Figure 3

Spore-surface display system in B. subtilis . (A) Schematic view of the integration of gene fusions on the chromosome. Red bars represent the gene fusion, grey and green bars represent respectively the non-essential amyE gene of B. subtilis used as integrational site and the chloramphenicol acetyl transferase (cat) gene used as a selectable marker. (B) Schematic representation of fusion proteins using either full length CotB (380 amino acids) or CotBΔ105 (275 amino acids) or CotC (66 amino acids) as carrier proteins (all in blue). The three 27-amino-acids repeats of full lenght CotB and the part of them remaining in CotBΔ105 are in black. Purple bars represent the heterologous part of the fusions (TTFC or LTB).