Fig. 5

A Quantification of GAG secretion of BMSCs by DMMB assay. B–D qRT-PCR was performed to detect the chondrogenic-related genes expression of Col2a1, ACAN, SOX9, Col1a1and Col10a1. E The secretion of collagen type II (Col2a1) was detected by immunofluorescence. Values are presented as the means ± SD, n = 3, different letters denote significances with P < 0.05