- Open Access
The impact of nanoparticle-driven lysosomal alkalinization on cellular functionality
Journal of Nanobiotechnology volume 16, Article number: 85 (2018)
The biomedical use of nanosized materials is rapidly gaining interest, which drives the quest to elucidate the behavior of nanoparticles (NPs) in a biological environment. Apart from causing direct cell death, NPs can affect cellular wellbeing through a wide range of more subtle processes that are often overlooked. Here, we aimed to study the effect of two biomedically interesting NP types on cellular wellbeing.
In the present work, gold and SiO2 NPs of similar size and surface charge are used and their interactions with cultured cells is studied. Initial screening shows that at subcytotoxic conditions gold NPs induces cytoskeletal aberrations while SiO2 NPs do not. However, these transformations are only transient. In-depth investigation reveals that Au NPs reduce lysosomal activity by alkalinization of the lysosomal lumen. This leads to an accumulation of autophagosomes, resulting in a reduced cellular degradative capacity and less efficient clearance of damaged mitochondria. The autophagosome accumulation induces Rac and Cdc42 activity, and at a later stage activates RhoA. These transient cellular changes also affect cell functionality, where Au NP-labelled cells display significantly impeded cell migration and invasion.
These data highlight the importance of in-depth understanding of bio-nano interactions to elucidate how one biological parameter (impact on cellular degradation) can induce a cascade of different effects that may have significant implications on the further use of labeled cells.
The biological behavior of nanoparticles (NPs) is currently receiving much attention, in particular to enhance our understanding of any potential hazards involved in NP exposure and to optimize the use of nanotechnology in biomedical applications [1,2,3]. Most studies to date involve the use of cell cultures as a good model system that can provide in-depth mechanistic insight into the precise nature of how the cells interact with the engineered NPs . Other advantages of using cell culture models are the need for less animal studies which greatly enhances the speed with which the assays can be performed, while also reducing the number of animals required for in vivo studies. Novel technologies are being implemented to further increase the capacity to perform nanotoxicological research at high speeds, including automated high-content imaging, transcriptomics and proteomics [5,6,7,8].
The big efforts made have generated large amounts of data, which can be used to decipher the precise mechanisms by which NPs interact with their biological environment [9,10,11,12,13]. The wide variety in different types of NPs and conditions used for exposure of the NPs to their biological environment results in the generation of highly specific data that is relevant to a particular NP formulation used under very specific conditions. Although these specific mechanisms are very interesting and need to be investigated, more emphasis has recently been put on large-scale comparative studies of highly similar NP formulations . These studies either enable researchers to link particular biological effects to one single NP-associated parameter , or define new general paradigms by which NPs can affect biological systems .
Based on the data obtained, several paradigms have been defined which appear to be vital in how the cell reacts to the presence of any NPs. The generation of oxidative stress has been shown to be involved in most types of NPs among a wide array of cell types . As different cell types have different levels of natural antioxidants such as glutathione to defend themselves against the damages incurred from elevated levels of reactive oxygen species (ROS) , any elevation in ROS does not immediately result in cell death, depending on the extent of ROS produced and the nature of the cell type used . A second paradigm lies in the possible biodegradation of the NPs when subjected to the degradative microenvironment of the cellular endosomal network . Several types of NPs (e.g. ZnO, CuO, Ag) have shown to display pH-dependent dissolution properties and when internalized by the cells through endocytosis, the acidic endosomal lumen can promote NP degradation [19, 20]. The degradation is then linked to the release of potentially toxic metal ions, which can cause cell death [6, 19, 20]. It remains somewhat a matter of debate to what extent any observed effects are either due to the NPs themselves, the metal ions already present in the extracellular medium due to pre-dissolution of the NPs at neutral pH, or the metal ions released intracellularly after cellular NP uptake . In most cases, all three components will contribute to the observed cellular effects, but intracellularly released ions have been suggested to locally reach high levels which can exceed toxic thresholds and hereby induce cellular damage at levels where free metal ions that distribute more homogenously do not cause such effects .
A third paradigm is the disturbance of cellular autophagy levels through NP exposure . The precise nature of this effect remains rather unclear and is the topic of interest in a wide number of studies [21,22,23]. Initially, several groups suggested that a large number of NPs were capable of inducing autophagy and result in so-called autophagic cell death [24, 25]. Although cell death through autophagy-related mechanisms is possible, the autophagy community has labelled autophagic cell death a misnomer , as it was often based on the wrong interpretation of results. Autophagy is primarily a self-protective mechanism, where any cell that is undergoing stress resulting in damaged organelles (e.g. mitochondria) can result in the induction of autophagy to clear the damaged organelles and recycle their constituents for other cellular processes . As such, any dead cell can show signs of elevated autophagy as a secondary effect as the cell was simply trying to defend itself and recover from the damage to which it finally succumbed. More recent studies have however shown that high levels of autophagy can result in cell death as the cells is literally “eating itself” . From the NP community, similar confusing findings have been reported, where in most studies it has been shown that NPs cause cell death through autophagy, while several other studies have reported the induction of autophagy as a cellular defense mechanism to cope with the NPs [28, 29]. Any such effects may be in part explained by differences in cellular NP levels, where initially, autophagy induction may be self-protective and inhibit apoptotic signaling, while at higher levels, apoptosis itself can result in cell death . Another point of confusion lies in whether autophagy is actively induced  or whether it is related to an accumulation of autophagosomes due to a reduced clearance by lysosomes . Although autophagy disturbances have been associated to a wide variety of NPs , it is not yet considered a general mechanism either.
Autophagy is a cellular degradation mechanism and may come into play when the overall degradative capacity of the cell is reduced . In the present work, we employ gold and silicon dioxide NPs of similar size and surface charge as models agents to study the contribution of the composition of NP cores on their cellular effects, with a special emphasis on any disturbances in the capacity of cellular degradation and associated signaling.
Results and discussion
In the present study, commercially available gold and silicon dioxide NPs were used. The NPs had actual sizes of 25.3 and 20.4 nm, for gold and SiO2 respectively, as measured by transmission electron microscopy (TEM) (Fig. 1). In phosphate buffered saline, their hydrodynamic diameters were 39.8 and 35.7 nm, indicating high colloidal stability, which is likely bestowed by their negative surface charges of − 27.1 and − 35.4 mV, respectively. No endotoxins were found in the NP suspensions as determined by a LAL-assay. The small hydrodynamic diameter also suggests the high monodispersity of both samples and the lack of any substantial aggregation, which was further supported by the low polydispersity index (0.131 and 0.168, respectively). In the present work, the NPs were used to label two commonly used cell types, being human bronchial epithelial cells (BEAS-2B) and murine mesenchymal stem cells (MSCs).
Nanoparticle-mediated cell death and oxidative stress
As a first step, both cell types were exposed to a wide concentration range of either NP, after which a wide number of cellular parameters were evaluated using a validated high-content imaging based procedure [5, 6, 14]. Figure 2 reveals concentration-dependent loss of cell viability with is linked with an increase of cell membrane damage. Under our conditions, significant toxicity was only observed at concentrations of 200 µg/ml for both the Au and SiO2 NPs, in either of the cell types used. As no significant membrane damage was observed at subcytotoxic conditions, this suggests that the observed damage was a secondary effect of the dying process, rather than being a NP-induced mechanism, as has been observed for other NPs, such as hydrophobically-capped Au NPs [14, 32, 33]. At the highest subcytotoxic concentration, both NPs induced significant levels of oxidative stress, which is in line with literature data [34, 35]. Oxidative stress can cause a variety of secondary effects, but as mentioned above, is not always correlated with cell death, due to the cellular antioxidant capacities which can differ widely between different cell types . Here, for both NP types, oxidative stress correlated nicely with the onset of mitochondrial stress, indicating cellular damage, which may eventually have led to the observed cell death at higher NP concentrations. Mitochondria are the main energy providers for the cell, but their metabolic processes such as oxidative phosphorylation also generate ROS, making them susceptible to additional forms of oxidative stress . Stressed and damaged mitochondria can directly result in cell death, as mitochondrial leakage and release of cytochrome c is known to be a key component in the intrinsic apoptotic pathway . Together, these data suggest that the oxidative stress and associated mitochondrial damage incurred by cellular NP internalization are likely the underlying causes of the observed cell death at higher NP concentrations.
Nanoparticle-mediated effects on cellular autophagy and endosomal network
Apart from inducing apoptosis, damaged mitochondria may also induce autophagy in an attempt to self-preserve . Figure 2 shows clear concentration-dependent increases in cellular autophagy levels. Interestingly, although the levels of oxidative stress and mitochondrial damage were similar for the Au and SiO2 NPs, the level of autophagy induction was more outspoken for Au NPs (Fig. 3a, b). When cells were treated with N-acetyl cystein (NAC), a ROS scavenger, the level of oxidative stress and mitochondrial damage were completely reduced to control levels for both Au and SiO2 NP-treated cells (Fig. 3a, b) like what has been observed for TiO2 based nanoparticles . However, the level of autophagy was only completely reduced for SiO2 NP-treated cells, whereas Au NP-treated cells displayed a clear but not complete reduction of cellular autophagy levels. This finding suggests that the elevated autophagy levels for Au NP-treated cells were not solely caused by induction due to mitochondrial damage, but were also influenced by other factors. Other studies on gold NPs have revealed that they may induce an alkalizing effect on the endosomal system, which may reduce the functionality of the endosomes and hereby limit clearance of autophagosomes, resulting in an accumulation of autophagy-related vesicles . To verify this, the size of the endosomal network and the average pH of the endosomes were monitored, showing no significant changes in either of these factors for cells exposed to SiO2 NPs. Au NP-treated cells however showed an enlarged endosomal network and an increase in endosomal pH (Fig. 2). These data suggest that the activity of the endosomes in which the Au NPs are localized may be affected. This was further confirmed by performing a lysosomal activity assay (Fig. 3c), which revealed a clear drop in lysosomal activity for Au NP-treated cells. From a mechanistic point of view, Au NPs could cause a loss of cellular degradative capacity by means of steric hindrance, where the persistent presence of non-biodegradable NPs within the endosomal network may diminish the overall degradative capacity of the cell. Alternatively, Au NPs have also been found to affect endosomal pH by interfering with the composition of the vacuolar H+(V)-ATPase, which regulates lysosome acidification. This ATPase is composed of a membrane-associated ion conductance Vo protein complex and a peripherally associated ATPase V1 protein complex, and Au NPs have been found to result in a dissociation of both components, hereby reducing its functionality . The observed differences here between Au and SiO2 NPs suggests that the nature of the inorganic core plays a pivotal role in this alkalinization effect. This effect is likely to occur less for NPs that are prone to degradation (e.g. Ag, FexOy, ZnO, CuO) and even SiO2, which has also been found to degrade in an aqueous environment, albeit more rapidly under more alkaline conditions . The impact of the coating of the NPs on these processes remains unclear, where the impact on differences in cellular uptake levels, intracellular distribution and persistence against biodegradation may all affect cellular degradation capacities.
Deactivation of the lysosomes will have an impact on the cellular degradative capacity, which can result in changes in cellular signaling, enhancing cellular autophagy levels in a manner to overcome the loss in degradative capacity. Interestingly, the activity of the lysosomes in SiO2 NP-treated cells was found to slightly increase (Fig. 3c). Lysosomal activation has been rarely reported for NP-treated cells, but has indeed already been observed for silica NP-exposed cells . Lysosomal activation likely stems from the intrinsic nature of these organelles and the manner by which they deal with foreign pathogens. Changes in endosomal network have been described to stem from cellular conditioning to the presence of the NPs in an attempt to properly handle the influx of these foreign compounds .
Nanoparticle-mediated effects on cellular cytoskeleton network
Autophagy has also been shown to affect other cellular processes, such as for instance, the tubulin network . Analysis of cell size and skewness through staining of cytoskeletal networks revealed a clear loss of cytoskeletal organization, which resulted in shrinkage and shape-changes of cells exposed to the Au NPs at subcytotoxic conditions (Fig. 2). Interestingly however was that these alterations were not observed for SiO2 NP-treated cells (Fig. 2). Despite a difference in the degree of autophagy, SiO2 NPs still induced significant levels of autophagy, but this has no effect whatsoever on the cytoskeletal network. Previous studies have reported clear effects of various NPs including iron oxide, silver and gold on cell size and cytoskeletal network, predominantly affecting actin fibers [6, 44,45,46,47], while this effect was not observed for silica NPs . Silica NPs have however been observed to affect tubulin fibers . As silica NPs were also found to activate lysosomes , this may suggest an involvement of the lysosomal activity status on the precise nature of the cytoskeletal changes observed.
Both actin and tubulin network are involved in a wide array of cellular processes ranging from structural support to mediators in various intracellular signaling pathways . The effect of any NPs on these networks may therefore have profound effects on various signaling pathways, which could turn into a loss of cell migration, cell differentiation or even result in cell death [45, 49]. One of the key mediators in cytoskeletal signaling are the focal adhesion complexes (FAC) which connect the actin cytoskeleton to transmembrane integrin receptors and transmit external signals through various protein complexes . Here, we observed a significant loss of FAC for Au NP-treated cells, while no difference was observed for SiO2 NP-treated cells (Fig. 2). The functional implications of the loss of FACs were portrayed by the decrease in cell division rate for Au NP-treated cells, whereas no alterations in cell division times were observed for SiO2 NP-treated cells (Fig. 3d).
Cellular alterations caused by lysosomal deactivation
Alterations to the cell size and shape have been frequently reported to accompany cellular NP uptake and have been associated to changes invoked by alterations required for NP endocytosis or through steric hindrance caused by the intracellular presence of large volumes of NPs sequestered in the endosomal compartment [44, 52]. To verify whether this is a transient phenomenon, the effect of the NPs on cell size was analyzed at several time points post cellular NP exposure. Figure 4a reveals that SiO2 NP did not have any effect at any time point, while the Au NPs resulted in significant but reversible effects. These effects were however delayed, where maximal effects occurred 2 days after NP exposure, while cells were nearly recovered after 5 days post NP exposure. The delayed effect suggests that the cytoskeletal changes were not inherent to the endocytic processes through which the cells took up the NPs, but rather hinted at transient cellular alterations. As SiO2 NPs did not cause any such effects, the elevated autophagy levels and loss of lysosomal activity seen for Au NP-treated cells may be involved. A near identical pattern was observed upon analysis of cellular FACs (Fig. 4b), where SiO2 NPs had only minimal effects, whereas Au NPs resulted in significant but transient reduction of total cellular FAC sizes. These data suggest that any functional effect observed will also be transient in nature. The latter may explain the high level of discrepancy observed for various NPs, mainly iron oxide NPs and their effect on stem cell differentiation. Various research groups have observed clear inhibition of chondrogenesis for iron oxide NP-labelled MSCs, while other groups saw no effect of the iron oxide NPs, even when identical commercial particles were used [53,54,55,56]. As the differentiation protocol takes rather long (up to 2 weeks), but can vary between different protocols used, the occurrence of any inhibition may be explained by the time window used and whether cells had sufficiently recovered from their transient functional impediments.
The mitochondrial stress incurred by the Au or SiO2 NPs may cause some metabolic disorders and reduce the overall cellular energy levels as mitochondria are the main sources of cellular ATP . The induction of autophagosomes which can engulf the damaged organelles and then fuse to lysosomes to promote the degradation of their contents produces new metabolites that can be used as sources of energy . Promoting the cellular degradation capacity, as observed for the PS NP-treated cells can therefore be seen as a cellular attempt to restore any loss in cellular energy levels. To test this hypothesis, cellular ATP levels were measured for SiO2 and Au NP-treated cells (Fig. 4c), which revealed a clear transient loss of cellular ATP for Au NP-treated cells, while no effect was noticed for SiO2 NP-treated cells. For Au NPs, the process of energy restoration appears to be flawed. The loss of degradative capacity impedes the fusion of the autophagosomes and lysosomes and thus cannot restore any ATP. The alkalizing effect of the Au NPs on the endosomes can further decrease cellular ATP levels as maintenance of lysosomal pH is an ATP-dependent process .
Mechanisms underlying cellular changes through lysosomal deactivation
The loss of FACs, cytoskeletal deformations and decrease in cell division rates in Au NP-treated cells may be linked to the observed changes in cellular degradative capacity (autophagy induction) and cellular ATP levels. We tested the activity levels of both Cdc42, Rac and RhoA, which are important GTPases involved in cytoskeletal organization and cell cycle progression . The data reveal that SiO2 NP-treated cells, as expected, did not show any significant changes in the activity status of either of the three GTPases (Fig. 5).
Au NP-treated cells displayed clear activation of Cdc42 and Rac immediately after cell labeling while RhoA activity decreased. This is in line with the known functions of Cdc42 and Rac, which increase FAC turnover and hereby reduce the number of FAC. After 2 days, the activity levels of Cdc42 and Rac decrease to return to normal baseline levels after 4 days, while the activity of RhoA increases and is significantly elevated 4 days after cellular NP exposure, and then also returns to baseline levels at 6 days post NP exposure. These findings are perfectly in line with our observed kinetic alterations of the cellular cytoskeleton network. Based on known activators of the different GTPases, the following mechanism can be hypothesized (Scheme 1). The three GTPases are known to be carefully regulated to provide tight control over FAC turnover. Cdc42 and Rac activation increases FAC turnover while RhoA activation increases actin stress fiber formation and decreases FAC turnover . Cdc42 and Rac have been described to be activated through autophagy induction , while their activation typically reduces RhoA activity . As the cellular degradative capacity is reduced and autophagosomes cannot be efficiently cleared through lysosomal fusion, autophagy induction is likely to only be short-term, resulting in the decrease of Cdc42 and Rac activity levels with time. While Cdc42 and Rac activities go down, RhoA activity is able to rise and get back to normal levels. However, the low levels of cellular ATP triggers RhoA activity  and makes it significantly elevated above baseline levels. The restoration of cellular mitochondria and increase in ATP levels will finally stabilize RhoA activity back to baseline levels. The initial elevated Cdc42 and Rac activity will cause the cytoskeletal deformations and impede cell division, while the elevated RhoA activity at a later stage will restore FACs, actin stress fibers and cell cycle progression rates. Most effects observed here for the Au NP-treated cells are therefore transient ones that are related to the loss of lysosomal degradation capacity. Division of the cells and the associated dilution of the NPs amongst the two daughter cells helps the cells to recover and return to normal baseline physiological levels.
Overall, the Au NP-elicited effects are mainly caused by the precise chemical nature of the NP and the combination of several factors, being the induction of oxidative stress resulting in mitochondrial damage, the induction of autophagy and the loss in lysosomal activity inhibiting autophagosome clearance. These parameters must all occur under specific conditions which do not inflict any cell death and will therefore only happen with certain NPs under specific conditions that are capable of inducing the right level of oxidative stress, autophagy and impede lysosomal activity. The affected cellular signaling pathways can however have great implications for cell functionality, including loss of stem cell differentiation capabilities, cell division rates or cell migration properties which warrants their careful evaluation [44, 49].
Functional effects caused by differences in cell signaling pathways
The affected activity of the GTPases can also result in losses of cellular functioning, as these GTPases are key signaling mediators in a broad number of cellular signaling pathways . Here, the effect of the NPs on cell migration was studied, which is an important functional property for both cell types. For alveolar epithelial cells like Beas-2B, cell migration plays a pivotal role in airway repair and remodeling involved in respiratory diseases such as asthma , while MSC are, amongst others, the cellular source of fracture healing and are recruited to bone fracture sites . Figure 6 shows that the SiO2 NPs did not have any significant effect on the migration or invasion efficacy of either the Beas-2B or MSC cells, while at subcytotoxic concentrations, the Au NPs significantly impeded both migration and invasion. These findings are completely in line with the differences in cytoskeletal alterations and affected cell signaling pathways. The reduced migration and invasion may be explained by the reduced ATP levels caused by the Au NPs, as low levels of cytoplasmic ATP reduce the function of vacuolar H+-ATPases, which has been shown to be associated with a reduction in cellular migration and invasion .
Together, these data indicate how relatively minor differences in the initial biological effects of both NP types can result in large differences in cellular functionality. This knowledge can be very useful for a wide range of biomedical applications. Firstly, if time permits, cells can be labelled with Au NPs and then used for functional studies when sufficient time has passed and cellular functionality has been restored. If the migratory capacity of the cells is important, Au NPs appear to be less suitable than SiO2 NPs. While both NPs are frequently being used as drug delivery vehicles, the nature of the drug may determine which type of NP would be better suited. For acid-labile agents that rapidly degrade under acidic conditions, the Au NPs may offer some protection through the alkalinization of the lysosomal lumen. Pro-apoptotic drugs may be better combined with SiO2 NPs, as the higher levels of autophagy for Au NPs may impede the pro-apoptotic signaling induced by the drugs. Autophagy-inducing anticancer drugs may then be more optimally suited for use with the Au NPs, where the pro-autophagy effects of both the carrier and the drug can be combined.
The present work highlights the importance of a full comprehension of bio-nano interactions to explain any observed functional behavior in biological components. Here, gold and SiO2 NPs had rather similar toxicity profiles, where they differed in one seemingly minor parameter, being the lysosomal activity. However, coupled with the other cellular alterations, including mitochondrial ROS and autophagy induction, this parameter became very important. For SiO2, lysosomal activity slightly increased, enabling an efficient clearance of autophagosomes and recycling of autophagy-processed cellular materials, such as mitochondria. This led to an efficient clearance of damaged mitochondria and replacement by new ones. For Au NPs, lysosomal activity was decreased, which impeded clearance of the autophagosomes. This led to the activation of Rac and Cdc42, which affect cytoskeletal organization and actin-mediated signaling, slowing down cell division. This also resulted in a less efficient turnover of the damaged mitochondria, resulting in a loss of ATP, which activated RhoA signaling. Initially, Rac and Cdc42 activity inhibited RhoA activity, but upon recovery of the cellular degradative capacity, and decrease of Rac and Cdc42 activity, RhoA activity became more dominant. This resulted in a recovery of the baseline cytoskeletal architecture and actin-mediated signaling levels. Simultaneously, the increased cellular degradative capacity increases the turnover of damaged mitochondria and restores cellular ATP levels, which finally reduces RhoA activity back to baseline levels. These data reveal that a minor difference in biological impact of NPs can, in combination with other toxicological effects, result in a wide range of altered signaling pathways which can have a broad range of functional implications. In the present study, the clear impact on cytoskeletal alterations was easily noticed, but more subtle differences can often be overlooked. Here, the effects were only transient, indicating the need for more kinetic studies to further elucidate the impact of NPs on biological components.
Nanoparticles and characterization
Gold and silica NPs were obtained commercially via NanoComposix, Ltd (product numbers AULB20-5M and SISN20-25M, respectively). The gold NPs were provided with lipoic acid, while silica NPs had free silanol groups at their surface. The purchased NPs were both 20 nm diameter according to the company.
In-house characterization was performed, including analysis of size [transmission electron microscopy (TEM)], hydrodynamic size and surface charge (dynamic light scattering) and sterility tests (LAL-endotoxin assay). For the TEM specimen preparation, a drop of nanoparticle solution was dropped on the Cu-grid with C-film for the TEM investigation. All the grids with the sample were dried at RT followed by scanning the large regions of the grid. The low and high resolution TEM of the sample were examined using transmission electron microscopy (TEM) on a FEI Titan 80/300 microscope equipped with a Cs corrector for the objective lens, a Fischione high angle annular dark field detector (HAADF), GATAN post-column imaging filter and a cold field emission gun operated at 300 kV as an acceleration voltage. Electrophoretic mobilities and hydrodynamic radii were measured with a Zetasizer ZS90 instrument (Malvern, UK) at 25 °C. To optimise the response for every given sample, the samples were diluted with PBS (1/100) immediately prior to the measurement. The LAL-assay was performed according to the manufacturer’s protocol (Pierce).
Human bronchial epithelial cells (BEAS-2B) were grown in high glucose containing Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 10% fetal calf serum, 1 mM sodium pyruvate, 2 mM l-Glutamine and 1% penicillin/streptomycin (Gibco, Invitrogen, Belgium). The BEAS-2B cells were passaged upon reaching 80% confluence and reseeded at a ratio of 1:5.
Mouse mesenchymal stem cells (MSCs) were maintained in high glucose containing Dulbecco’s modified Eagle’s medium (DMEM), supplemented with 10% fetal calf serum, 10% horse serum, 1 mM sodium pyruvate and 2 mM l-Glutamine (Gibco, Invitrogen, Belgium). Cells were passaged when reaching nearly 80% confluence and reseeded at a density of 100,000 cells/flask in 75 cm2 tissue culture flasks (Nunc, Belgium).
High-content analysis of cell-nanoparticle interaction studies
For high-content imaging studies, both cell types were seeded at 7500 cells/well in a 24 well plate (Nunc, Belgium). Cells were allowed to attach overnight in a humidified atmosphere at 37 °C and 5% CO2, after which the cells were incubated with either the Au or PS NPs for 24 h in full growth medium. For cellular exposure studies, cells were incubated with the NPs at 10, 25, 50, 75, 100, 125, 150, 175 and 200 µg/ml. Each experiment was performed in three independent repeats and data were analyzed using full data sets of the different repeats. The different assays were performed as described elsewhere [5, 14].
The generation of heat maps was performed using conditional formatting of Excel sheets after z-normalization of all the data. Data are expressed as the fold increase of a certain parameter (cell death, membrane damage, mitochondrial ROS, mitochondrial stress, cell area, cell skewness, autophagy levels, endosomal size, endosomal pH, focal adhesion complexes) compared to the control levels where the level of increase is indicated by colour-codes.
Evaluation of RhoA, Cdc42 and Rac1 activity
MSC and Beas-2B cells were seeded in 25 cm2 collagen-coated tissue culture flasks at 1 * 105 cells/flask and allowed to settle overnight. Next, cells were incubated with fresh media (10 ml) containing the Au or SiO2 NPs at 150 µg/ml for 24 h. Media were removed, cells washed twice with ice-cold PBS, and cells were then either kept in culture for an additional 2, 4 or 6 days or processed immediately. At these time points, cell lysates and GTPase activities were prepared and measured according to the manufacturer’s instructions (RhoA, Rac1, Cdc42 G-LISA activation kits, Cytoskeleton Inc, Denver, USA). Absorbance was recorded at 450 nm with an ELISA plate reader (Optima FluoStar, BMG LabTech GmbH, Ortenberg, Germany). For all three cell types, the results obtained for the NP-treated samples were normalized against the value obtained for untreated control cells at identical protein levels. Values are expressed as relative to those obtained for untreated controls cells (= 1) for a total number of three independent repeats.
Measurement of cellular ATP levels
MSC and Beas-2B cells were seeded in 25 cm2 collagen-coated tissue culture flasks at 1 * 105 cells/flask and allowed to settle overnight. Next, cells were incubated with fresh media (10 ml) containing the Au or SiO2 NPs at 150 µg/ml for 24 h. Media were removed, cells washed twice with ice-cold PBS, and cells were then either kept in culture for an additional 4 days or processed immediately. To lyse the cells, 1 ml of ice-cold lysis buffer was added per flask (100 mM Tris + 4 mM EDTA, pH 7.5) after which the lysates were processed according to the manufacturer’s instructions (ATP Determination kit, Thermo Fisher Scientific, Waltham, MA, USA). Luminescence was measured using the IVIS Spectrum well plate option 10 min after addition of d-luciferin. For all three cell types, the results obtained for the NP-treated samples were normalized against the value obtained for untreated control cells at identical protein levels. Values are expressed as relative to those obtained for untreated controls cells (= 1) for a total number of three independent repeats.
Lysosomal activity measurements
MSC and Beas-2B cells were seeded in 25 cm2 collagen-coated tissue culture flasks at 1 * 105 cells/flask and allowed to settle overnight. Next, cells were incubated with fresh media (10 ml) containing the Au or SiO2 NPs at 150 µg/ml for 24 h. Media were removed, cells washed twice with ice-cold PBS, after which cell lysates were prepared and acid phosphatase activity measured according to the manufacturer’s instructions (Acid Phosphatase assay kit, Sigma-Aldrich, St. Louis—MO, USA). For all three cell types, the results obtained for the NP-treated samples were normalized against the value obtained for untreated control cells at identical protein levels. Values are expressed as relative to those obtained for untreated controls cells (= 1) for a total number of three independent repeats.
Cell migration and invasion
Both cell types were seeded in 25 cm2 collagen-coated tissue culture flasks at 1 * 105 cells/flask and allowed to settle overnight. Next, cells were incubated with fresh media (10 ml) containing the Au or SiO2 NPs at 150 µg/ml for 24 h. Media were removed, cells washed twice with ice-cold PBS, after which they were reseeded in new 24 well plates at a density of 1 * 104 cells/well, either the Radius™ 24 well cell migration assay plate (Cell Biolabs Inc, San Diego, CA, USA) or a 8 µm-pore Boyden chamber (Cell Biolabs Inc, San Diego, CA, USA). After 24 h, the gel plug was removed from the Radius migration assay plate, allowing the cells to migrate. To promote migration of the Beas-2B cells, they were exposed to 20 ng/ml IL-6. For cell invasion studies, cell media were removed and fresh serum-free media was given to the cells in the upper compartment, while to lower compartment contained full serum-containing medium. For Beas-2B cells, the lower compartment was also supplemented with 20 ng/ml IL-6. Cell migration was measured fluorometrically after 12 h, by fixing the cells, staining with PI and using the imaging shield which only allows light from the original gel plug-covered area to be measured. For cell invasion, the assay procedure was performed in accordance with the manufacturer’s instructions.
All data are expressed as the mean ± standard deviation (SD). For all experiments, any statistical significance between a single condition and untreated control cells were analyzed using one-way ANOVA followed by a Dunnett post hoc test using Graphpad 6.
Gustafson HH, Holt-Casper D, Grainger DW, Ghandehari H. Nanoparticle uptake: the phagocyte problem. Nano Today. 2015;10(4):487–510.
Teodoro JS, Silva R, Varela AT, Duarte FV, Rolo AP, Hussain S, Palmeira CM. Low-dose, subchronic exposure to silver nanoparticles causes mitochondrial alterations in Sprague-Dawley rats. Nanomedicine. 2016;11(11):1359–75.
Webster CA, Di Silvio D, Devarajan A, Bigini P, Micotti E, Giudice C, Salmona M, Wheeler GN, Sherwood V, Bombelli FB. An early developmental vertebrate model for nanomaterial safety: bridging cell-based and mammalian toxicity assessment. Nanomedicine. 2016;11(6):643–56.
Lorscheidt S, Lamprecht A. Safety assessment of nanoparticles for drug delivery by means of classic in vitro assays and beyond. Expert Opin Drug Deliv. 2016;13(11):1545–58.
Manshian BB, Munck S, Agostinis P, Himmelreich U, Soenen SJ. High content analysis at single cell level identifies different cellular responses dependent on nanomaterial concentrations. Sci Rep. 2015;5:13890.
Manshian BB, Pfeiffer C, Pelaz B, Heimerl T, Gallego M, Moller M, del Pino P, Himmelreich U, Parak WJ, Soenen SJ. High-content imaging and gene expression approaches to unravel the effect of surface functionality on cellular interactions of silver nanoparticles. ACS Nano. 2015;9(10):10431–44.
Li XB, Zhang CC, Bian Q, Gao N, Zhang X, Meng QT, Wu SS, Wang SZ, Xia YK, Chen R. Integrative functional transcriptomic analyses implicate specific molecular pathways in pulmonary toxicity from exposure to aluminum oxide nanoparticles. Nanotoxicology. 2016;10(7):957–69.
Matysiak M, Kapka-Skrzypczak L, Brzoska K, Gutleb AC, Kruszewski M. Proteomic approach to nanotoxicity. J Proteom. 2016;137:35–44.
Oh E, Liu R, Nel A, Gemill KB, Bilal M, Cohen Y, Medintz IL. Meta-analysis of cellular toxicity for cadmium-containing quantum dots. Nat Nanotechnol. 2016;11(5):479–86.
Sun BB, Pokhrel S, Dunphy DR, Zhang HY, Ji ZX, Wang X, Wang MY, Liao YP, Chang CH, Dong JY, Li RB, Madler L, Brinker CJ, Nel AE, Xia T. Reduction of acute inflammatory effects of fumed silica nanoparticles in the lung by adjusting silanol display through calcination and metal doping. ACS Nano. 2015;9(9):9357–72.
Aruoja V, Pokhrel S, Sihtmae M, Mortimer M, Madler L, Kahru A. Toxicity of 12 metal-based nanoparticles to algae, bacteria and protozoa. Environ Sci Nano. 2015;2(6):630–44.
Pokhrel S, Nel AE, Madler L. Custom-designed nanomaterial libraries for testing metal oxide toxicity. Acc Chem Res. 2013;46(3):32–641.
Zhang HY, Pokhrel S, Ji ZX, Meng H, Wang X, Lin SJ, Chang CH, Li LJ, Li RB, Sun BB, Wang MY, Liao YP, Liu R, Xia T, Madler L, Nel AE. PdO doping tunes band-gap energy levels as well as oxidative stress responses to a Co3O4 p-type semiconductor in cells and the lung. J Am Chem Soc. 2014;136(17):6406–20.
Manshian BB, Moyano DF, Corthout N, Munck S, Himmelreich U, Rotello VM, Soenen SJ. High-content imaging and gene expression analysis to study cell–nanomaterial interactions: the effect of surface hydrophobicity. Biomaterials. 2014;35(37):9941–50.
Peynshaert K, Manshian BB, Joris F, Braeckmans K, De Smedt SC, Demeester J, Soenen SJ. Exploiting intrinsic nanoparticle toxicity: the pros and cons of nanoparticle-induced autophagy in biomedical research. Chem Rev. 2014;114(15):7581–609.
Nel AE, Madler L, Velegol D, Xia T, Hoek EMV, Somasundaran P, Klaessig F, Castranova V, Thompson M. Understanding biophysicochemical interactions at the nano-bio interface. Nat Mater. 2009;8(7):543–57.
Diaz B, Sanchez-Espinel C, Arruebo M, Faro J, de Miguel E, Magadan S, Yague C, Fernandez-Pacheco R, Ibarra MR, Santamaria J, Gonzalez-Fernandez A. Assessing methods for blood cell cytotoxic responses to inorganic nanoparticles and nanoparticle aggregates. Small. 2008;4(11):2025–34.
Soenen SJ, Parak WJ, Rejman J, Manshian B. (Intra)Cellular stability of inorganic nanoparticles: effects on cytotoxicity, particle functionality, and biomedical applications. Chem Rev. 2015;115(5):2109–35.
Xia TA, Zhao Y, Sager T, George S, Pokhrel S, Li N, Schoenfeld D, Meng HA, Lin SJ, Wang X, Wang MY, Ji ZX, Zink JI, Madler L, Castranova V, Lin S, Nel AE. Decreased dissolution of ZnO by iron doping yields nanoparticles with reduced toxicity in the rodent lung and zebrafish embryos. ACS Nano. 2011;5(2):1223–35.
Setyawati MI, Yuan X, Xie JP, Leong DT. The influence of lysosomal stability of silver nanomaterials on their toxicity to human cells. Biomaterials. 2014;35(25):6707–15.
Jeong JK, Gurunathan S, Kang MH, Han JW, Das J, Choi YJ, Kwon DN, Cho SG, Park C, Seo HG, Song H, Kim JH. Hypoxia-mediated autophagic flux inhibits silver nanoparticle-triggered apoptosis in human lung cancer cells. Sci Rep. 2016;6:21688.
Mao BH, Tsai JC, Chen CW, Yan SJ, Wang YJ. Mechanisms of silver nanoparticle-induced toxicity and important role of autophagy. Nanotoxicology. 2016;10(8):1021–40.
Zhang XD, Zhang HQ, Liang X, Zhang JX, Tao W, Zhu XB, Chang DF, Zeng XW, Mei L. Iron oxide nanoparticles induce autophagosome accumulation through multiple mechanisms: lysosome impairment, mitochondrial damage, and ER stress. Mol Pharm. 2016;13(7):2578–87.
Li YB, Zhu HY, Wang SF, Qian XL, Fan JJ, Wang ZY, Song P, Zhang XS, Lu WY, Ju DW. Interplay of oxidative stress and autophagy in PAMAM dendrimers-induced neuronal cell death. Theranostics. 2015;5(12):1363–77.
Jeon YM, Lee MY. Airborne nanoparticles (PM0.1) induce autophagic cell death of human neuronal cells. J Appl Toxicol. 2016;36(10):1332–42.
Kroemer G, Levine B. Autophagic cell death: the story of a misnomer. Nat Rev Mol Cell Biol. 2008;9(12):1004–10.
Codogno P, Mehrpour M, Proikas-Cezanne T. Canonical and non-canonical autophagy: variations on a common theme of self-eating? Nat Rev Mol Cell Biol. 2012;13(1):7–12.
Luo YH, Wu SB, Wei YH, Chen YC, Tsai MH, Ho CC, Lin SY, Yang CS, Lin PP. Cadmium-based quantum dot induced autophagy formation for cell survival via oxidative stress. Chem Res Toxicol. 2013;26(5):662–73.
Zhang XC, Yin HQ, Li ZG, Zhang T, Yang Z. Nano-TiO2 induces autophagy to protect against cell death through antioxidative mechanism in podocytes. Cell Biol Toxicol. 2016;32(6):513–27.
Huang DT, Zhou HL, Gao JH. Nanoparticles modulate autophagic effect in a dispersity-dependent manner. Sci Rep. 2015;5:14361.
Ma XW, Wu YY, Jin SB, Tian Y, Zhang XN, Zhao YL, Yu L, Liang XJ. Gold nanoparticles induce autophagosome accumulation through size-dependent nanoparticle uptake and lysosome impairment. ACS Nano. 2011;5(11):8629–39.
Takenaka S, Moller W, Semmler-Behnke M, Karg E, Wenk A, Schmid O, Stoeger T, Jennen L, Aichler M, Walch A, Pokhrel S, Madler L, Eickelberg O, Kreyling WG. Efficient internalization and intracellular translocation of inhaled gold nanoparticles in rat alveolar macrophages. Nanomedicine. 2012;7(6):855–65.
Moller W, Gibson N, Geiser M, Pokhrel S, Wenk A, Takenaka S, Schmid O, Bulgheroni A, Simonelli F, Kozempel J, Holzwarth U, Wigge C, Eigeldinger-Berthou S, Madler L, Kreyling WG. Gold nanoparticle aerosols for rodent inhalation and translocation studies. J Nanopart Res. 2013;15(4):1574.
Lehman SE, Morris AS, Mueller PS, Salem AK, Grassian VH, Larsen SC. Silica nanoparticle-generated ROS as a predictor of cellular toxicity: mechanistic insights and safety by design. Environ Sci Nano. 2016;3(1):56–66.
Schlinkert P, Casals E, Boyles M, Tischler U, Hornig E, Tran N, Zhao JY, Himly M, Riediker M, Oostingh GJ, Puntes V, Duschl A. The oxidative potential of differently charged silver and gold nanoparticles on three human lung epithelial cell types. J Nanobiotechnol. 2015;13:1. https://doi.org/10.1186/s12951-014-0062-4.
Knopp A, Thierfelder S, Doepner B, Benndorf K. Mitochondria are the main ATP source for a cytosolic pool controlling the activity of ATP-sensitive K+ channels in mouse cardiac myocytes. Cardiovasc Res. 2001;52(2):236–45.
Chaudhary AK, Yadav N, Bhat TA, O’Malley J, Kumar S, Chandra D. A potential role of X-linked inhibitor of apoptosis protein in mitochondrial membrane permeabilization and its implication in cancer therapy. Drug Discov Today. 2016;21(1):38–47.
Goldman SJ, Taylor R, Zhang Y, Jin SK. Autophagy and the degradation of mitochondria. Mitochondrion. 2010;10(4):309–15.
George S, Pokhrel S, Ji ZX, Henderson BL, Xia T, Li LJ, Zink JI, Nel AE, Madler L. Role of Fe doping in tuning the band gap of TiO2 for the photo-oxidation-induced cytotoxicity paradigm. J Am Chem Soc. 2011;133(29):11270–8.
Pham AL, Sedlak DL, Doyle FM. Dissolution of mesoporous silica supports in aqueous solutions: implications for mesoporous silica-based water treatment processes. Appl Catal B. 2012;126:258–64.
Kenzaoui BH, Bernasconi CC, Guney-Ayra S, Juillerat-Jeanneret L. Induction of oxidative stress, lysosome activation and autophagy by nanoparticles in human brain-derived endothelial cells. Biochem J. 2012;441:813–21.
Neibert KD, Maysinger D. Mechanisms of cellular adaptation to quantum dots—the role of glutathione and transcription factor EB. Nanotoxicology. 2012;6(3):249–62.
Xu Q, Liu W, Liu XL, Liu WW, Wang HJ, Yao GD, Zang LH, Hayashi T, Tashiro S, Onodera S, Ikejima T. Silibinin negatively contributes to primary cilia length via autophagy regulated by histone deacetylase 6 in confluent mouse embryo fibroblast 3T3-L1 cells. Mol Cell Biochem. 2016;420(1–2):53–63.
Soenen SJH, Nuytten N, De Meyer SF, De Smedt SC, De Cuyper M. High intracellular iron oxide nanoparticle concentrations affect cellular cytoskeleton and focal adhesion kinase-mediated signaling. Small. 2010;6(7):832–42.
Soenen SJ, Manshian B, Montenegro JM, Amin F, Meermann B, Thiron T, Cornelissen M, Vanhaecke F, Doak S, Parak WJ, De Smedt S, Braeckmans K. Cytotoxic effects of gold nanoparticles: a multiparametric study. ACS Nano. 2012;6(7):5767–83.
Del Pino P, Yang F, Pelaz B, Zhang Q, Kantner K, Hartmann R, Martinez de Baroja N, Gallego M, Möller M, Manshian BB, Soenen SJ, Riedel R, Hampp N, Parak WJ. Basic physicochemical properties of polyethylene glycol coated gold nanoparticles that determine their interaction with cells. Angew Chem Int Ed Engl. 2016;55(18):5483–7.
Pérez-Hernández M, Moros M, Stepien G, Del Pino P, Menao S, de Las Heras M, Arias M, Mitchell SG, Pelaz B, Gálvez EM, de la Fuente JM, Pardo J. Multiparametric analysis of anti-proliferative and apoptotic effects of gold nanoprisms on mouse and human primary and transformed cells, biodistribution and toxicity in vivo. Part Fibre Toxicol. 2017;14(1):41.
Soenen SJ, Manshian B, Doak SH, De Smedt SC, Braeckmans K. Fluorescent non-porous silica nanoparticles for long-term cell monitoring: cytotoxicity and particle functionality. Acta Biomater. 2013;9(11):9183–93.
Tay CY, Cai PQ, Setyawati MI, Fang WR, Tan LP, Hong CHL, Chen XD, Leong DT. Nanoparticles strengthen intracellular tension and retard cellular migration. Nano Lett. 2014;14(1):83–8.
Schappi JM, Krbanjevic A, Rasenick MM. Tubulin, actin and heterotrimeric G proteins: coordination of signaling and structure. BBA Biomembr. 2014;1838(2):674–81.
Hall JE, Fu W, Schaller MD. Focal adhesion kinase: exploring Fak structure to gain insight into function. Int Rev Cel Mol Biol. 2011;288:185–225.
Berry CC, Wells S, Charles S, Curtis ASG. Dextran and albumin derivatised iron oxide nanoparticles: influence on fibroblasts in vitro. Biomaterials. 2003;24(25):4551–7.
Kostura L, Kraitchman DL, Mackay AM, Pittenger MF, Bulte JWM. Feridex labeling of mesenchymal stem cells inhibits chondrogenesis but not adipogenesis or osteogenesis. NMR Biomed. 2004;17(7):513–7.
Roeder E, Henrionnet C, Goebel JC, Gambier N, Beuf O, Grenier D, Chen BL, Vuissoz PA, Gillet P, Pinzano A. Dose–response of superparamagnetic iron oxide labeling on mesenchymal stem cells chondrogenic differentiation: a multi-scale in vitro study. PLoS ONE. 2014;9(5):e98451. https://doi.org/10.1371/journal.pone.0098451.
Chen YC, Hsiao JK, Liu HM, Lai IY, Yao M, Hsu SC, Ko BS, Chen YC, Yang CS, Huang DM. The inhibitory effect of superparamagnetic iron oxide nanoparticle (Ferucarbotran) on osteogenic differentiation and its signaling mechanism in human mesenchymal stem cells. Toxicol Appl Pharmacol. 2010;245(2):272–9.
Balakumaran A, Pawelczyk E, Ren JQ, Sworder B, Chaudhry A, Sabatino M, Stroncek D, Frank JA, Robey PG. Superparamagnetic iron oxide nanoparticles labeling of bone marrow stromal (mesenchymal) cells does not affect their “stemness’’. PLoS ONE. 2010;5(7):e11462. https://doi.org/10.1371/journal.pone.0011462.
Ramirez-Peinado S, Leon-Annicchiarico CL, Galindo-Moreno J, Iurlaro R, Caro-Maldonado A, Prehn JHM, Ryan KM, Munoz-Pinedo C. Glucose-starved cells do not engage in prosurvival autophagy. J Biol Chem. 2013;288(42):30387–98.
Nobes CD, Hall A. Rho, Rac, and Cdc42 Gtpases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia. Cell. 1995;81(1):53–62.
Besson A, Gurian-West M, Schmidt A, Hall A, Roberts JM. p27(Kip1) modulates cell migration through the regulation of RhoA activation. Gene Dev. 2004;18(8):862–76.
Sander EE, ten Klooster JP, van Delft S, van der Kammen RA, Collard JG. Rac downregulates Rho activity: reciprocal balance between both GTPases determines cellular morphology and migratory behavior. J Cell Biol. 1999;147(5):1009–21.
Turcotte S, Desrosiers RR, Beliveau R. HIF-1 alpha mRNA and protein upregulation involves Rho GTPase expression during hypoxia in renal cell carcinoma. J Cell Sci. 2003;116(11):2247–60.
Wang WC, Kuo CY, Tzang BS, Chen HM, Kao SH. IL-6 augmented motility of airway epithelial cell BEAS-2B via Akt/GSK-3 beta signaling pathway. J Cell Biochem. 2012;113(11):3567–75.
Haasters F, Docheva D, Gassner C, Popov C, Bocker W, Mutschler W, Schieker M, Prall WC. Mesenchymal stem cells from osteoporotic patients reveal reduced migration and invasion upon stimulation with BMP-2 or BMP-7. Biochem Biophys Res Commun. 2014;452(1):118–23.
Supino R, Petrangolini G, Pratesi G, Tortoreto M, Favini E, Dal Bo L, Casalini P, Radaelli E, Croce AC, Bottiroli G, Misiano P, Farina C, Zunino F. Antimetastatic effect of a small-molecule vacuolar H+-ATPase inhibitor in in vitro and in vivo preclinical studies. J Pharmacol Exp Ther. 2008;324(1):15–22.
The study was designed by SJS and BBM. Nanoparticle characterization was performed and analysed by SP and LM. Biological tests were performed and data analyzed by BBM and SJS. All authors contributed to writing the manuscript. All authors read and approved the final manuscript.
This work was supported by the European Research Council (S.J.S., NanOnc, ERC-2017-StG 757398), FWO-Vlaanderen (KAN to B.B.M. and S.J.S.), the Flemish agency for Innovation through Science and Technology (IWT SBO NanoComit to U.H.) and by the U.S. Public Health Service Grant, R01 ES016746, with leveraged support from the National Science Foundation and the Environmental Protection Agency under Cooperative Agreement Number DBI 0830117 and 1266377, EU Commission (MODERN, Contract no. 309314).
The authors declare that they have no competing interests.
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The datasets used and/or analysed during the current study are available from the corresponding author on reasonable request.
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This work was supported by the European Research Council (S.J.S., NanOnc, ERC-2017-StG 757398), FWO-Vlaanderen (KAN to B.B.M. and S.J.S.), and by the U.S. Public Health Service Grant, R01 ES016746, with leveraged support from the National Science Foundation and the Environmental Protection Agency under Cooperative Agreement Number DBI 0830117 and 1266377, EU Commission (MODERN, Contract no. 309314).
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Manshian, B.B., Pokhrel, S., Mädler, L. et al. The impact of nanoparticle-driven lysosomal alkalinization on cellular functionality. J Nanobiotechnol 16, 85 (2018). https://doi.org/10.1186/s12951-018-0413-7
- Gold nanoparticles
- Silicon dioxide nanoparticles