Bacterial superglue enables easy development of efficient virus-like particle based vaccines

Table 1 Estimation of antigen coupling efficiency

Spy-VLP	Potential binding motifs	Spy-antigen	Antigen size (kDa)	Coupling efficiency (%)	Display capacity (antigens/VLP)
SpyTag	180	SpyCatcher-CIDR	32	76	136
SpyTag	180	SpyCatcher-IL-5	33	77	138
SpyTag	180	SpyCatcher-Ag85A	48	75	134
SpyTag	180	CSP-SpyCatcher	53	48	86
SpyTag	180	SpyCatcher-HER2	83	22	40
SpyTag	180	PCSK9-SpyCatcher	84	23	42
2xSpyTag	360	SpyCatcher-Survivin	30	52 (104)^a	187
2xSpyTag	360	SpyCatcher-IL-5	33	54 (107)^a	193
2xSpyTag	360	Pfs25-SpyCatcher	40	30 (61)^a	109
SpyCatcher	180	CTLA-4-SpyTag	15	88	159
SpyCatcher	180	PD-L1-SpyTag	27	45	81
SpyCatcher	180	SpyTag-VAR2CSA	118	34	61

^a% occupancy of total VLP subunits: number of displayed antigens/total number of VLP subunits (=180) × 100

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