Research | Open | Published:
Bacterial superglue enables easy development of efficient virus-like particle based vaccines
Journal of Nanobiotechnologyvolume 14, Article number: 30 (2016)
Virus-like particles (VLPs) represent a significant advance in the development of subunit vaccines, combining high safety and efficacy. Their particulate nature and dense repetitive subunit organization makes them ideal scaffolds for display of vaccine antigens. Traditional approaches for VLP-based antigen display require labor-intensive trial-and-error optimization, and often fail to generate dense antigen display. Here we utilize the split-intein (SpyTag/SpyCatcher) conjugation system to generate stable isopeptide bound antigen-VLP complexes by simply mixing of the antigen and VLP components.
Genetic fusion of SpyTag or SpyCatcher to the N-terminus and/or C-terminus of the Acinetobacter phage AP205 capsid protein resulted in formation of stable, nonaggregated VLPs expressing one SpyCatcher, one SpyTag or two SpyTags per capsid protein. Mixing of spy-VLPs with eleven different vaccine antigens fused to SpyCatcher or SpyTag resulted in formation of antigen-VLP complexes with coupling efficiencies (% occupancy of total VLP binding sites) ranging from 22–88 %. In mice, spy-VLP vaccines presenting the malaria proteins Pfs25 or VAR2CSA markedly increased antibody titer, affinity, longevity and functional efficacy compared to corresponding vaccines employing monomeric proteins. The spy-VLP vaccines also effectively broke B cell self-tolerance and induced potent and durable antibody responses upon vaccination with cancer or allergy-associated self-antigens (PD-L1, CTLA-4 and IL-5).
The spy-VLP system constitutes a versatile and rapid method to develop highly immunogenic VLP-based vaccines. Our data provide proof-of-concept for the technology’s ability to present complex vaccine antigens to the immune system and elicit robust functional antibody responses as well as to efficiently break B cell self-tolerance. The spy-VLP-system may serve as a generic tool for the cost-effective development of effective VLP-vaccines against both infectious- and non-communicable diseases and could facilitate rapid and unbiased screening of vaccine candidate antigens.
Active vaccination against infectious diseases has been one of the most effective medical interventions in human history with a tremendous impact on global health. Due to safety-, manufacturing- and reproducibility concerns, global vaccine development has gradually turned its focus away from whole-pathogen based vaccines and towards recombinant subunit vaccines based on defined antigen components . The effectiveness of simple subunit vaccines is, however, considerably inferior to that of whole-pathogen-based vaccines and the successful development of soluble proteins as vaccine candidates has in many cases been a disappointment. The low immunogenicity of soluble protein antigens has been attributed to their small size (<10 nm), susceptibility to proteolytic degradation, and a low capacity for activating the innate immune system. Virus-like particles (VLPs) represent a specific class of particulate subunit vaccines, which are highly immunogenic due to sharing key characteristics with live viruses . Several VLP-vaccines have already been commercialized e.g. Engerix (hepatitis B virus) and Cervarix (human papillomavirus) by GlaxoSmithKline, Recombivax HB (hepatitis B virus) and Gardasil (human papillomavirus) by Merck, and Hecolin (hepatitis E virus) by Xiamen Innovax . VLPs are safe non-replicating shells consisting solely of viral structural proteins that, when overexpressed, self-assemble into dense multi-protein arrays with icosahedral or rod-like structures. The size of VLPs (20–200 nm) allows for direct drainage into lymph nodes and is optimal for uptake by antigen-presenting cells and cross-presentation . Their highly repetitive surface structures moreover enable complement fixation and B cell receptor clustering, altogether leading to the activation of the innate immune system, greater B cell activation and ultimately increased antibody production [4–6]. Importantly, it has been established that hetorologous antigens displayed on VLPs can assume a similar immunogenicity as the underlying particle, creating a strong rational for using VLPs as antigen-presenting platforms to increase immune responses against otherwise poorly immunogenic antigens [2, 7]. Antigen display has traditionally been achieved by either genetic fusion of heterologous epitopes into the self-assembling coat protein or by conjugation to preassembled VLPs. Genetic fusion of smaller peptides (often single epitopes) has in several cases been successful, whereas insertion of larger sequences generally prevents VLP-assembly [2, 8, 9]. Even if VLP-assembly is achieved, chimeric particles are often instable and the functional conformation of the inserted epitope may not be retained. Consequently, the genetic fusion approach is inevitably based on substantial trial-and-error optimization and is largely restricted to continuous epitopes thus requiring the pre-identification of such determinants in the target-antigen. Chemical cross-linking chemistry has been employed to conjugate target antigens to pre-assembled VLPs by stimulating covalent linkage between reactive amino acid side chains in the antigen and coat protein sequences, respectively [10, 11]. Complex antigens, however, generally present multiple reactive sites hampering consistent directional coupling of the antigen to the VLP required for optimal epitope display. In addition, such chemical reactions often result in a lower than optimal density of the VLP-displayed antigen [10, 12]. Other strategies, involving non-covalent antigen-VLP conjugation have also been pursued, each with individual advantages and disadvantages [13, 14]. The most successful general approach was developed by Cytos Biotech and involves the terminal addition of a reactive Cysteine residue to the target-antigen followed by addition of a hetero-bifunctional cross-linker to mediate coupling between the reactive sulfhydryl group (Cys) and the N-term of Lysine residues exposed on the surface of Qbeta VLPs [10, 15]. Several promising VLP-vaccines have been developed by this technology, although this method suffers from an inconsistent ability to display complex antigens with conformation-dependent epitopes. Therefore, there is a strong interest in developing new methods to obtain optimal VLP-display for complex target-antigens. Herein we report the use of the split-intein (SpyTag/SpyCatcher) conjugation system  to facilitate conjugation of target antigens to VLPs under physiological conditions. This conjugation system takes advantage of the spontaneous formation of an isopeptide bond between a Lys and an Asp present in two split units of the Streptococcus pyogenes fibronectin-binding protein FbaB. These split units consist of a peptide (SpyTag) and a protein (SpyCatcher), which in solution interact to form a highly stable amide bond. The irreversible reaction occurs within minutes and so this technology offers a simple way to conjugate antigen to VLPs. We developed a panel of genetically modified Acinetobacter phage AP205 VLPs displaying either the SpyCatcher protein (116 amino acids) or the SpyTag peptide (13 amino acids) in regular arrays. We also engineered an AP205 VLP presenting two SpyTags per VLP subunit (2xSpyTag-VLP). We characterized these spy-VLPs in terms of stability and antigen display capacity using a variety of antigens and discuss how the different spy-VLPs can be used to increase the versatility of the spy-VLP display system. Using two malaria antigens, we demonstrate that the spy-VLP system elicited high levels of high affinity IgG, which effectively inhibited key processes in the parasite development. Finally, we show that B cell self-tolerance can be overcome by the spy-VLP system, which effectively induced IgG against a range of self-antigens including PD-L1, CTLA-4 and IL-5. Thus, the data demonstrate the broad usability of the spy-VLP platform and validate its ability to facilitate strong functional antibody responses against complex vaccine antigens.
Development, expression and characterization of spy-VLPs
A panel of SpyTag or SpyCatcher presenting VLPs was designed based on the Acinetobacter phage AP205 coat protein. Expression of this protein in Escherichia coli results in the assembly of 29 nm icosahedral (T = 3) VLPs consisting of 180 subunits . Precisely, the 116 amino acid SpyCatcher sequence was fused to the N-terminus (SpyCatcher-VLP) of the AP205 coat protein (Gene ID: 956335). In addition, the 13 amino acid SpyTag peptide was fused to N-terminus (SpyTag-VLP) or to both N- and C-terminus (2xSpyTag-VLP) of the AP205 coat protein (Fig. 1a). Recombinant E. coli expression of spy-AP205 coat proteins was confirmed by SDS-PAGE analysis of fractions collected following density gradient ultracentrifugation. Reduced SDS-PAGE showed pure protein bands of expected sizes (Additional file 1: Figure S1). VLP-assembly of each spy-AP205 coat proteins was evaluated by transmission electron microscopy (TEM) (Fig. 1b) and dynamic light-scattering (DLS) analysis. For all recombinant particles the DLS analysis revealed a homogenous population of non-aggregated particles with an average estimated size of 36 nm [Pd = 12.1] (SpyTag-VLP), 42 nm [Pd = 21.6] (2xSpyTag-VLP) and 43 nm [Pd = 9.7] (SpyCatcher-VLP). In comparison, unmodified AP205 VLPs were determined by DLS to have an average size of 35 nm [Pd = 10.7].
To test if the recombinant spy-VLPs could form a covalent interaction with an antigen through their SpyTag or SpyCatcher, individual spy-VLPs were mixed with antigen fused to the corresponding binding-partner and formation of antigen-VLP subunit conjugates was subsequently confirmed by SDS-PAGE analysis. For all spy-VLP types and all tested antigens the SDS-PAGE revealed the occurrence of a protein band matching the combined size of the antigen and VLP subunit, as exemplified in Fig. 1c. Mixing of SpyTag- or SpyCatcher-fused antigen with unmodified AP205 VLPs did not produce this banding pattern (data not shown).
Versatility of the spy-VLP platform
To explore the versatility of the spy-VLP antigen display system we cloned and expressed 11 vaccine candidate antigens genetically fused to either a SpyTag or a SpyCatcher (Additional file 2: Table S1). The panel of spy-antigens, representing very diverse proteins with respect to origin, structure and size (14.5–118 kDa) included; (a) malaria proteins: CSP, CIDR, VAR2CSA, and Pfs25, which are expressed at different developmental stages of the complex life cycle of Plasmodium falciparum and used in different vaccine strategies to reduce malaria transmission or disease [18, 19]; (b) the Mycobacterium tuberculosis protein, Ag85A, in development for a tuberculosis vaccine; (c) mouse proteins involved in cancer (CTLA-4, PD-L1, Survivin and HER2), asthma/allergy (IL-5) or cardiovascular disease (PCSK9). The latter self-proteins are targets of therapies employing monoclonal antibody. The vaccine antigens were mixed with corresponding spy-VLPs and the antigen coupling efficiency (% occupancy of total VLP binding sites) and antigen display capacity (number of antigens per VLP) was estimated for each reaction by SDS-PAGE densitometric analysis. Standard molar mixing ratio was 1:1.5 (VLP:antigen) and reactions occurred over night at 4 °C. A similar coupling efficiency was observed using three-hour incubations at 37 °C (data not shown). Overall, antigen-coupling efficiencies ranged from 22–88 %, corresponding to 40–159 antigens displayed per VLP (Table 1).
There was a negative correlation between antigen size and the number of antigens bound per VLP (Spearman Rank Order Correlation Coeff. = −0.75, P = 0.02) (the 2xSpyTag-VLP constructs were excluded from the analysis). There was no significant difference in the antigen coupling efficiency in SpyTag-VLP and SpyCatcher-VLP reactions. However, the estimated coupling capacity was higher for the reaction between SpyCatcher-IL-5 and 2xSpyTag-VLP (193 antigens per VLP) compared with mixing similar amounts of SpyCatcher-IL-5 with SpyTag-VLP (138 antigens per VLP) (Table 1; Additional file 3: Figure S2D).
Immunogenicity of spy-VLP vaccines
To assess the immunogenicity of spy-VLP delivered antigens, we tested two clinically relevant malaria proteins, Pfs25 and VAR2CSA, and evaluated humoral responses in mice after intramuscular immunizations. Pfs25 is expressed on the P. falciparum ookinete surface within the mosquito. Immunization with recombinant Pfs25 formulated in Montanide ISA51 induced Pfs25-specific antibodies with capacity to block parasite infectivity to mosquitoes in a Phase 1 human clinical trial but the vaccine had unacceptable side effects . The Pfs25 antigen is poorly immunogenic by itself and development of an effective transmission-blocking Pfs25 vaccine has been hampered by the requirement of a strong adjuvant [20–22]. SpyCatcher was fused to the C-terminus of Pfs25 and Pfs25-SpyCatcher was expressed and purified from E. coli SHuffle® cells. This enabled high level expression of soluble correctly folded Pfs25-SpyCatcher as verified by binding of the transmission-blocking anti-Pfs25 monoclonal antibody, mAb 4B7  (Additional file 4: Figure S3).
VAR2CSA is a unique member of the P. falciparum erythrocyte membrane protein 1 (PfEMP1) protein family. This protein binds parasite-infected erythrocytes to placental chrondroitin sulphate A (CSA) . Anti-VAR2CSA antibodies can prevent this binding [25, 26], and clinical testing of a protein-based VAR2CSA vaccine to protect women against placental malaria has been initiated . SpyTag was genetically fused to the N-terminus of the CSA binding domain of VAR2CSA (domains DBL1-ID2a) and expressed and purified from E. coli SHuffle® cells. The protein expressed well and was folded correctly as measured by its binding to decorin, as described in .
Spy-VLP vaccine induced IgG titers
The antigen display capacities for Pfs25 and VAR2CSA were 109 and 61 proteins per VLP, respectively (Table 1; Additional file 3: Figure S2C, K). The antigen-specific IgG titer was measured by enzyme-linked immune-sorbent assay (ELISA) 2 weeks after each immunization (on days 14, 35 and 56) as well as at day 212 (Pfs25) and 137 (VAR2CSA) (Fig. 2a). The Pfs25 spy-VLP vaccine induced higher antigen-specific IgG titers than the control vaccine at all the tested time-points (P < 0.01 (day 14, 35 and 56); P = 0.03 (day 212), Mann–Whitney Rank Sum Test) (Fig. 2a). At day 212, there was a 37-fold increase in the geometric mean titer (GMT) of IgG in sera from spy-VLP vaccinated mice compared to mice vaccinated with the same amount of soluble Pfs25 plus untagged AP205 VLPs.
The GMT of VAR2CSA-specific IgG was consistently higher in the group immunized with the VAR2CSA spy-VLP vaccine compared to the control group vaccinated with uncoupled VAR2CSA. However, the difference in GMTs between the two groups was not as profound as seen in the Pfs25 study and only reached statistical significance at days 14 (P = 0.03, Mann–Whitney Rank Sum Test) and 137 (P = 0.03, Mann–Whitney Rank Sum Test) (Fig. 2a).
Avidity of spy-VLP vaccine induced IgG
In order to further examine qualitative differences in humoral responses, we investigated the avidity of IgG antibodies induced after immunizations with spy-VLP vaccines compared to control vaccines (uncoupled antigen + untagged AP205 VLPs). The avidity index values of serum IgG were determined by measuring the resistance of antibody-antigen complexes to 8 M urea by ELISA, as described . Avidity index values can be divided into three categories denoting: high avidity (avidity index values higher than 50 %), intermediate avidity (between 30 and 50 %) and low avidity (>30 %) . Prior to measuring avidity, pre-determined IgG antibody levels in individual mouse serum samples were equalized by dilution. Both the Pfs25 and VAR2CSA spy-VLP vaccines induced antigen-specific IgG with significantly higher avidity-index values compared to corresponding control vaccines P = 0.015 (Pfs25 day 35) and P = 0.032 (Pfs25 day 56) and P = 0.032 (VAR2CSA day 35) and P = 0.056 (VAR2CSA day 56), Mann–Whitney Rank Sum Test) Fig. 2b. The mean avidity index value of anti-Pfs25 sera obtained at day 35 was 46 % for the spy-VLP group, thus falling within the “intermediate avidity” category, whereas the mean value of the control group was only 22 % (i.e. low avidity). The corresponding values for day 56 were 57 % (i.e. high avidity) and 30 % (i.e. intermediate avidity) for the spy-VLP and control group, respectively. The mean avidity of antibodies induced by the VAR2CSA spy-VLP vaccine was 63 and 62 % (both high avidity) in sera obtained at day 35 and 56, respectively. In comparison, mean avidity of antibodies induced by the control vaccine was 39 and 42 % (both intermediate avidity) in sera obtained at day 35 and 56, respectively.
Subclass profiling of vaccine induced IgG
The relative proportion of IgG subclasses elicited in the spy-VLP vaccinated groups and the control groups was also compared. Measurements were performed on sera obtained at day 98 (Pfs25) or 88 (VAR2CSA) and calculations were based on ELISA measurements using subclass-specific (IgG1, IgG2a and IgG2b) secondary anti-mouse IgG antibodies for quantification. Anti-mouse total IgG secondary antibody was used for normalization. IgG1 was the dominant IgG subclass in all sera. Sera from Pfs25 vaccinated mice contained IgG1 and IgG2b and the spy-VLP vaccine induced significantly higher IgG2b (P = 0.03) and significantly lower (P = 0.03) IgG1 levels compared to the control vaccine (Fig. 2c, left). Sera from VAR2CSA vaccinated mice contained IgG of all subclasses. The distribution of IgG subclasses was similar in mice vaccinated with the two VAR2CSA vaccines. (Fig. 2c, right).
Functional activity of spy-VLP induced humoral responses
For most vaccines only a fraction of the induced IgG is biologically active in inhibiting the development of the targeted microorganism and the level and overall avidity of vaccine induced IgG responses does not necessarily reflect the anti-microbial functional activity. The goal of the Pfs-25 vaccine is to block parasite development inside the mosquito. We therefore used the standard membrane feeding assay (SMFA) to measure transmission-blocking (TB) activity of the antibodies induced by the two vaccines . The Pfs25 spy-VLP vaccine showed more than 99 % transmission-reducing activity (TRA) (one oocyst found in the 20 investigated mosquitos) compared to pre-immune serum (82 oocysts detected) or serum from mice immunized with the control vaccine (85 oocysts detected) (Fig. 3a). In a second study, BALB/c mice (n = 7) were immunized with the Pfs25 spy-VLP vaccine at days 0 and 14, and different concentrations of purified IgG from pooled anti-Pfs25 serum samples were subsequently tested in the SMFA assay. At the highest IgG concentration (750 µg/ml) serum from the Pfs25 spy-VLP vaccinated mice completely blocked oocyst formation (Table 2).
Serum concentrations of 250 and 83.3 µg/ml showed more than 95 % transmission-reducing activity compared to IgG purified from ovalbumin immunized mice (Table 2).
The aim of VAR2CSA vaccines is to induce IgG, which inhibit the binding between parasite-infected erythrocytes and placental chondroitin sulfate. We therefore compared the vaccines ability to elicit IgG inhibiting the binding between VAR2CSA expressed on infected erythrocytes and chondroitin sulfate in an in vitro assay (Fig. 3b). The mean EC50 calculated from the dose–response curve of serum from VAR2CSA spy-VLP immunized mice was eightfold higher, [CI 95 % 3.213–14.41], than the mean EC50 in serum from mice vaccinated with VAR2CSA that was not bound to VLPs (Fig. 3b).
Breaking self-tolerance by spy-VLP display
To examine the capacity of the spy-VLP system to overcome B cell self-tolerance and induce autoantibody responses upon vaccination with self-antigens, mouse proteins PD-L1, CTLA-4 and interleukin-5 were recombinantly expressed with SpyTag or SpyCatcher and formulated as spy-VLP vaccines. PD-L1 and CTLA-4 down regulate T cell function. Expression of these proteins in tumors has been linked to poor prognosis and antibody-targeting of the proteins is showing promise in cancer treatment . Antibody measurements of anti-sera obtained at day 56 showed that the PD-L1 and CTLA-4 spy-VLP vaccines induced significant antibody responses against the self-antigens compared to the soluble protein (P = 0.01 and P < 0.01, respectively) (Fig. 4a, b).
The therapeutic use in severe asthma of antibodies targeting IL-5 is supported by abundant data from in vitro experiments, animal models and clinical trials . The SpyCatcher was fused at the N-terminus of mouse IL-5. Antibody measurements of anti-IL-5 in sera obtained at day 56 showed that the spy-VLP vaccine induced significant antibody responses against this self-antigen compared to the control vaccine (P = 0.02) (Fig. 4c). The autoantibody response elicited by the spy-VLP vaccine did not decay rapidly as the titers measured at day 112 (Fig. 4d) were similar to those measured at day 56 (P = 0.02) (Fig. 4c).
Mixing SpyCatcher-IL-5 with 2xSpyTag-VLPs resulted in a higher antigen coupling capacity than when mixing the same antigen with SpyTag-VLPs (Table 1; Additional file 3: Figure S2D). These two mixtures were administered to mice to compare IgG levels elicited by the two vaccines. The GMT titer of sera obtained at day 35 from mice vaccinated with the IL-5 2xSpyTag-VLP vaccine (GMT = 580) was 13 fold higher than for the anti-IL-5 SpyTag-VLP sera (GMT = 41) (P = 0.06, Mann–Whitney Rank Sum test), suggesting that the increased antigen coupling capacity of the 2xSpyTag VLP had an effect on the immunogenicity of the vaccine (Additional file 5: Figure S4).
Virus-like particles (VLPs) are effective at establishing both prophylactic and therapeutic immunity against their source virus or foreign antigens displayed on their surface. Aiming at developing a versatile VLP-based antigen display platform, we designed a panel of genetically modified spy-AP205 coat proteins, which assembled into stable, non-aggregated VLPs presenting reactive SpyTag or SpyCatcher on their surfaces. The Acinetobacter phage AP205 was chosen as platform as AP205 VLPs have been shown to tolerate fusion of heterologous peptides at both the N- and C-terminus of the structural protein  and because AP205 VLPs can be produced at low cost in E. coli. The spy-VLPs were assessed in terms of their applicability to bind and display antigens using the SpyCatcher/SpyTag conjugation system . This protein-coupling tool offers several key advantages. SpyTag and SpyCatcher polypeptides react and form an irreversible isopeptide bond at high yield under a variety of conditions (including variation in pH, temperature or buffer composition) , which offer ample possibilities for antigen-specific optimization. We have previously experienced that antigen-VLP conjugates tend to form aggregates and precipitate upon mixing of antigen and VLP. Subsequent modification of buffer conditions can prevent this while still allowing efficient conjugation (data not shown). Notably, a similar split-intein conjugation system (Snooptag/SnoopCatcher) was recently reported, which may be used in a similar strategy for conjugation of antigen to pre-assembled VLPs .
Having developed both SpyTag and SpyCatcher fused VLPs; either binding partner can be chosen as fusion-tag to the antigen. In most cases the SpyTag peptide will be the preferred choice since the minor addition of 13 amino acid residues to the antigen is unlikely to negatively influence protein expression or polypeptide folding, which in turn could have impact on the functional capacity of elicited IgG. Interestingly, for several proteins, we observed increased expression yields after fusing the SpyCatcher to the antigen, creating an additional rational for employing SpyTag-VLPs for conjugation of SpyCatcher-fused antigens. Specifically, we were able to express soluble and correctly folded Pfs25-SpyCatcher using E. coli SHuffle® (NEB Biolabs) strains. Notably, E. coli expression of soluble Pfs25, in functional conformation, has not previously been reported  and expression of naked Pfs25 using similar E. coli SHuffle® cells resulted, in our hands, in formation of inclusion bodies (data not shown).
Epitope density is an important factor for B cell activation . It was thus interesting to examine whether the spy-VLP system presented vaccine antigens at high density. The VLPs formed by structural proteins to which one SpyTag or SpyCatcher were fused provided 180 binding sites per VLP. We tested 11 spy-antigens in different combinations with these spy-VLP platforms. The antigen density per particle varied according to antigen and the VLP employed, but the mean display capacity was 118 antigen molecules per spy-VLP, corresponding to that antigens had been coupled to 65 % of the available VLP subunits. We had expected antigen-specific characteristics (e.g. net surface charge) would have a greater effect on the coupling efficiency than what was observed, but the observed correlation between antigen size and the density of antigen display on the VLPs, suggests that steric hindrance plays a role. The VLPs with 180 available antigen binding sites elicited robust IgG responses, however we also designed VLPs where each subunit protein had two SpyTags attached. These VLPs had 360 binding sites available. The immunogenicity of IL-5 was higher when administrered with the high density VLP (193 antigens coupled per VLP) than with the lower density VLP (138 antigens coupled per VLP), but the difference only achieved borderline statistical significance (P = 0.06). Due to close proximity of the SpyTags on the VLPs with 360 binding sites it is likely that smaller proteins are better able to bind effectively to these particles, whereas larger proteins face problems of steric hindrance. Thus each of the three developed spy-VLP platforms contributes to the versatility of the antigen display system.
The long-term success of a Pfs25-based transmission-blocking vaccine depends upon induction of high and sustainable levels of functional antibody that effectively block parasite development in the mosquito [36, 37]. In this regard, a single immunization with bivalent HPV16/18 L1 VLP vaccine (Cervarix) has been shown to induce protective antibody levels remaining stable for more than 48 months . This has been attributed to the ordered, repetitive, and dense display of epitopes on the VLP surface. We found, that the immunogenicity of Pfs25 on spy-VLPs was high and that the anti-Pfs25 IgG levels was considerably higher 6 months post immunization compared to mice vaccinated with uncoupled protein, although IgG levels showed a tendency to decline throughout the study period. Further long-term studies are thus needed to establish if anti-Pfs25 levels will eventually plateau and, in that event, assay the transmission-blocking activity of the remaining antigen-specific IgG.
Anti-VAR2CSA IgG titers were not boosted to the same extent by the spy-VLP display as seen in the Pfs25 study. Likely, this is explained by the fact that soluble VAR2CSA is a superior immunogen i.e. the soluble protein induced relatively high antigen specific IgG titers. However, the comparably lower antigen display capacity of the VAR2CSA spy-VLP vaccine (61 antigens per VLP) may also have limited immunogenicity. Importantly, a vaccine to protect against placental malaria should be administered prior to conception i.e. to pre-puberty girls. Therefore, several years are likely to lapse between vaccination and exposure. It is thus important to note that anti-VAR2CSA IgG titers were significantly higher in the spy-VLP immunized group compared to the control when examining serum samples obtained at day 137.
VLP display had a significant positive effect on the avidity of the humoral response against both Pfs25 and VAR2CSA. The avidity index values determined for anti-Pfs25 IgG in sera from mice immunized with soluble Pfs25 formulated in aluminum hydroxide were categorized as “low” to “intermediate” affinity whereas IgG in corresponding serum samples from Pfs25 spy-VLP immunized mice were categorized as “high affinity”. The avidity of anti-VAR2CSA IgG was also higher after vaccination with VLPs compared to vaccination with uncoupled protein. This might be of importance since the avidity of anti-VAR2CSA IgG has been associated with protection against placental malaria . This might seem somewhat surprising as one might predict that VLP display could lower the average avidity of induced antibodies since the multivalent interaction of the displayed antigen with B cell receptors would lead to activation of lower affinity B cells. However, the ability of virus-like display to break B cell peripheral self-tolerance and activate anergic B cells, perhaps via signaling through IgD, may contribute to the observed higher avidity. These activities would permit the generation of antibody secreting plasmablasts through tolerized naïve B cells and self-reactive intermediates generated during somatic hypermutation and this would not be the case with monomeric antigen. This situation could result in a more diverse antibody repertoire that would drive selection of higher affinity clones [40–42].
Comparison of IgG subclasses revealed a significantly (2–2.5 fold) higher induction of anti-Pfs25 IgG2b in Pfs25 spy-VLP vaccinated mice compared to the control group (such a difference was not observed in the VAR2CSA study). In mice, IgG1 is associated with a T-helper cell type 2 (Th2)-like response, while a Th1 response is associated with the induction of IgG2a, IgG2b, and IgG3 antibodies . IgG2b (and IgG2a) exhibit the strongest binding to Fc receptors  and has a higher capacity for complement fixation than IgG1 . Both Pfs25 vaccines were formulated in aluminum hydroxide (Statens Serum Institut, Copenhagen, Denmark), which is a known stimulator of Th2 responses. It is interesting that the Pfs25 spy-VLP vaccine was able to induce a significantly higher IgG2b level, indicating a boosted Th1 response. The IgG subclasses can contribute to clearance of pathogens by different mechanisms. Accordingly, the induction of a broad subclass and a balanced Th1/Th2 response might be desirable for some vaccines. AP205 VLPs expressed in E. coli contain host cell RNA, which can activate the innate immune system via TLR 7 and 8. Besides, endotoxin levels were not measured in the VLP preparations. It is thus a possibility that bacterial RNA and/or endotoxins have affected the profile of IgG subclasses induced by the spy-VLP and unconjugated control vaccine, respectively.
For Pfs25, the spy-VLP vaccine was far more potent than the vaccine based on soluble protein in the ability to elicit biologically functional antibodies. Indeed when our results are benchmarked against other Pfs25 vaccination studies the tested Pfs25 spy-VLP vaccine shows state-of-the-art efficacy [34, 46, 47]. Similar findings were observed for VAR2CSA, the functional parasite binding-inhibition assay showed an eight fold higher parasite binding-inhibition capacity in sera from spy-VLP vaccinated mice compared to control sera. In conclusion, the results of the Pfs25 and VAR2CSA immunization studies demonstrate proof-of-concept for the spy-VLP system to increase the functional antibody responses to complex vaccine antigens.
Display of self-antigens on VLPs is an effective approach for inducing strong antibody responses against self-antigens providing opportunities for development of therapeutic VLP-based vaccines [48–50]. We thus examined if the spy-VLP system was efficient in breaking self-tolerance. These studies, based on both cancer (PD-L1 and CTLA-4) and allergy (IL-5) associated self-antigen targets, demonstrated a consistent ability of the spy-VLP mediated antigen-display to facilitate induction of potent autoantibody responses. Previously, VLP-display induced autoantibody responses have been reported to wane over time with a typical half-life in humans and non-human primates of ~3 months [51–53] and there has been no evidence for boosting by endogenous self-antigen, probably since foreign T cell helper responses are required to stimulate antibody production. It is thus interesting that our IL-5 spy-VLP vaccine induced antigen-specific autoantibody titers, which did not show a significant decline over a period of 4 months post immunization.
Reporting work, done in parallel and unknown to us, Brune et al. recently published on the development of a SpyCatcher-AP205 VLP, which was used in a similar strategy to ours to develop VLP-vaccines based on two malaria antigens (Pfs25 and CIDR) . In that study, proof-of-principle was established alone by showing increased antigen-specific IgG titers in sera obtained approximately 2 weeks after immunization of mice with the spy-VLP vaccines compared to a control vaccine. Our study extends these results by presenting different spy-VLP platforms, which are each used to characterize the systems versatility for antigen display. Furthermore, spy-VLP-induced responses in mice were analyzed over several months and included measurement of antibody titer, avidity and subclass distribution. Finally, we demonstrate proof-of-concept for the spy-VLP system to increase functional antibody responses against different pathogen antigens as wells as to overcome B cell self-tolerance.
The requirement of simple subunit-based vaccines to be administered with an extrinsic adjuvant, of which only a few have been approved for human use , is an impediment for vaccine development. VLP-display of vaccine antigens might mitigate this requirement and enable development of adjuvant-free vaccines. A generic VLP-display platform may moreover be instrumental for screening of vaccine candidate antigens since especially low-immunogenic or cryptic antigens (e.g. Pfs25, HPV L2, PfRH5) depend greatly on increased immunogenicity for their protective potential. The ability of the spy-VLP system to display whole antigens enables induction of polyclonal antibody responses, which may have a superior neutralizing capacity compared to the essentially monoclonal responses induced by epitope-based vaccines. Finally, the simple production of VLP vaccines that have been made possible by the versatile spy-VLP system raises the possibility that the spy-VLPs could be pre-manufactured and distributed as a generic tool for use in laboratories working on vaccine development against a wide variety of target antigens.
Design, expression and purification of spy-AP205 virus-like particles
The Acinetobacter phage AP205 coat protein (Gene ID: 956335) was used to design three “spy-VLPs”, presenting either SpyTag or SpyCatcher on their surface. SpyTag-VLP was constructed by adding the SpyTag peptide sequence (AHIVMVDAYKPTK) to N-terminus of the AP205 coat protein. 2xSpyTag-VLP had the SpyTag added to both the N- and C-terminus of the AP205 coat protein. SpyCatcher-VLP had the SpyCatcher protein added to the N-terminus of AP205 coat protein. The constructs were all designed to contain a flexible linker between the binding tag and the AP205 coat protein; GSGTAGGGSGS (SpyTag-VLP), GGSGS (SpyCatcher-VLP), GSGTAGGGSGS (N-terminus of 2xSpyTag-VLP) and GTASGGSGGSG (C-terminus of 2xSpyTag-VLP). Gene sequences were further modified to contain NcoI and NotI restriction sites at the N- and C-termini, respectively, and were codon-optimized for recombinant expression in E. coli before being synthesized by (GeneArt® Life Technologies, Germany). All spy-VLP expression sequences were cloned into a pET-15b vector and transformed into One Shot® BL21 Star™ (DE3) (Thermo Scientific) cells. Transformed colonies were screened by small-scale (10 mL) protein expression followed by SDS-PAGE analysis to identify the transformed clones with the highest level of protein expression. Expression was done in 3L shake flasks containing 400 mL 2xYT media (100 µg/mL ampicillin). Bacterial cultures were incubated for approximately 3 h (OD600 = 0.6) at 37 °C before they were induced with 1 mM IPTG and then allowed to incubated for additional 16 h at 20 °C. Cultures were harvested by centrifugation (10,000g) and pellets were resuspended in 1xPBS (pH = 7.2) and lysed by sonication at 80 % power with 5 pulsations for 2 × 5 min on ice. Cleared bacterial lysates were then purified by ultracentrifugation (UC) through an Optiprep™ (Sigma) step (23, 29 and 35 %) gradient, modified from . In brief, 1.2 ml bacterial lysate was loaded on top of the gradient in Polyallomer Centrifuge open top tubes (11 × 60 mm) (Bechmann Coulter) and spun at 307.900 RCF (SW60Ti rotor, Beckmann Coulter) for 3.30 h at 16 °C. UC fractions were subsequently analyses by SDS-PAGE and VLP-containing fractions were pooled and dialyzed in PBS (0.02 % PS80, pH 7.2) using a 300.000 MWCO membrane (Spectrum). Dialyzed VLP samples were finally spun down at 16,000g for 2 min at 4 °C to remove aggregates. Protein concentration was determined by bicinchoninic acid assay (Sigma), following manufacturer’s instructions.
Using the droplet method, an aliquot of VLPs was diluted to 0.2 mg/mL in PBS. Diluted VLPs were adsorbed to carbon and negatively stained with 2 % phosphotungstic acid (pH = 7.0) for 1 min. A grid was placed on the carbon floating on top of the 2 % phosphotungstic acid stain droplet. Excess stain was removed with filter paper. The sample was examined with a CM 100 BioTWIN electron microscope (Phillips) at an accelerating voltage of 80 kV. Photographic records were obtained on an Olympus Veleta camera.
Particle size measurement by dynamic light scattering (DLS)
VLPs were diluted to 0.2 mg/mL in PBS and spun at 15,000g for 10 min at 4 °C to remove aggregates. 70 µl of each VLP sample was loaded into disposable low volume cuvettes and mounted into the DLS chamber. Size distribution was obtained by DLS measurements at 25 °C using WYATT Technology, DynaPro NanoStar, equipped with a 658 nm laser. Each sample was measured twice with 20 runs. The mean size of the most predominant particles in the population was calculated together with the % polydispersity (% Pd).
Design, expression and purification of spy-antigen
All vaccine constructs were designed with a VLP binding-tag (SpyCatcher or SpyTag) and a hexa histidine purification tag at opposite termini of the antigen. A flexible serine/glycine linker was inserted between the spy binding-tag and the antigen. Gene sequences codon-optimized for expression in E. coli were modified to contain NcoI and NotI restriction sites at the N- and C-terminus, respectively. Gene sequences codon-optimized for expression in Spodoptera frugiperda insect cells were designed with BamHI and NotI restriction sites at the N- and C-terminus, respectively. All sequences were synthesized by (GeneArt® Life Technologies, Germany). A complete list of antigens used in this study is shown in (Additional file 2: Table S1). For expression in E. coli One Shot® BL21 Star™ (DE3) (Thermo Scientific) or SHuffle® T7 Express (C3029H New England BioLabs) cells, gene sequences were cloned in into a pET-15b vector and expressed, as described previously (see Expression and purification of spy-AP205 virus-like particles). For expression in baculovirus-transfected insect cells gene fragments were cloned into the BamHI/NotI sites of the pAcGP67A vector (BD Biosciences). To generate recombinant virus particles, linearized BakPak viral DNA (BD Biosciences) was co-transfected with pAcGP67A/Avi-L1 into Sf9 insect cells using Lipofectamine 2000 10 Reagent (Invitrogen, 11668–019) and incubated at 28 °C for 3–5 days. Recombinant baculovirus was harvested from the supernatant and used to generate a high-titer virus stock, which was used for infection of High-Five insect cells. Infected High-Five cells were incubated for 48 h at 28 °C with shaking. Expression in S2 insect cells were done as previously described .
Conjugation of vaccine antigens to VLPs
To attach vaccine antigen to VLPs, spy-VLPs and spy-antigen were incubated overnight at 4 °C at a 1:1.5 M ratio. Antigen and VLPs were mixed in a standard phosphate-buffered saline buffer supplemented with 0.2 % Polysorbate 80, pH 7.2. To prevent aggregation/precipitation of the antigen-VLP conjugate it was sometimes necessary to optimize buffer conditions by adjusting the pH and/or ionic strength of the buffer (PD-L1 VLP vaccine was mixed in PBS, 0.8 M NaCl, pH 6.2). Coupling efficiencies reported in Table 1 were estimated by densitometric analysis of SDS-PAGE gels (Additional file 3: Figure S2) using ImageQuant TL software. The percentage of VLP subunits, which had been conjugated to an antigen via interaction between SpyTag and SpyCatcher (% coupling efficiency), was estimated by dividing the intensity value of the VLP subunit protein band before reaction with antigen with the corresponding intensity value after reaction with antigen, multiplied with 100. This percentage was subsequently multiplied by 180 or 360 to calculate the antigen-display capacity (number of antigens/VLP).
Immunization of mice
Mice were immunized with the described VLP vaccines or control vaccines prepared in a similar way as the VLP vaccines, but where the Spy-tagged vaccine antigens were incubated with untagged AP205 VLPs. Thus, the only difference between the VLP vaccines and the control vaccines was that vaccine antigens were coupled to the VLPs in the VLP vaccines, whereas the control vaccines contained soluble vaccine antigen and uncoupled VLPs. Stock solutions of spy-VLPs contained 1 mg/ml spy-AP205 protein and were estimated by SDS-PAGE to be >95 % pure. All vaccines, except the VAR2CSA based vaccines, were formulated with 2 % Alhydrogel (Statens Serum Institut, Copenhagen, Denmark) to a final concentration of 2 mg/ml aluminum hydroxide. The Alhydrogel was added 1 h prior to intramuscular immunization (50 µL/mice). The VAR2CSA vaccines were diluted with PBS and administrated without adjuvant. The indicated vaccine dose refers solely to the amount of antigen. Vaccines were administered on days 0, 21 and 42. Blood samples were obtained 2 weeks after the first, second and third immunizations. Additional blood samples were collected according to the vaccine antigen as follows: Pfs25 vaccines on days 98 and 212; VAR2CSA vaccines on days 88 and 137; IL-5 vaccines on day 112. BALB/c mice were used for the VAR2CSA and IL-5 immunization studies as well as for generating anti-Pfs25 sera used in the SMFA study testing different IgG concentrations (Table 2). C57/BL6 mice were used in the CTLA-4 and PD-L1 study as well as in the other Pfs25 immunization study (Figs. 2, 3).
Antibody response measured by standard ELISA
Serum IgG levels were measured by standard enzyme-linked immunosorbent assay (ELISA). 96-well plates (Nunc MaxiSorp) were coated over night at 4 °C with vaccine protein, without SpyTag or SpyCatcher component, (1 µg/ml in PBS). Plates were blocked with 1 % BSA buffer for 1 h at room temperature (RT). Mouse serum diluted 1:100 in blocking buffer were added in three-fold dilutions to triplicate wells and incubated for 1 h at RT. Plates were washed three times in PBS with 0.05 % TWEEN 20 in between different steps. Horseradish peroxidase (HRP)-conjugated polyclonal goat anti-mouse IgG (A16072, Life Technologies, Denmark) was diluted 1:3000 in blocking buffer and incubated for 1 h. Finally, color reactions were developed for 7 min by adding o-phenylenediamine substrate. The HRP enzymatic reaction was terminated by addition of 2.5 M H2SO4 and the optical density was measured at 490 nm using an ELISA plate reader (VersaMax Molecular Devices). Serum IgG endpoint titers were estimated using a cutoff of OD490 = 0.2.
Serum IgG subclass profiling by ELISA
IgG subclass profiling of anti-Pfs25 and anti-VAR2CSA mouse sera followed a standard ELISA protocol, as described above. However, serum IgG levels were first normalized based on pre-determined ELISA OD490 values by dilution. Subsequent to incubation of IgG-normalized serum samples, secondary HRP-conjugated antibodies were used for detection of total mouse IgG (A16072, Life Technologies, Denmark) along with mouse IgG subclasses; IgG1 (A10551, Thermo Fisher), IgG2a (M32307, Thermo Fisher) and IgG2b (M32507, Thermo Fischer). For each serum sample, the OD490 value obtained by detection with subclass-specific anti-mouse IgG secondary antibodies was divided by the OD490 obtained by detection with anti-mouse total IgG secondary antibody. These relative measures were finally used for comparison of IgG subclass profiles in anti-Pfs25 and anti-VAR2CSA sera from spy-VLP immunized and mice vaccinated by the uncoupled soluble protein control vaccines.
Measuring avidity of serum IgG
Recombinant Pfs25 or VAR2CSA (1 µg/ml in PBS) was coated on Nunc MaxiSorp plates overnight at 4 °C. Plates were incubated with blocking buffer (1 % BSA) for 1 h at room temperature (RT). Plates were washed three times in between different steps. Before adding the anti-sera for incubation with the capture antigen, IgG levels in individual serum samples were equalized by dilution. This was done based on pre-determined OD490 ELISA values. IgG-normalized serum samples were added (50 µl per well) to the ELISA plate in triplicates, and incubated for 1 h at RT. The ELISA wells were subsequently washed three times in PBS with 0.05 % TWEEN 20 and 50 µl of freshly made 8 M Urea was then added to the wells for 5 min (reference plates were incubated with PBS). ELISA plates were then washed three times with PBS (0.05 % TWEEN 20). HRP-conjugated polyclonal goat anti-mouse IgG (A16072, Life Technologies, Denmark) was diluted 1:3000 in blocking buffer and incubated for 1 h. Finally, color reactions were developed for 7 min by adding o-phenylenediamine substrate. The HRP enzymatic reaction was stopped by adding 2.5 M H2SO4 and the optical density was measured at 490 nm using an ELISA plate reader (VersaMax Molecular Devices). IgG avidity index values were calculated as the ratio of the mean OD value of urea-treated wells to PBS control wells multiplied by 100.
Purification of IgG from anti-Pfs25 mouse serum
Total IgG was purified using Protein G columns (Pierce, USA) from anti-Pfs25 mouse serum samples. Briefly, protein G columns were equilibrated with binding buffer (Immunopure IgG, Pierce, USA) after which a 1:1 mixture of sera and binding buffer was allowed to flow through under gravity, the columns were then washed and eluted with elution buffer (Immunopure IgG, Pierce, USA). The eluted fraction was collected in 1 M Tris–HCl (pH 9.0, Teknova, USA) and transferred for buffer exchange to Amicon centrifugal filters (Millipore, USA) using PBS. The eluent was concentrated in PBS (Invitrogen, UK) and filtered using a 0.22 μm Millipore Ultrafree sterile centrifugal unit.
Standard Membrane-Feeding Assay (SMFA)
Two independent SMFA analyses were performed. The first SMFA analysis was done to compare the transmission blocking (TB) activity of anti-Pfs25 sera obtained by immunization of mice with the Pfs25 spy-VLP vaccine or with the corresponding control vaccine. This SMFA experiment was conducted as previously described [30, 58]. Briefly, 30 μl of mouse serum obtained at day 56 was mixed with 90 μl of naïve human serum and 150 μl of in vitro gametocyte cultures of the P. falciparum (NF54) laboratory line. The mixture was fed to Anopheles stephensi mosquitoes through a membrane feeding apparatus. Pre-immune sera served as controls. Fully engorged mosquitoes were separated and held at 26 °C. Seven days later, midguts of 20 mosquitoes were examined for oocysts. The observed transmission reducing activity of serum was determined as the percentage reduction in the median oocyst number in test samples compared to that in controls. The experiment was considered valid when at least 85 % of the mosquitoes feeding on control sera were infected. The other SMFA study, testing different concentrations of IgG purified from anti-Pfs25 spy-VLP mouse sera, was done as described . Briefly, mature P. falciparum strain NF54 Stage V gametocytes (adjusted to parasiteamia of 0.15 % ± 0.05 %) were mixed with different concentrations of purified IgG from Pfs25 spy-VLP vaccinated mice. This mixture was then fed to 4–6 day old starved female A. stephensi (SDA 500) mosquitoes via a parafilm® membrane. The mosquitoes were maintained at 26 °C and 80 % relative humidity. After 7 days mosquitoes (n = 20 per group) were dissected and the number of oocysts counted per mosquito midgut was recorded. Percent reduction in infection intensity was calculated relative to the respective control IgG (anti-ovalbumin mouse serum) tested in the same assay.
Inhibition of binding assays
Plasmodium falciparum (FCR3 genotype) parasites were maintained in culture as described . Parasites were panned on BeWo cells to select for a chondroitin sulphate A (CSA)-binding phenotype, as described . Parasite DNA was labeled with Tritium by overnight incorporation of titrated hypoxanthine. A 96-well plate (Falcon) was coated with 2 μg/ml of Decorin (Sigma-Aldrich) overnight and blocked with 2 % bovine serum albumin (Sigma) as described . Tritium labeled late-stage infected erythrocytes (IEs) were MACS purified and added to the 96-well plate in a concentration of 200,000 cells per well. Titrations of serum were added in a total volume of 100 μl in triplicate wells. After incubation for 90 min at 37 °C, unbound IEs were washed away by a pipetting robot (Beckman-Coulter). The remaining IEs were harvested onto a filter plate (Perkin-Elmer). After addition of scintillation fluid (Perkin-Elmer) the counts per minute (CPM) recording the number of non-inhibited IE was determined by liquid scintillation counting on a Topcount NXT (Perkin-Elmer). Data were adjusted to percentage of binding by dividing test result with the mean value of wells with IE incubated without serum.
All statistical analysis was done using non-parametric, two tailed, Mann–Whitney Rank Sum Test. Statistical significance was defined as P < 0.05.
The animal studies were approved by the Danish Animal Experiments Inspectorate. Approval number: 2013-15-2934-00902/BES.
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ST and CMJ performed all experiments, except WR, MVDVB, GJVG and RS performed the SMFA, SM, WADJ, SC and MR generated vaccine antigens and MAN performed the parasite-binding-inhibition assay. ST, TG, JTS, TGT, AS and AFS designed the experiments. AFS and ST wrote the paper. All authors analyzed the data. All authors read and approved the final manuscript.
The authors would like to thank Jens Hedelund Madsen, Anne Corfitz, Elham Marjan Mohammad Alijazaeri and Nahla Chehabi for technical assistance. We would like to thank Susheel Kumar Sinhg for the kind donation of mAb 4B7 and The Biophysics Facility—Protein Structure and Function Program from Center for Protein Research, Copenhagen, for assisting us with DLS measurements. We would like to thank Dr. Carole Long and Dr. Kazutoyo Miura, NIH, for assisting with SMFA analysis, and the PATH Malaria Vaccine Initiative for their funding support to the NIH for this effort. This study was supported by DANIDA, IdMalVac from the Danish Research Councils (Grant 13127), PlacMalVac under the European Union Seventh Framework Programme, FP7-HEALTH-2012-INNOVATION under grant agreement no. 304815 and through a grant from Bill and Melinda Gates Foundation Grant ID APP178218.
ST, CMJ, TG, MAN, TGT, AS and AFS are inventors on a patent application covering the described technology.