Table 2 Performance of colorimetric-based DNAzyme-assisted aptasensors for cancer detection
From: The recent advancements in the early detection of cancer biomarkers by DNAzyme-assisted aptasensors
Biomarkers/cells | Strategy | Sample | Aptamer Sequence (5′ → 3′) | NM/NPs | LOD | LR | Refs. |
|---|---|---|---|---|---|---|---|
PDGF | PGM-based DNAzyme | Saliva | CAGGCTACGGCACGTAGAGCATCACCATGATCCTGTTTT | Fe3O4 NPs | 0.11 fM | 3.16 × 10− 16 M to 3.16 × 10− 12 M | [86] |
CEA | Split DNAzyme/Hemin/ABTS | Saliva | GGGTAGGGCGGGTTGGG | - | 1 ng mL− 1 | 1–50 ng mL− 1 | [87] |
MUC1 | Magnetic NPs/DNAzyme/hemin/H2O2/ABTS | Serum | TGGGTAGGGCGGGTTGGGAAA | Magnetic NPs | 5.08 nM in a PBS 5.60 nM in serum | 50-1000 nM | [93] |
VEGF | DNAzyme/hemin/H2O2/ABTS | Serum | CAGACAAGAGTGCAGGGTTT TTTTTTT | - | 1.70 pM | 24 pM to 11.25 nM | [94] |
K-ras gene | DFA-machine/DNAzyme/hemin/H2O2/ABTS | Cell suspension | Sense primer: CCTGCTGAAAATGACTGAA Anti-sense primer: CATATTCGTCCACAAAATG | - | 10 pM | 0.01 to 150 nM | [96] |
BRCA1 gene | Pb2+-DNAzyme/RCA/HP | Buffer | Target DNA: GAACAAAAGGAAGAAAATC Hairpin probe: AGAAAATCATCTCTTCTCC GAGCCGGTCGAAATAGTGGGTGATTTTCTTCCTTTTGTTC | Magnetic beads (MBs) | 3.3 fM | - | [97] |
PTK7-positive CCRF-CEM cell/ | aptamer sgc8c/HRP-DNAzyme/ABTS | suspension | ssDNA-1: ATCTAACTGCTGCGCCGCCGGG AAAATACTGTACGGTTAGACCCAACCC ssDNA-2: TGGGTAGGGCGGGTTGGGTC TAACCGTACAGTA-3’ | - | 500 cells | - | [99] |