Fig. 3

Reversal of multidrug resistance in MDR1-overexpressing aggressive uterine cancer xenografts with complete survival of mice administrated dual sequential treatments. All minicell doses were administrated intravenously in nude mice with 10⁹ minicells per dose. The concentrations of doxorubicin or shRNA administrated per dose in 10⁹ minicells were 1011 copies of shRNA and 0.8 µg doxorubicin. Free doxorubicin was administrated at 150 µg/dose. Kaplan–Meier survival curves for the xenograft study continued for up to 120 days showing complete survival only in the MES-SA/Dx5 mice receiving sequential ^EGFRMinicell_ssMDR1 and the ^EGFRMinicells_Dox treatment or as expected in mice with the doxorubicin—sensitive MES-SA xenograft treated with ^EGFRminicells_DOX. Black, Control saline, green filled, ^EGFRMinicell_shMDR1 , blue filled, ^EGFRMinincell_Dox, pink filled, ^CMVMinicells_shMDR1  + ^EGFRMinicell_Dox, maroon filled, ^EGFRMinicell_shNonsense , purple hollow, ^EGFRMinicell_shNonsense  + ^EGFRMinicell_Dox, red filled ^EGFRMinicell_shMDR1 ^EGFRMinicell_Dox, red hollow, ^EGFRMinicell_shMDR1  + free Dox, green hollow, ^EGFRMinicell_Dox, Light blue filled, Free dox