Skip to main content

Table 1 Experimental data on binding rate between KVLFF-functionalized MNPs and Aβ1− 42 molecules when they are mixed in 1:1 numerical ratio in the experimental device. Data refer to amyloid concentration, as measured by Lumipulse®, in the supernatant solution after the magnets completed two rotations to cover the two-loop spiral as detailed in the text. The binding ratio is calculated as the difference between the initial concentration and the one after the process

From: Magnetic separation and concentration of Aβ 1–42 molecules dispersed at the threshold concentration for Alzheimer’s disease diagnosis in clinically-relevant volumes of sample

Aβ concentration

(µg /ml)

MNP

concentration

(µg/ml)

Numerical ratio

(Aβ/MNP)

Dilution

factor

Initial Aβ concentration

- \(\varvec{A}\varvec{\beta }0\) -

(pg/ml)

*after dilution

Final Aβ concentration

-\(\varvec{A}\varvec{\beta }\varvec{f}\) -

(pg/ml)

Binding rate (%)

\(1-\frac{\varvec{A}\varvec{\beta }\varvec{f}}{\varvec{A}\varvec{\beta }0}\)

REAL CONCENTRATION

RAW EXPERIMENTAL RESULTS

 

0.001

5

1:1

1/5

408

8

98

0.001

5

1:1

1/5

422

60

86

0.001

5

1:1

1/5

398

22

94

0.001

5

1:1

1/5

403

62

85

  1. Binding ratio average: 90%