Table 4 Experimental data on binding rate between KVLFF-functionalized MNPs and Aβ1−42 molecules when they are mixed with different numerical ratios in the experimental device. The experimental procedure behind is the same as for the data in Table 1
Aβ concentration (pg/ml) | MNP concentration (µg/ml) | ratio (Aβ/MNP) | Dilution factor | Initial Aβ concentration - \(\varvec{A}\varvec{\beta }0\) - (pg/ml) *after dilution | Final Aβ concentration -\(\varvec{A}\varvec{\beta }\varvec{f}\) - (pg/ml) | Binding rate (%) \(1-\frac{\varvec{A}\varvec{\beta }\varvec{f}}{\varvec{A}\varvec{\beta }0}\) | Average |
|---|---|---|---|---|---|---|---|
REAL CONCENTRATION | RAW EXPERIMENTAL RESULTS | ||||||
600 | 3 | 1:1 | 1/3 | 220 | 51 | 77 | 78% |
600 | 3 | 1:1 | 1/3 | 231 | 47 | 80 | |
600 | 3 | 1:1 | 1/3 | 248 | 57 | 78 | |
600 | 3 (bare MNP) | 1:1 | 1/3 | 202 | 172 | 15 | 15% |
200 | 3 | 1:3 | 1/3 | 82 | 7 | 91 | 88% |
200 | 3 | 1:3 | 1/3 | 61 | 7 | 89 | |
200 | 3 | 1:3 | 1/3 | 80 | 12 | 85 | |
200 | 3 (bare MNP) | 1:3 | 1/3 | 67 | 54 | 20 | 20% |
1800 | 3 | 3:1 | 1/3 | 544 | 384 | 30 | 28% |
1800 | 3 | 3:1 | 1/3 | 546 | 409 | 25 | |
1800 | 3 | 3:1 | 1/3 | 529 | 363 | 31 | |
1800 | 3 (bare MNP) | 3:1 | 1/3 | 476 | 407 | 15 | 15% |
60 | 3 | 1:10 | 1/3 | 19 | 5 | 75 | 79% |
60 | 3 | 1:10 | 1/3 | 17 | 3 | 83 | |
60 | 3 | 1:10 | 1/3 | 20 | 4 | 80 | |
60 | 3 (bare MNP) | 1:10 | 1/3 | 16 | 14 | 13 | 13% |