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Table 1 Estimation of antigen coupling efficiency

From: Bacterial superglue enables easy development of efficient virus-like particle based vaccines

Spy-VLP Potential binding motifs Spy-antigen Antigen size (kDa) Coupling efficiency (%) Display capacity (antigens/VLP)
SpyTag 180 SpyCatcher-CIDR 32 76 136
SpyTag 180 SpyCatcher-IL-5 33 77 138
SpyTag 180 SpyCatcher-Ag85A 48 75 134
SpyTag 180 CSP-SpyCatcher 53 48 86
SpyTag 180 SpyCatcher-HER2 83 22 40
SpyTag 180 PCSK9-SpyCatcher 84 23 42
2xSpyTag 360 SpyCatcher-Survivin 30 52 (104)a 187
2xSpyTag 360 SpyCatcher-IL-5 33 54 (107)a 193
2xSpyTag 360 Pfs25-SpyCatcher 40 30 (61)a 109
SpyCatcher 180 CTLA-4-SpyTag 15 88 159
SpyCatcher 180 PD-L1-SpyTag 27 45 81
SpyCatcher 180 SpyTag-VAR2CSA 118 34 61
  1. a% occupancy of total VLP subunits: number of displayed antigens/total number of VLP subunits (=180) × 100