for 30 minutes at room temperature. Following the conjugation reaction the DNA-QD mixtures were run on a 1% agarose gel to assess the percentage of QDs capable of efficient biotin-streptavidin conjugation. QDot605 were imaged using the ethidium bromide filter set on the UVP iBox Imaging System.
Chemical Synthesis of biotinylated C-terminus DnaE intein peptide (DnaE IC-Biotin) and biotinylated N-terminus DnaB mini-intein peptide (Biotin-DnaB IN)
The 47 amino acid peptide sequence of the C-terminus DnaE intein peptide (DnaE IC-Biotin):
The 11 amino acid peptide sequence of the N-terminus DnaB intein peptide (Biotin-DnaB IN):
Biotin was conjugated to a C-terminal Lysine (K) on DnaE IC via an Ahx linker (6 carbon inert linker) and to a N-terminal Cysteine (C) on DnaB IN via a three lysine linker and Ahx. Both peptides were synthesized on a 0.5 mmol scale on a 4-methylbenzhydrylamine (MBHA) resin according to the in-situ neutralization/HBTU activation protocol for Boc SPPS . In order to put a biotin at the C-terminus of DnaE intein, it was necessary to add an extra amino acid, Lys, at the C-terminus. In order to put a biotin at the N-terminus of DnaB intein, it was necessary to add three extra Lys, at the N-terminus. Lysines serve as a linking point for biotin as well as a spacer between the peptide and biotin. The DnaE IC peptide contains a cysteine protected with the NPyS group which was added as the last amino acid in the synthesis. Following chain assembly, global de-protection and cleavage from the support was achieved by treatment with HF containing 4% v/v pcresol, for 1 hour at 0°C. Following removal of the HF, the crude peptide products were precipitated and washed with anhydrous cold Et2O before being dissolved in aqueous acetonitrile (50% B) and lyophilized. The crude peptides were purified by preparative HPLC using a linear gradient of 25-45% B over 60 minutes. The purified peptides were characterized as the desired product by ESMS. The lyophilized biotinylated DnaE IC peptide was dissolved in 60% DMSO at a concentration of 1 mg/ml. The lyophilized biotinylated DnaB IN peptide was dissolved in PBS at a concentration of 1 mg/ml.
In vitro conjugation of DnaE IC-Biotin and Biotin-DnaB IN to streptavidin-coated QDs
The biotinylated peptides were diluted to 50 μM and used at 1:1 volume ratio with streptavidin-coated QDs (1 μM) (from Invitrogen or eBiosciences). To allow formation of the biotin-streptavidin bond we incubate at 24°C for 30 min. To remove any excess unbound peptide the conjugate was filtered through microcon centrifugal filter units (YM100) .
Analysis of QD-peptide conjugates
Analysis of QD-peptide conjugation was performed by electrophoresis at 60 V for 4 h at 4°C using a 0.5% agarose gel. No loading buffer was added to the samples before loading. Gels were visualized under the ethidium bromide filter (515-570 nm) with a UVP Imager (data not shown).
Alternatively analysis of QD peptide conjugation was performed by spotting nitrocellulose membranes (Whatman). Biotinylated peptides and peptides that did not contain the biotin modification were spotted on nitrocellulose membrane and blocked in PBS containing 1% BSA for 30 min at room temperature. The nitrocellulose membrane was then soaked in PBS containing streptavidin-coated QDs (1:500 dilution) for 30 min at room temperature. The membrane was washed with PBS-Tween 20 (1%) twice and visualized under the ethidium bromide filter (515-570 nm) with a UVP Imager (data not shown).
Plasmids and Cloning
All plasmids were constructed using standard molecular biology techniques and they were sequenced to verify correct coding.
A PCR fragment amplified with Fpax (5' AAATCGATATGGACGACCTCGAT 3') and Regfp (5' CCGAATTCCTTGTACAGCTCGTC 3') encoding paxillin-EGFP, using the pEGFP-N3 plasmid (from Addgene) as template was inserted into the multiple cloning site of the pCS2++ plasmid by restriction enzyme digest with ClaI-EcoRI. A PCR fragment amplified with IGpr61 (AAGGAATTCAAGTTTGCGGAATATTGCCTCAGTTTTGG) and IGpr63 (AAGCTCGAGTTATTTAATTGTCCCAGCG) encoding IN with 5 N-terminal extein residues (KFAEY), using the pJJDuet30 plasmid (from Addgene) as template was inserted at the C-terminus of Paxillin-EGFP on pCS2++ between the EcoRI-XhoI restriction sites.
The membrane targeted EGFP variant was constructed by genetically engineering a membrane targeting sequence, namely c-HaRas at the C-terminus of EGFP, via sequential PCR. Initially, a PCR fragment was encoding EGFP and half of the cHaRas membrane targeting sequence was amplified using the pEGFP-N3 plasmid (from Addgene) as template. The primers used for the first PCR were: FEGFP (5' AGCGAATTCATGGTGAGCAAGGGCGAGGAG 3') and RAEGFP-cHaras (5' gggccactctcatcaggagggttcagcttCTTGTACAGCTCGTCCATGCCG 3'). A second PCR followed using this PCR product and the following primers: FEGFP (5' AGCGAATTCATGGTGAGCAAGGGCGAGGAG 3') and RBEGFP-cHaras (5' GCCTCGAGtcaggagagcacacacttgcagctcatgcagccggggccactctc 3'). This PCR fragment encoded the fusion EGFP-cHaRas and was inserted into the multiple cloning site of the pCS2++ plasmid by restriction enzyme digest with EcoRI-XhoI. A PCR fragment amplified with FIC (ACATCGATatgttatcaccagaaatagaaaagttgtctcag) and RIC (CTGAATTCgttatggacaatgatgtcattggcgac) encoding DnaB IC using the pMAL plasmid (kind gift from Dr. Xiang-Qin Liu) as template, was inserted upstream and in frame with the EGFP-cHaRas on pCS2++ between the ClaI-EcoRI restriction sites.
All plasmids were transcribed into RNA using mMessage mMachine Sp6 kit (Ambion) and the mRNAs were purified using the Mega Clear kit (Ambion). Microinjections performed in Ficoll as mentioned above.
Electrophoretic analysis of protein trans-splicing
Biochemical analysis of protein-trans splicing was performed by lysis of injected Xenopus embryos at stage 10. Lysis was performed by pipetting up and down in the presence of proteinase inhibitors (Sigma) and DNAse (Roche). Lysates were then loaded onto agarose gels run at 100 V for 2 h, at 4°C. Gels were visualized with a UVP Imager.
Activin-induced Cell migration assays
Animal cap explants were prepared from stage 8 embryos. Cells were dissociated in CMFM (Ca2+ and Mg2+ free medium) and then treated with activin protein (1 U/ml in 1×CMFM) for 1 hour. The dissociated cells were subsequently plated in Modified Barth's Solution  into fibronectin-coated chambered coverslips (VWR). Coverslips were coated with 0.1 mg/ml fibronectin (Sigma, diluted to the appropriate concentration with MBS) for 2 hours at room temperature, and then blocked with bovine serum albumin (BSA; 50 mg/ml in MBS).
Timelapse analysis of dissociated cells was performed using a Zeiss Axiocam MR3 camera attached to a Zeiss Axiovert 135. Images were acquired and timelapse files assembled using Axiovision software 4.6.
Funding was provided by the Cyprus Research Promotion Foundation (ΑΝΑΒΑΘΜΙΣΗ/0609/28). It is acknowledged that the published research work is co-funded by the European Regional Development Fund.
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