Figure 5

Dot blot analysis of proteins extracted from wild-type spores and from spores exposing the CotBΔ ₁₀₅ -TTFC chimera. Freshly purified spores expressing the CotBΔ₁₀₅-TTFC chimera (t0) or after 4, 8 or 12 weeks (t4, t8 and t12) of storage at room temperature were examined by dot blotting of extracted spore coat proteins. Concentrations of purified TTFC (in nanograms) and of coat proteins (in micrograms) are indicated. Proteins were loaded and reacted with monoclonal anti-TTFC antibodies (Boeringher), then with phosphatase-conjugated secondary antibodies. Signals of similar intensity were obtained with freshly purified spores and spores stored at room temperature for up to 12 weeks.