Fig. 6

Improved ATP content in human lung fibroblasts exposed to CDPs and antioxidants. Human lung fibroblasts (MRC-5 cell line) were exposed to a fine particles or b ultrafine particles at a concentration of 20 µg/cm² for 24 h at 37 °C. Next, a three different antioxidants (NAC, Vit. C and Vit. E for 2 h, 2 times 3 h and 24 h, respectively) or b Vit. C were added using the concentrations indicated in the graphs. Intracellular ATP content was determined by Cell-Titer glow luminescent cell viability assay. Positive control (PC), corresponding fine or ultrafine particles were added and negative control (NC) plain, complete culture medium was added. Data are represented as means ± SD (n = 5). * Statistically different from control p < 0.05