- Open Access
Plants and microbes assisted selenium nanoparticles: characterization and application
© Husen and Siddiqi; licensee BioMed Central Ltd. 2014
- Received: 15 May 2014
- Accepted: 31 July 2014
- Published: 16 August 2014
Selenium is an essential trace element and is an essential component of many enzymes without which they become inactive. The Se nanoparticles of varying shape and size may be synthesized from Se salts especially selenite and selenates in presence of reducing agents such as proteins, phenols, alcohols and amines. These biomolecules can be used to reduce Se salts in vitro but the byproducts released in the environment may be hazardous to flora and fauna. In this review, therefore, we analysed in depth, the biogenic synthesis of Se nanoparticles, their characterization and transformation into t- Se, m-Se, Se-nanoballs, Se-nanowires and Se-hollow spheres in an innocuous way preventing the environment from pollution. Their shape, size, FTIR, UV–vis, Raman spectra, SEM, TEM images and XRD pattern have been analysed. The weak forces involved in aggregation and transformation of one nano structure into the other have been carefully resolved.
- Plant extracts
Selenium was known as a notorious element until it was recognized by Schwarz and Foltz in 1957 as an essential trace element for both plants and mammals. Normally Se is available as selenate and selenite oxoanions. The reduction of soluble Se4+ and Se6+ by microbes to insoluble non toxic elemental Se is an effective way to remove it from contaminated soil, water and drainage . Se is one of the chalcogens occurring as selenate SeO4 2−, selenite SeO3 2− and selenide Se2− which may be reduced to atomic state by a precursor containing an appropriate reducing agent. Biogenic synthesis of Se nanoparticles is frequently achieved by reduction of selenate/selenite in presence of bacterial proteins or plant extracts containing phenols, flavonoids amines, alcohols, proteins and aldehydes. The deficiency of Se is known to be associated with over 40 diseases in man [2,3]. At low dosage it can stimulate the growth of the plant whereas at high dosages it can cause damage to it [4-6]. Se has also been shown to be effective against cancer [7,8]. Their compounds in the form of selenocysteine and selenomethionine are metabolized in biological system [7,8].
A variety of microorganisms, enzymes and fungi, besides plant extracts have been used to synthesise Se nanoparticles of different size and morphology. Se itself is used in rectifier, solar cells, photocopier and semiconductor. In addition, they exhibit biological activity owing to their interaction with the proteins and other biomolecules present in the bacterial cells and plant extracts, containing functional groups such as ›NH, C = O, COO and C-N . Se-nanobelts have been synthesised on large scale with an approximate diameter of 80 nm and length up to 5 μm . Se exists in many crystalline and amorphous forms but the shape, size and structure of the nanoparticles depend on the concentration, temperature, nature of biomolecules and pH of the reaction mixture. The properties of Se nanoparticles varies with size and shape for instance, Se nanospheres have high biological activity and low toxicity while Se nanowires of t-Se have high photoconductivity . Various methods have been employed to produce large scale Se-nanowires and trigonal selenium (t-Se) [12,13]. Pulse laser ablation, electro-kinetic technique, hydrothermal treatment, vapour deposition methods [10,14-16] generally used for production of Se nanoparticles on large scale require either sophisticated instruments or specific chemicals which are time consuming and uneconomical. Such methods often employ toxic chemicals or high temperature and high pressure which further pollute the environment.
In order to circumvent the effect of toxic chemicals in the fabrication of nanoparticles, biogenic protocol is generally followed [17,18]. Scientists have developed benign and harmless methods for the fabrication of nanoparticles using plant extracts, bacteria and fungi. For instance, Capsicum annum, Escherichia coli and Bacillus subtilis[19-21] have recently been used to produce nanoparticles. Over 16 different species of bacteria and Arechaea have been found to reduce colourless selenate and selenite to red elemental Se of different shape and size [22,23]. Plants and microbes act as producers and protectors of the environment when they are properly used. Pure element in its, atomic state may be produced by many bacteria [24,25] mainly due to the chemicals present in them or protein exuded by them. We have limited knowledge of the mechanism of the formation of Se nanoparticles by microbes and plant extract, nevertheless for a better understanding attempts are being made to explore the chemical reactions occurring in these media. Many bacterial strains have been found to reduce selenate/selenite to Se nanoparticles in different environment  even in sewage and sludge under both aerobic and anaerobic conditions [27-29]. It has been suggested that substantial quantity of soluble toxic selenate/selenite is reduced by bacterial strain to produce non toxic insoluble Se nanoparticles, although in doing so many such microbes would die. The production of Se0/Te0 by two anaerobic bacteria Sulfurospirillum barnesii and Bacillus selenireducens has been demonstrated by Oremland et al. and Baesman et al. [25,24].
The main objective of this review is to identify the plant extracts and bacterial strains involved in the biosynthesis of Se nanoparticles. Also the characterization and identification of Se-nanoballs, nanorods, nanowires and hollow spheres have been undertaken with a view to update the nanobiotechnology of Se nanoparticles and their application in diverse areas.
Se nanoparticles from plants, characterization and application
There is a fine line between optimum limit/or deficiency and excess of Se in living system which may cause toxicity. It is known that the Se nanoparticles prepared from biological material are less toxic than the bulk Se nanoparticles prepared from chemicals. The biomolecules present in the extract act both as reducing agent and stabilizers of Se nanoparticles. Bacteria, algae, dry fruits and plant extracts are used to produce nanoparticles. Green synthesis of selenium nanoparticles from selenious acid was achieved by dried extract of raisin (Vitis vinifera) . Like other biological materials, raisin also contains sugar, flavonoids and phenols in addition to minerals such as iron, potassium and calcium [19,31]. A change from colourless to deeply brick-red colour indicated the formation of nanoparticles. The formation of Se-nanoballs was examined at different interval of time. It took nearly 6 min to start conversion of Se ion to Se nanoparticles which was indicated by a decrease in Se ion concentration in the solution. The nature of nanoparticles was analysed by TEM images. It showed that the diameter of nanoballs ranges between 3 and 18 nm. They were found to be encapsulated with a thin polymorphic layer. The formation of Se nanoparticles was confirmed from the energy dispersive x-ray spectroscopy. The Se nanoballs were identified from their characteristic absorption peaks at 1.37KeV, 11.22KeV and 12.49KeV . The morphology of Se nanoparticles can be analysed by x-ray diffraction (XRD) analysis. The broad diffraction peak suggests the presence of amorphous nature of Se nanoparticles . Their particle size  has been found to be of the order of 12 nm.
SEM and TEM images of the α-Se nanoparticles showed that they consist of nanorods and nanoballs laced with C. annum protein which makes them slightly irregular in shape. The length and diameter of rods and nanoballs range between 1–3 μm and 0.4 μm, respectively. A closer look at the highly magnified field emission scanning electron microscopy (FESEM) image suggests that rod like nanoparticles are actually aggregates of spherical particles with protein coating, making the surface rough and uneven. It is quite likely that proteins are held together by hydrogen bonding and Se nanoparticles are held by van der Waals forces.
When the pH of the reaction mixture is lowered to 2 the time to produce α-Se nanoparticles increases. It has been observed from their FESEM images that a variety of polygonal Se nanoparticles are produced with size varying from 200–500 nm. It is of interest to note that some hollow spherical particles were also produced with a pore diameter of 160 nm. Li et al.  have hypothesized that hollow spheres are formed as a consequence of rise in temperature when the reaction product is placed in an electric field. Although, the melting point of α-Se nanoparticles is not very low (~490 K) even then this temperature is seldom achieved in such system, so that it may melt and produce hollow spheres. It is to be noted that even if microwave energy is supplied without rise in temperature only the outer surface of α-Se nanoballs, made of protein layer would melt, because organic materials have inherently lower melting point than metalloid Se. However, if these α-Se particles also melt with electronic impact even then the hollow sphere would not be produced because the lattice would rupture resulting in the formation of irregular sheets and dot like structures. It is more likely, that hollow spheres of α-Se are also formed along with solid nanoballs and polygonal structures during the synthesis of nanoparticles.
A comparison of FTIR spectrum of pure C. annum extract with the reaction mixture (C. annum extract + SeO3 2−) showed many peaks at 1652, 1542 and 1241 cm−1 corresponding to amide I, II and III bands owing to ʋ(C = O), ʋ(N-H) and ʋ(C-N) respectively . These bands slightly shift after the formation of nanoparticles. The UV–vis spectrum of the C. annum protein (washed with SDS-PAGE gel) with molecular weight of 30 kDa, showed peak (210 nm) corresponding to peptide bonds and amino residues (280 nm). As these are reducing agents they help in the formation of nanoparticles. It has also been confirmed from cyclic voltammogram that the redox reaction occurs between - 0.7 and 0.9 V .
Inorganic Se (selenite or selenate) also occur as selenomethionine, selenocysteine, selenocystathione, methyl selenol, dimethyl selenide and selenium methyl selenocysteine. Absorption of Se depends on its morphology and solubility in aqueous medium. Sodium salts of Se are generally soluble in water. One form may change into another to suit the basic requirements of binding to certain functional groups such as proteins. Different plants absorb Se in different quantities for instance; wheat accumulates Se proportional to its availability in the soil while Astragalus grown in the same soil had manifold excess of the element in it. Broccoli is known as Se accumulator. Finley , showed from an experiment, on broccoli grown in sodium selenate laden soil, that it accumulated ~ 103 μg Se/gm dry weight of the plant tissue. Broccoli is known to contain fairly  large quantity of selenium as methyl selenocysteine, perhaps due to its greater solubility in aqueous medium. However, it is strange that Se from broccoli does not accumulate efficiently in man or rats [42,43] because its major part as selenium methyl selenocysteine is perhaps metabolised to methyl selenol . It has been demonstrated experimentally that, methyl substituted forms of Se is an effective anticancer agent than the other derivatives of organo-Se compounds . Garlic with as much Se as 1000 μg/gm of dry weight has been grown  and found to contain Se as selenium methyl selenocysteine but when the Se concentration falls below 200 μg/gm, it is found as q-glutamyl selenium methyl selenocysteine. Although Se from high Se garlic is a chemoprotective agent  it is particularly selective against breast cancer in rats [48,49] induced by 7, 12-dimethyl benzene (a) anthracene. Even the aqueous solution of garlic is chemoprotective . Glucosinolate as secondary plant compound known to induce phase II enzymes  is chemoprotective against bladder cancer. Se fed experimental rats in the forms of selenite, selenate, selenomethionine did not acumulate in most rats which means it is either not absorbed or excreted through urine.
Trigonal Se nanowires and nanotubes have been synthesized in absolutely ecofriendly way. The Se nanowires of 70–100 nm width and length in several μm were prepared in absolute ethanol at room temperature while trigonal Se nanotubes of diameter 180–350 nm were obtained in aqueous medium at 85°C (358 K). It was observed that amorphous Se nanoparticles were formed in the beginning and subsequently transformed into nanowires and tubes .
Stable Se nanoparticles in colloidal form were prepared from Terminalia arjuna extract in aqueous medium. They were characterized by UV–vis, energy dispersive X-ray analysis (EDAX), transmission electron microscopy (TEM), FTIR and XRD analysis. The colloidal solution had an absorbance maximum at 390 nm. Its IR spectrum showed peaks corresponding to O-H, NH, C = O and C-O stretches suggesting the presence of hydroxyl, amino, ketonic and carbonyl functional groups in the extract which act both as stabilizer and capping agent for the Se nanoparticles . The Se nanoparticles synthesized from fenugreek seed extract in aquous medium at room temperature are between 50–150 nm. They have been found to be active against human breast cancer cells .
Se nanoparticles of approximately 35 nm have been synthesized from gum arabic which remain stable in solution for about 30 days. The gum arabic was found to be a better stabilizer for Se nanoparticles than the hydrolysed gum arabic . The Se nanoparticles synthesized from lemon leaf extract exhibited an absorption maximum at 395 nm in the UV–vis region. Initially, the mixture of leaf extract and SeO3 2− remains colourless but after stirring and incubating it for 24 hr at 30°C, it turns red . The photoluminescence spectra exhibited excitation peak at 395 and emission peak at 525 nm (Figure 3). It has been found from TEM image that the size of particles ranges between 60–80 nm. They are polydispersed in colloidal solution but crystalline in nature . The FTIR spectra of the samples with and without Se nanoparticles were compared to examine the changes in the functional groups of the biomolecules. The broad peak at 3415 due to ʋ(NH) shifts to 3418 cm−1 but new peaks appear at 2930 and 3456 cm−1 in the colloidal solution containing Se nanoparticles. The region 1500–1800 cm−1 is due to various amide bands which split into some new bands in colloidal solution. However, after reduction of the Na2SeO3 to Se nanoparticles by the biomolecules in the extract containing functional groups such as alcohol, aldehyde, phenol etc., they are oxidized to the following species:
Na2SeO3 + H2O → H2SeO3 + Na2O
H2SeO3 ⇌ SeO3 2− + 2H+
Alcohol + SeO3 2− → Se + Carboxylic acid
Aldehyde + SeO3 2− → Se + Ketone
It has also been detected from gel electrophoresis that Se nanoparticles prevented DNA damage when cells were exposed to UVB .
Polyphenol gallic acid nanoparticles from plant have been used to fabricate Se nanoparticles since the gallic acid nanoparticles may behave differently than the bulk gallic aicd. Since gallic acid can quantitatively coordinate with the Se ions, another reducing agent dithioerthreitol was added to gallic acid-coated with Se ions. A change in colour was taken as an indication of the formation of nanoparticles which was confirmed by UV–vis and emission spectroscopy . A slightly different method has also been employed by Ingole et al.  to prepare Se nanoparticles from glucose. Na2SeSO3 prepared from Se powder was treated with glucose solution according to the following chemical reactions:
Se powder + Na2SO3 → Na2SeSO3
Na2SeSO3 + H2O → H2SeO4 + Na2S
H2SeO4 ⇌ 2H++SeO4 2−
SeO4 2− + Glucose → Se + Gluconic acid
The colourless solution in the beginning becomes yellow then orange and finally turns red which does not change even after heating for over 1 h. These changes have been ascribed to the changes in size of Se nanoparticles.
Se nanoparticles from microbes, characterization and application
Microorganisms reduce the toxic, selenate and selenite oxoanions into non toxic elemental selenium which is insoluble in water. Continuous use of water or edible plants from Se rich soil can cause skin lesion and early hair fall. Effort is therefore, made to reduce Se compounds to elemental Se with the help of bacteria. It is a simple process of detoxification of selenites/selenates to Se nanoparticles as the reverse reaction is too slow to produce Se compounds .
Fast (forward reaction)
Se(IV)/Se (VI) ⇌ Se(o)
Slow (backward reaction)
Amporphous Se nanoparticles have been synthesized from sodium selenite in presence of Shewanella sp. HN-41 in aqueous medium under anaerobic conditions taking care of reaction time, selenite concentration and biomass of Shewanella. Different types of the Se nanoparticles are synthesized using protein, peptides and several other reducing agents [1,60,61]. Nanowires and nanorods have been fabricated from Rhizobium selenireducens sp., Dechlorosoma sp., Pseudomonas sp., Paracoccus sp., Enterobactor sp., Thaurea sp., Sulfurospirillium sp., Desulfovibrio sp., and Shewanella sp., [61-63]. It has been reported that the particle size is decreased in presence of O2. It is obvious that oxygen will promote oxidation of Se (forward reaction) as a consequence of which the redox step becomes slower producing smaller Se nanoparticles. Selenite reductase is also helpful in the synthesis of Se nanoparticles. A wide range of selenite concentration starting from 0.01, 0.05, 0.15, 0.25, 0.050, 0.75 and 1 mM were used to study the effect of concentration, size and morphology. Average particle size for all the above concentrations was nearly 103 ± 5.1 nm. For large quantity of nanoparticles the selenite concentration not exceeding 0.1 mM is needed. It has been observed from TEM and SAED image that Se nanoparticles are amorphous . Extracellular synthesis  of fairly smaller particles of the order of 47 nm from the fungus, Aspergillus terreus was done in 60 min.
Microbes like Klebsiella pneumoniae and Pseudomonas alcaliphila have also been used to synthesize Se nanoparticles in good yield. When Na2SeO3 was added to the activated culture of P. alcaliphila the reaction started immediately but completed after 48 h . A gradual colour change with time was observed in the following order:
Grey → Red → Intense Red
0 h 6 h 48 h
The characteristic red colour of Se nanoparticles  was detected spectrophotometrically and has been ascribed to the excitation of the surface plasmon vibration of the monoclinic Se. It has been noticed that particle size is directly proportional to reaction time and it ranges between 50–500 nm . Field emission scanning electron microscopic (FESEM) image shows nanoparticles of varying size and shape.
On the basis of above studies a possible mechanism for the formation and transformation of Se nanoballs to Se nanorods has been proposed. Zhang et al.  presume that SeO3 2− is reduced to elemental selenium by the protein excreted by the P. alcaliphila and, their aggregation gives Se nanoparticles of varying size . It is true that protein acts as reducing agent for SeO3 2− but it is available as excretion from P. alcaliphila is not convincing. The excretion contains toxins, pyrogens and traces of protein but they may not be sufficient for reduction of selenite. Authors also suggest that large m-Se nanoballs are not stable in solution and they dissolve to form Se atoms. A fraction of dissolved Se atoms crystallize as t-Se forming nanorods . It is not rue because Se in atomic state is not soluble in a solvent but stays in colloidal form. It is a misconception. However, PVP controls the size of Se nanoparticles. If the Se nanoparticles without PVP are left for 2–3 weeks they form aggregates of different shapes and size. Since SeO3 2− in ionic form is toxic to bacterial culture, the bacteria in selenite solution may therefore, die and the disintegrated protein may then act as reducing agent for selenite. The large m-Se nanoparticle can not dissolve in solution to give Se atom forming Se-nanorods. It is quite likely that they may be segregated and rearranged into nanorods.
Se nanoparticles synthesized from sodium selenite and glutathione in aqueous medium had been tested for its growth inhibition efficacy against Staphylococcus aureus. It was found that growth of S. aureus was inhibited in presence of Se nanoparticles within 3–4 h with as low concentration as 7–15 μg/ml which suggests that Se nanoparticles may be used against bacterial infections.
Biogenic Se nanoparticles fabricated from protein produced by E. coli have been compared with those synthesized from chemical reaction via redox mechanism . There are specific types of protein produced by E. coli (AdhP, Idh, OmpC, AceA) which are associated with Se nanoparticles. They are also responsible for their uniform size and distribution. One of the proteins (AdhP) was found to bind strongly to Se nanoparticles. E. coli was found to produce Se nanoparticles from 2 mM of SeO3 2− in about 48 h which was distinguished by the change in colour from colourless to dark red. The amorphous Se nanoparticles thus produced were between 10–90 nm. Since the bacterial growth continued even in presence of selenite, it is confirmed that selenite is not toxic to E. coli at this concentration. The enzymes alcohol dehydrogenase, propanol-preferring (ADHP), ACEP (Isocitrate lyase), ENO, KPYKI, IDH and GLPK require metal ions as cofactor for their activity while the enzymes DCEP, ASTC and TNAA require pyridoxal phosphate as coenzyme without which their activity is lost. The authors have not distinguished between cofactor and co-enzyme, they have termed both as metallic cofactor and non-metallic cofactor which is misleading. The cofactors in the enzyme are metal ions bonded through a coordinate covalent bond and can accept lone pair of electrons from the donor atoms in the enzyme into their vacant orbital to form the bond. The Se nanoparticle is in the elemental state and no metal in atomic state can bind to protein or any electron donating species. It is therefore; proper to use the word association of Se nanoparticles to protein rather than bonding. The size of Se nanoparticles produced in presence of the protein, alcohol dehydrogenase propanol-preferring (AdhP) were much smaller than those produced in their absence. However, since E. coli contains many other proteins than only pAdhP (purified protein), the decrease in Se nanoparticle size may be the cumulative effect of all proteins available in E. coli.
Bioreduction of selenate or selenite from microorganism such as bacteria, fungi and plant extract have become the favourite pursuit of biologist, chemist and engineers. It is expected that in future the metal would be extracted by biomineralization because they produce the purest form of the element. Many raw materials like waste vegetables, fruit peels and leather cuttings may be utilized to produce elemental metal/metalloid from their oxide, halide, nitrate, sulphide and carbonates. Generally, protein, phenol, alcohol, flavonoid or sugar are required for the reduction of SeO3 −2, SeO4 −2 and at least one of the above organic molecules is present in microbes and plant extracts. They may therefore, be exploited for the biotransformation of selenate and selenide to elemental Se of various shape and size. Since the reduced metals or metalloids are insoluble in aqueous medium they can be easily sequestered. Growth inhibition of some of the bacterial stains occurs during the redox process which suggests that selenite/selenate may be used against infection caused by such microbes.
The authors are thankful to the publishers for the permission to adopt their figures for this review.
- Dungan RS, Frankenberger T: Microbial transformations of selenium and the bioremediation of seleniferous environments. Biorem J. 1999, 3: 171-188. 10.1080/10889869991219299.View ArticleGoogle Scholar
- Tapiero H, Townsend DM, Tew KD: The antioxidant role of selenium and seleno-compounds. Biom Pharmaco. 2003, 57: 134-144. 10.1016/S0753-3322(03)00035-0.View ArticleGoogle Scholar
- Cox DN, Bastiaans K: Understanding Australian consumers’ perceptions of selenium and motivations to consume selenium enriched foods. Food Qua Pref. 2007, 18: 66-76. 10.1016/j.foodqual.2005.07.015.View ArticleGoogle Scholar
- Turakainen M, Hartikainen H, Seppanen MM: Effects of selenium treatments on potato (Solanum tuberosum L.) growth and concentrations of soluble sugars and starch. J Agric Food Chem. 2004, 52: 5378-5382. 10.1021/jf040077x.View ArticleGoogle Scholar
- Hartikainen H, Xue T, Piironen V: Selenium as an antioxidant and pro-oxidant in ryegrass. Plant Soil. 2000, 225: 193-200. 10.1023/A:1026512921026.View ArticleGoogle Scholar
- Lyons GH, Genc Y, Soole K, Stangoulis JCR, Liu F, Graham RD: Selenium increases seed production in Brassica. Plant Soil. 2009, 318: 73-80. 10.1007/s11104-008-9818-7.View ArticleGoogle Scholar
- Ip C, Thompson HJ, Zhu Z, Ganther HE: In vitro and in vivo studies of methylseleninic acid: evidence that a monomethylated selenium metabolite is critical for cancer chemoprevention. Cancer Res. 2000, 60: 2882-2886.Google Scholar
- Miller S, Walker SW, Arthur JR, Nicol F, Pickard K, Lewin MH, Howie AF, Beckett GJ: Selenite protects human endothelial cells from oxidative damage and induces thioredoxin reductase. Clin Sci. 2001, 100: 543-550. 10.1042/CS20000299.View ArticleGoogle Scholar
- Zhang Y, Zhang J, Wang HY, Chen HY: Synthesis of selenium nanoparticles in the presence of polysaccharides. Mater Lett. 2004, 58: 2590-2594. 10.1016/j.matlet.2004.03.031.View ArticleGoogle Scholar
- Xie Q, Dai Z, Huang WW, Zhang W, Ma DK, Hu XK, Qian YT: Large-scale synthesis and growth mechanism of single-crystal Se nanobelts. Crystal Growth Des. 2006, 6: 1514-1517. 10.1021/cg050493p.View ArticleGoogle Scholar
- Liu MZ, Zhang SY, Shen YH, Zhang ML: Seleniumnanoparticles prepared from reverse microemulsion process. Chin Chem Lett. 2004, 15: 1249-Google Scholar
- Quintana M, Haro-Poniatowski E, Morales J, Batina N: Synthesis of selenium nanoparticles by pulsed laser ablation. App Surf Sci. 2002, 195: 175-186.View ArticleGoogle Scholar
- Gates B, Mayers B, Cattle B, Xia Y: Synthesis and characterization of uniform nanowires of trigonal selenium. Adv Fun Mat. 2002, 12: 219-227. 10.1002/1616-3028(200203)12:3<219::AID-ADFM219>3.0.CO;2-U.View ArticleGoogle Scholar
- Wang MCP, Zhang X, Majidi E, Nedelec K, Gates BD: Electrokinetic assembly of selenium and silver nanowires into macroscopic fibers. ACS Nano. 2010, 4: 2607-2614. 10.1021/nn901923z.View ArticleGoogle Scholar
- Yang L, Shen Y, Xie A, Liang J: Oriented attachment growth of three-dimensionally packed trigonal selenium microspheres into large area wire networks. Eur J Inor Chem. 2007, 2007: 4438-4444.View ArticleGoogle Scholar
- Filippo E, Manno D, Serra A: Characterization and growth mechanism of selenium microtubes synthesized by a vapor phase deposition route. Crystal Growth Des. 2010, 10: 4890-4897. 10.1021/cg1012632.View ArticleGoogle Scholar
- Husen A, Siddiqi KS: Carbon and fullerene nanomaterials in plant system. J Nanobiotechno. 2014, 12: 16-10.1186/1477-3155-12-16.View ArticleGoogle Scholar
- Husen A, Siddiqi KS: Phytosynthesis of nanoparticles: concept, controversy and application. Nano Res Lett. 2014, 9: 229-10.1186/1556-276X-9-229.View ArticleGoogle Scholar
- Li SK, Shen YH, Xie AJ, Yu XR, Zhang XZ, Yang LB, Li CH: Rapid, room-temperature synthesis of amorphous selenium/protein composites using Capsicum annuum L. extract. Nanotechno. 2007, 18: 405101-405109. 10.1088/0957-4484/18/40/405101.View ArticleGoogle Scholar
- Gurunathan S, Kalishwaralal K, Vaidyanathan R, Venkataraman D, Pandian SRK, Muniyandi J, Hariharan N, Eom SH: Biosynthesis, purification and characterization of silver nanoparticles using Escherichia coli. Coll Surf B. 2009, 74: 328-335. 10.1016/j.colsurfb.2009.07.048.View ArticleGoogle Scholar
- Wang T, Yang L, Zhang B, Liu J: Extracellular biosynthesis and transformation of selenium nanoparticles and application in H2O2 biosensor. Coll Surf B. 2010, 80: 94-102. 10.1016/j.colsurfb.2010.05.041.View ArticleGoogle Scholar
- Oremland RS, Stolz J: Dissimilatory reduction of selenate and arsenate in nature. Environmental Metal–Microbe Interaction. Edited by: Lovley DR. 2000, ASM Press, Washington, DC, 25-Google Scholar
- Stolz JF, Oremland RS: Bacterial respiration of arsenic and selenium. FEMS Micro Rev. 1999, 23: 615-627. 10.1111/j.1574-6976.1999.tb00416.x.View ArticleGoogle Scholar
- Baesman SM, Bullen TD, Dewald J, Zhang D, Curran S, Islam FS, Beveridge TJ, Oremland RS: Formation of tellurium nanocrystals during anaerobic growth of bacteria that use Te oxyanions as respiratory electron acceptors. Appl Environ Microbiol. 2007, 73: 2135-2143. 10.1128/AEM.02558-06.View ArticleGoogle Scholar
- Oremland RS, Herbel MJ, Blum JS, Langley S, Beveridge TJ, Ajayan PM, Sutto T, Ellis AV, Curran S: Structural and spectral features of selenium nanospheres produced by Se-respiring bacteria. Appl Environ Microbiol. 2004, 70: 52-60. 10.1128/AEM.70.1.52-60.2004.View ArticleGoogle Scholar
- Narasingarao P, Haggblom MM: Identification of anaerobic selenate-respiring bacteria from aquatic sediments. Appl Environ Microbiol. 2007, 73: 3519-3527. 10.1128/AEM.02737-06.View ArticleGoogle Scholar
- Lortie L, Gould WD, Rajan S, McCready RG, Cheng KJ: Reduction of Selenate and Selenite to Elemental Selenium by a Pseudomonas stutzeri Isolate. Appl Environ Microbiol. 1992, 58: 4042-4044.Google Scholar
- Oremland RS, Blum JS, Culbertson CW, Visscher PT, Miller LG, Dowdle P, Strohmaier FE: Isolation, growth, and metabolism of an obligately anaerobic, selenate-respiring bacterium, strain SES-3. Appl Environ Microbiol. 1994, 60: 3011-3019.Google Scholar
- Sabaty M, Avazeri C, Pignol D, Vermeglio A: Characterization of the reduction of selenate and tellurite by nitrate reductases. Appl Environ Microbiol. 2001, 67: 5122-5126. 10.1128/AEM.67.11.5122-5126.2001.View ArticleGoogle Scholar
- Sharma G, Sharma AR, Bhavesh R, Park J, Ganbold B, Nam JS, Lee SS: Biomolecule-mediated synthesis of selenium nanoparticles using dried Vitis vinifera (raisin) extract. Molecules. 2014, 19: 2761-2770. 10.3390/molecules19032761.View ArticleGoogle Scholar
- Fesharaki PJ, Nazari P, Shakibaie M, Rezaie S, Banoee M, Abdollahi M, Shahverdi AR: Biosynthesis of selenium nanoparticles using Klebsiella pneumoniae and their recovery by a simple sterilization process. Braz J Microbiol. 2010, 41: 461-466. 10.1590/S1517-83822010000200028.View ArticleGoogle Scholar
- Dhanjal S, Cameotra SS: Aerobic biogenesis of selenium nanospheres by Bacillus cereus isolated from coalmine soil. Microb Cell Fact. 2010, 9: 52-10.1186/1475-2859-9-52.View ArticleGoogle Scholar
- Ingole AR, Thakare SR, Khati NT, Wankhade AV, Burghate DK: Green synthesis of selenium nanoparticles under ambient condition. Chalcogenide Lett. 2010, 7: 485-489.Google Scholar
- Klug HP, Alexander LE: X-ray Diffraction Procedures for Polycrystalline and Amorphous Materials. Wiley, New York, NY, USA, 1967.Google Scholar
- Mukherjee P, Senapati S, Mandal D, Ahmad A, Khan MI, Kumar R, Sastry M: Extracellular synthesis of gold nanoparticles by the fungus fusarium oxysporum. Chem Bio Chem. 2002, 5: 461-463. 10.1002/1439-7633(20020503)3:5<461::AID-CBIC461>3.0.CO;2-X.View ArticleGoogle Scholar
- Mukherjee P, Ahmad A, Sastry M, Kumar R: Bioreduction of AuCl4 − ions by the fungus, Verticillium sp. and surface trapping of the gold nanoparticles formed. Angew Chem Int Ed. 2001, 40: 3585-3588. 10.1002/1521-3773(20011001)40:19<3585::AID-ANIE3585>3.0.CO;2-K.View ArticleGoogle Scholar
- Lin ZH, Wang CRC: Evidence on the size-dependent absorption spectral evolution of selenium nanoparticles. Mater Chem Phys. 2005, 92: 591-594.View ArticleGoogle Scholar
- Xi GC, Xiong K, Zhao QB, Qian YT: Nucleation–dissolution–recrystallization: a new growth mechanism for t-Selenium nanotubes. Cryst Growth Des. 2006, 6: 577-582. 10.1021/cg050444c.View ArticleGoogle Scholar
- Cao XB, Xie Y, Zhang SY, Li FQ: Ultra-thin trigonal selenium nanoribbons developed from series-wound beads. Adv Mater. 2004, 16: 649-653. 10.1002/adma.200306317.View ArticleGoogle Scholar
- Finley J: Selenium from broccoli is metabolized differently than Se from selenite, selenate or selenomethionine. J Agric Food Chem. 1998, 46: 3702-3707. 10.1021/jf980027q.View ArticleGoogle Scholar
- Roberge MT, Borgerding AJ, Finley JW: Speciation of selenium compounds from high selenium broccoli is affected by the extracting solution. J Agric Food Chem. 2003, 51: 4191-4197. 10.1021/jf021247d.View ArticleGoogle Scholar
- Finley J: The retention and distribution by healthy young men of stable isotopes of selenium consumed as selenite, selenate or hydroponically-grown broccoli are dependent on the chemical form. J Nutr. 1999, 129: 865-871.Google Scholar
- Finley JW, Davis C, Feng Y: Selenium from high-selenium broccoli protects rats from colon cancer. J Nutr. 2000, 130: 2384-2389.Google Scholar
- Ganther HE, Lawrence JR: Chemical transformations of selenium in living organisms: improved forms of selenium for cancer prevention. Tetrahedron. 1997, 53: 12299-12310. 10.1016/S0040-4020(97)00561-9.View ArticleGoogle Scholar
- Ip C, Ganther HE: Activity of methylated forms of selenium in cancer prevention. Cancer Res. 1990, 50: 1206-1211.Google Scholar
- Ip C, Lisk D: Characterization of tissue selenium profiles and anticarcinogenic responses in rats fed natural sources of selenium-rich products. Carcinogen. 1994, 15: 573-576. 10.1093/carcin/15.4.573.View ArticleGoogle Scholar
- Ip C, Lisk D: Enrichment of selenium in allium vegetables for cancer prevention. Carcinogen. 1994, 9: 1881-1885. 10.1093/carcin/15.9.1881.View ArticleGoogle Scholar
- Ip C, Lisk J, Stoewsand G: Mammary cancer prevention by regular garlic and selenium enriched garlic. Nutr Cancer. 1992, 17: 279-286. 10.1080/01635589209514197.View ArticleGoogle Scholar
- Ip C, Lisk DJ: Efficacy of cancer prevention by highselenium garlic is primarily dependent on the action of selenium. Carcinogen. 1995, 16: 2649-2652. 10.1093/carcin/16.11.2649.View ArticleGoogle Scholar
- Lu J, Pei H, Ip C, Lisk DJ, Ganther H, Thompson HJ: Effect of an aqueous extract of selenium-enriched garlic on in vitro markers and in vivo efficacy of cancer prevention. Carcinogen. 1996, 17: 1903-1907. 10.1093/carcin/17.9.1903.View ArticleGoogle Scholar
- Chen H, Shin DW, Nam JG, Kwon KW, Yoo JB: Selenium nanowires and nanotubes synthesized via a facile template-free solution method. Mat Res Bull. 2010, 45: 699-704. 10.1016/j.materresbull.2010.02.016.View ArticleGoogle Scholar
- Prasad KS, Selvaraj K: Biogenic synthesis of selenium nanoparticles and their effect on As(III)-induced toxicity on human lymphocytes. Biol Trace Elem Res. 2014, 157: 275-283. 10.1007/s12011-014-9891-0.View ArticleGoogle Scholar
- Ramamurthy C, Sampath KS, Arunkumar P, Kumar MS, Sujatha V, Premkumar K, Thirunavukkarasu C: Green synthesis and characterization of selenium nanoparticles and its augmented cytotoxicity with doxorubicin on cancer cells. Bioprocess Biosyst Eng. 2013, 36: 1131-1139. 10.1007/s00449-012-0867-1.View ArticleGoogle Scholar
- Kong H, Yang J, Zhang Y, Fang Y, Nishinari K, Phillips GO: Synthesis and antioxidant properties of gum arabic-stabilized selenium nanoparticles. Int J Biol Macromol. 2014, 65: 155-162. 10.1016/j.ijbiomac.2014.01.011.View ArticleGoogle Scholar
- Prasad KS, Patel H, Patel T, Patel K, Selvaraj K: Biosynthesis of Se nanoparticles and its effect on UV-induced DNA damage. Coll Surf B. 2013, 103: 261-266. 10.1016/j.colsurfb.2012.10.029.View ArticleGoogle Scholar
- Stacey B, Sarker N, Dowdell A, Banerjee I: The spontaneous formation of selenium nanoparticles on gallic acid assemblies and their antioxidant properties. Ford Under Res J. 2011, 1: 41-46.Google Scholar
- Bajaj M, Schmidt S, Winter J: Formation of Se (0) nanoparticles by Duganella sp. and Agrobacterium sp. isolated from Se-laden soil of North-East Punjab, India. Microbial Cell Fact. 2012, 11: 64-10.1186/1475-2859-11-64.View ArticleGoogle Scholar
- Chen T, Wong YS, Zheng W, Bai Y, Huang L: Selenium nanoparticles fabricated in Undaria pinnatifida polysaccharide solutions induce mitochondria-mediated apoptosis in A375 human melanoma cells. Coll Surf B. 2008, 67: 26-31. 10.1016/j.colsurfb.2008.07.010.View ArticleGoogle Scholar
- Tam K, Ho CT, Lee JH, Lai M, Chang CH, Rheem Y, Chen W, Hur HG: Growth mechanism of amorphous selenium nanoparticles synthesized by Shewanella sp. HN-41. Biosci Biotech Bioch. 2010, 74: 696-700. 10.1271/bbb.90454.View ArticleGoogle Scholar
- Hunter WJ, Kuykendall LD, Manter DK:Rhizobium selenireducens sp. nov.: a selenite-reducing α-Proteobacteria isolated from a bioreactor. Curr Microbiol. 2007, 55: 455-460. 10.1007/s00284-007-9020-9.View ArticleGoogle Scholar
- Rathgeber C, Yurkova N, Stackebrandt E, Beatty JT, Yurkov V: Isolation of tellurite- and selenite-resistant bacteria from hydrothermal vents of the juan de fuca ridge in the pacific ocean. Appl Environ Microbiol. 2002, 68: 4613-4622. 10.1128/AEM.68.9.4613-4622.2002.View ArticleGoogle Scholar
- Morita M, Uemoto H, Watanabe A: Reduction of selenium oxyanions in wastewater using two bacterial strains. Eng Life Sci. 2007, 7: 235-240. 10.1002/elsc.200620188.View ArticleGoogle Scholar
- Klonowska A, Heulin T, Vermeglio A: Selenite and tellurite reduction by Shewanella oneidensis. Appl Environ Microbiol. 2005, 71: 5607-5609. 10.1128/AEM.71.9.5607-5609.2005.View ArticleGoogle Scholar
- Zare B, Babaie S, Setayesh N, Shahverdi AR: Isolation and characterization of a fungus for extracellular synthesis of small selenium nanoparticles. Nanomed J. 2013, 1: 13-19.Google Scholar
- Zhang W, Chen Z, Liu H, Zhang L, Gaoa P, Li D: Biosynthesis and structural characteristics of selenium nanoparticles by Pseudomonas alcaliphila. Coll Surf B. 2011, 88: 196-201. 10.1016/j.colsurfb.2011.06.031.View ArticleGoogle Scholar
- Gates B, Yin Y, Xia Y: A solution-phase approach to the synthesis of uniform nanowires of crystalline selenium with lateral dimensions in the range of 10–30 nm. J Am Chem Soc. 2000, 122: 12582-12583. 10.1021/ja002608d.View ArticleGoogle Scholar
- Tran PA, Webster TJ: Selenium nanoparticles inhibit Staphylococcus aureus growth. Int J Nanome. 2011, 6: 1553-1558.Google Scholar
- Dobias J, Suvorova EI, Bernier-Latmani R: Role of proteins in controlling selenium nanoparticle size. Nanotechno. 2011, 22: 195605-10.1088/0957-4484/22/19/195605.View ArticleGoogle Scholar
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