- Open Access
Novel self-assembling conjugates as vectors for agrochemical delivery
- Pavani P. Nadiminti†1Email author,
- Qingtao Liu†2, 3,
- Lavanya K. Vanjari1,
- Yao D. Dong2,
- Ben J. Boyd2, 3 and
- David M. Cahill1
© The Author(s) 2018
- Received: 10 September 2018
- Accepted: 11 November 2018
- Published: 21 November 2018
Modern agricultural practises rely on surfactant-based spray applications to eliminate weeds in crops. The wide spread and indiscriminate use of surfactants may result in a number of deleterious effects that are not limited to impacts on the crop and surrounding farm eco-system but include effects on human health. To provide a safer alternative to the use of surfactant-based formulations, we have synthesised a novel, self-assembling herbicide conjugate for the delivery of a broad leaf herbicide, picloram.
The synthesized self-assembling amphiphile–picloram (SAP) conjugate has three extending arms: a lipophilic lauryl chain, a hydrophilic polyethylene glycol chain and the amphiphobic agrochemical active picloram. We propose that the SAP conjugate maintains its colloidal stability by quickly transitioning between micellar and inverse micellar phases in hydrophilic and lipophilic environments respectively. The SAP conjugate provides the advantage of a phase structure that enables enhanced interaction with the hydrophobic epicuticular wax surface of the leaf. We have investigated the herbicidal efficiency of the SAP conjugate compared against that of commercial picloram formulations using the model plant Arabidopsis thaliana and found that when tested at agriculturally relevant doses between 0.58 and 11.70 mM a dose-dependent herbicidal effect with comparable kill rates was evident.
Though self-assembling drug carriers are not new to the pharmaceutical industry their use for the delivery of agrochemicals shows great promise but is largely unexplored. We have shown that SAP may be used as an alternative to current surfactant-based agrochemical formulations and has the potential to shift present practises towards a more sustainable approach.
- Arabidopsis thaliana
Common agricultural practises aimed at improving plant health and crop yield are heavily dependent on the use of surfactant-based agrochemical formulations. Such formulations are known for their off-target toxicity , lead to the over-use of chemicals  and may result in crop phytotoxicity [3, 4] ultimately resulting in yield loses. In addition, traditional agrochemicals may significantly contribute to pollution of the environment and can be deleterious to human health [5, 6]. To overcome these unintended impacts new strategies for agrochemical delivery are under constant exploration [7, 8]. One such innovative strategy is the use of nanotechnology, which has opened new vistas for the delivery of agrochemicals to plants . Unlike traditional agrochemicals, research with the model plant species, Arabidopsis thaliana, showed that mesoporous silica nanoparticles (MSNs), can deliver the phytohormone and disease resistance inducer, salicylic acid, in-planta only when redox stress is high . Also, MSNs were shown to be able to deliver the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) to control the growth of model weeds while simultaneously reducing soil contamination . In our recent work, lyotropic liquid crystalline (LC) phases were shown in laboratory and field studies to minimise crop phytotoxicity while efficiently delivering 2,4-D to eliminate weeds .
LC systems have been shown to have high pharmaceutical value for their ability to deliver a range of molecules such as curcumin , chlorhexidine  and insulin . LC systems encompass a number of phases such as lamellar phases, hexagonal phases and inverse bicontinuous cubic phases. The latter phases can simply be formed by dispersion in water of polar lipids such as phytantriol, glyceryl monooleate and glyceryl monoelaidin in the presence of a stabiliser . Such LC systems have recently been of interest also for the delivery of agrochemical actives to plants. LC systems in inverse bicontinuous cubic phase structure can interact with hydrophobic plant surfaces, prevent crop phytotoxicity and reduce off target toxicity effects [12, 17]. In field trials the practical use of a surfactant free LC system for the delivery of 2,4-D to kill wild radish (Raphanus raphanistrum) in a wheat (Triticum aestivum) crop was demonstrated . However, their success was dependent on the amphiphobic agrochemical in use that often hampers the stability of the formulation [18–20].
Agrochemical industries employ several strategies to improve the stability of oil in water emulsions, for example, by encapsulation of the active ingredient , alkoxylation of the hydrophobic active ingredient  and the use of solid dispersing agents to stabilise the emulsion containing lipophilic actives . In a recent report we demonstrated the use of an agrochemical conjugate for the formation of a stable self-assembling LC carrier system . The linear-picloram conjugate was prepared by covalently bonding agrochemical actives such as picloram or 2,4-D to a lipid, which, when exposed to water in the presence of a pluronic stabiliser, formed a self-assembling LC system. The use of such formulation is restricted by the presence of amphiphobic actives that limit their encapsulation into the LC system and reduces the colloidal stability of the formulation . The disadvantages of such systems presents us with the need for the development of an ecologically safe, self-assembling agrochemical formulation that can not only form a stable carrier emulsion but also can efficiently deliver the active to target plants.
The use of self-assembling liquid crystalline materials for the delivery of pharmaceutical actives is well described but has never been explored for the delivery of agrochemical actives . We therefore, synthesized a novel self-assembling amphiphile–picloram (SAP) conjugate with three extending arms consisting of a lipophilic lauryl chain, hydrophilic polyethylene glycol (PEG) chain and an amphiphobic agrochemical active, (picloram in this case). Due to the presence of the extending arms, the SAP conjugate formed a colloidally stable formulation in the absence of surfactants, which enabled the conjugate to better interact with hydrophobic plant surfaces. To test the herbicidal efficacy of the formulation, the model plant species, A. thaliana, was treated with the SAP conjugate at agriculturally relevant doses. The herbicidal effect of the SAP formulation was found to be equal to that of surfactant-based picloram formulations and therefore has considerable potential as an alternative delivery system for agrochemicals.
Picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid) and picloram-oleyl (PO) were the gift of Nufarm Ltd. (Laverton, Vic, Australia), O-(6-chlorobenzotriazol-1-yl)-N,N,N′,N′-tetramethyluronium hexafluorophosphate (HCTU), triisopropylamine, methoxypolyethylene glycol 350 (Me-EO7–OH), 1-bromododecane, 18-crown-6, sodium hydroxide and potassium carbonate anhydrous were purchased from Sigma-Aldrich (St. Louis, MO, USA). p-Toluenesulfonyl chloride (Ots-Cl) and 3,5-dihydroxybenzyl alcohol were purchased from TCL Co. Ltd. (Chuo-ku, Tokyo, Japan). Diethyl ether, chloroform, methanol, acetonitrile and magnesium sulphate anhydrous were purchased from Merck Pty. Ltd. (Kilsyth, Vic, Australia).
Preparation of 3-(dodecyloxy)-5-(hydroxymethyl)phenol (2)
Preparation of Ots-EO7-Me (4)
20 mM Me-EO7–OH (3) solution in 8 mL tetrahydrofuran (THF) and 24 mmol NaOH aqueous solution (10% w/w) were mixed under continuous stirring in a 100 mL round-bottom flask and placed in an ice bath. While constantly, stirring 8 mL THF solution and 22 mM p-toluenesulfonyl chloride was slowly added to the round-bottom flask and stirred for another 2 h at room temperature. After transferring the reaction to 100 mL milli-Q water, the mixture was extracted thrice with 40 mL chloroform and was dried with 5 g anhydrous magnesium sulfate. The product was then filtered, to remove magnesium sulfate and dried to obtain a crude product. The crude product was further purified via column chromatography using a mixture of chloroform (90% v/v) and methanol (10% v/v) as the eluent. The purified product was a colourless oil (yield was 97%) (Fig. 1) (Additional file 2).
Preparation of 3-(dodecyloxy)-5-(EO7)phenol (5)
0.2 mM Ots-EO7-Me (4), 0.24 mM 3-(dodecyloxy)-5-(hydroxymethyl)phenol (2) and 0.98 g of K2CO3 (0.7 mM) were dissolved in 100 mL DMF in a 250 mL round-bottom flask equipped with a magnetic bar and refluxed at 120 °C for eight hours. To obtain a crude product, the reaction mixture was filtered, to remove insoluble inorganic salts and was dried by reduced pressure distillation. The crude product was further purified by passing it through a chromatography column and was eluted with chloroform (95% v/v) and methanol (5% v/v) to obtain a pure product that was a dark yellow oil (yield is 57%) (Fig. 1) (Additional file 3).
Preparation of 3-(dodecyloxy)-5-(EO7)benzyl-PIC (6)
0.48 g picloram (2 mM) was suspended in 70 mL acetonitrile in a 250 mL round-bottom flask equipped with a magnetic bar, then 1 g HCTU powder (2.4 mM) was added to the picloram suspension and stirred for 10 min. To this and at 10 min intervals, 1 mM of component-5 in 10 mL acetonitrile and 7.2 mM triisopropylamine (1.4 mL) were added with stirring overnight. The reaction solution was then dried to remove acetonitrile by rotary vacuum evaporation. The dried reaction mixture was extracted thrice with 40 mL diethyl ether. The combined diethyl ether extract was further dried to obtain a crude product that was passed through a chromatography column and then eluted using chloroform. The desired final product SAP conjugate, was a yellow oil (yield is 52%) (Fig. 1) (Additional files 4 and 5).
The particle size of self-assembling amphiphile–picloram (SAP) conjugate in water
Particle size of the self-assembling amphiphile–picloram (SAP) conjugate was analysed at different concentrations in water using a zetasizer (Nano-ZS, Malvern Instruments, Malvern, UK) at 25 °C against the viscosity of pure water. Automated settings were used to take size measurements in low-volume cuvettes.
The critical micelle concentration of SAP conjugate in water
The critical micelle concentration (CMC) of SAP conjugate in water was measured using a fluorescent probe method . For this, aqueous solutions of pyrene and SAP conjugate were prepared where the concentration of pyrene was kept constant at 2 µM while that of the SAP conjugate ranged between 0 and 1.2 mM. The fluorescence emission intensity of pyrene in the samples at 370 nm (I1) and 380 nm (I3), was recorded using a Perkin Elmer Enspire® (PerkinElmer Australia, Vic, Australia) multimode plate reader (excitation wavelength was at 330 nm). To obtain the CMC, the ratio of fluorescence emission intensity at I1 and I3 was plotted against the concentration of SAP conjugate, where the inflection point represents CMC.
The water and oil contact angle of SAP conjugate cast membrane
The water and oil contact angle (CA) of a cast film of the SAP conjugate was measured using a goniometer (KSV CAM 101, Biolin Scientific AB, Västra Frölunda, Sweden).
The morphology of SAP conjugate in water
To investigate the internal nanostructure of the SAP conjugate in water transmission electron microscopy (TEM) was performed. For this, a dispersion of SAP conjugate prepared in water was sonicated for 30 min and stored overnight. A ten-µl drop of the SAP conjugate aqueous solution was placed on a copper grid (ProSciTech, Qld, Australia), and allowed to dry at room temperature. TEM images were then collected of the grid using a JEOL 2100 TEM (JOEL Ltd., Tokyo, Japan) operating at 200 kV.
Plant growth and maintenance
To test the herbicidal effect of various formulations, 3-week-old, A. thaliana ecotype Col-0 plants were used. Seeds were surface sterilised as previously described and were transferred to 90 mm Petri plates containing Murashige and Skoog basal medium (Sigma-Aldrich, Sydney, NSW, Australia) containing 30% w/v sucrose, 0.8% w/v bacteriological agar and adjusted to pH 5.7 . The seeds within the Petri plates were stratified at 4 °C for 48 h and the plates then moved to a growth cabinet (Thermoline Scientific, Wetherill Park, NSW, Australia) for 14 days, under the conditions previously described .
Determination of herbicidal effect of picloram
To mimic the herbicidal constituents that are routinely used in agricultural situations, picloram, empimin (an anionic sodium di(2-ethylhexyl) sulfosuccinate), empigen (a cationic and amphoteric betaine C12 − C14 alkyl dimethyl) (all gifts of Nufarm Australia Limited, Vic, Australia) and pluronic F127 (a nonionic polyoxyethylene–polyoxypropylene block co-polymer, Sigma-Aldrich, Australia) were diluted in water to obtain different formulations. A series of range finding experiments were carried out prior to the collection of data from duplicated experiments for all of the following treatment combinations. The formulations tested contained picloram at agriculturally relevant concentrations in the range 4.14 × 10−5 mM [1.0 × 10−6 % (w/v)] − 4.14 × 10−1 mM [1.0 × 10−2 % (w/v)] and mixed with one of the commercial surfactants empimin, empigen or pluronic F127 at 0.01% v/v . To obtain a uniform coverage of leaves the formulations were individually spray-applied using a hand held atomiser (New directions, Sydney, NSW, Australia) to the adaxial surface of leaves of three A. thaliana plants per treatment. The plants were treated on a bench within the laboratory at room temperature and then left to dry (approximately 2 h) and were then transferred to a growth cabinet for observations and measurement of any herbicidal effect. Each plant was imaged three and seven days after spray application and assessed against control plants treated either with distilled water or with the corresponding surfactant formulation (n = 3 for each replicate) . Commercial statistical software (IBM SPSS Version 19; IBM Australia Ltd., St Leonards NSW, Australia) was used to perform Duncan’s posthoc test on the normally distributed data.
Herbicidal effect of the SAP conjugate
To test the efficiency of the SAP conjugate, it was diluted to agriculturally relevant doses in water to final concentrations of 0.585 mM [0.05% (w/v)], 1.17 mM [0.1% (w/v)], 2.34 mM [0.2% (w/v)], 5.85 mM [0.5% (w/v)] and 11.70 mM [1.0% (w/v)] and spray applied to A. thaliana as described above. Water treated controls were set up; picloram and picloram oleyl (PO) were simultaneously made in water to match the concentrations of the conjugate and were similarly sprayed on to A. thaliana plants [12, 17]. Blind assessment is a widely accepted and robust procedure for the assessment of phytotoxic effect induced by herbicidal spray applications . For our work, three independent blind assessors separately ranked all the photographs collected for plants after day three and seven spray treatments on a scale of zero to five (n = 3 for each replicate). As the ranked data was uniformly distributed, it was subjected to Duncan’s posthoc testing (IBM SPSS Version 19, NSW, Australia) and the experiments were performed in triplicate.
The colloidal property of the SAP conjugate in water
The contact angle (CA) measurement of the cast film of the SAP conjugate indicated that the SAP conjugate had excellent amphiphilicity and quickly adjusted to a new environment. For water, the CA of the SAP conjugate decreased from 21.8 ± 2.6° to 3.7 ± 1.8° in 4 min, indicating that the SAP conjugate had good hydrophilicity and capability to form a colloidal-stable dispersion in water. For oil, the CA was close to zero degrees demonstrating the super-lipophilic nature of SAP formulations and a potential to adsorb and interact with the hydrophobic wax layers on a leaf surface.
Defining the phytotoxicity effect
Effect of surfactant-based picloram delivery to A. thaliana plants
Delivery of amphiphile–picloram (SAP) conjugate to A. thaliana
Based on the minimal dose of picloram required to kill A. thaliana plants the experiments related to SAP conjugate application used concentrations above 0.414 (4.14 × 10−1) mM. Before the spray application of the amphiphile–picloram (SAP) conjugate to the plants, its solubility was tested by mixing the SAP conjugate in water at concentrations of 0.585 (or 5.85 × 10−1) mM [equal to 0.05% (w/v)], 1.17 mM [0.1% (w/v)], 2.34 mM [0.2% (w/v)], 5.85 mM [0.5% (w/v)] and 11.70 mM [1.0% (w/v)]. The SAP conjugate formed an emulsion at all the concentrations tested while for picloram-oleyl (PO) and picloram (alone), a precipitate formed at the bottom of the tube at the same concentrations of that for the SAP conjugate (Additional file 9).
To assess the herbicidal efficacy, the SAP conjugate was mixed in water and spray-applied to 3-week-old A. thaliana plants (Additional file 10). With an increase in the concentration of spray-applied SAP to the plants, a clear dose dependency of the herbicidal effect was observed after 3 days (Additional file 10). For the spray application of SAP at a concentration of 0.58 mM the plants were observed to have a strong epinastic response (Additional file 10: Figure S10b), which further extended to petiole curling with an increase in applied SAP conjugate concentration to 1.17 mM (Additional file 10: Figure S10c). With a progressive increase of SAP conjugate concentration to 2.34 mM (Additional file 10: Figure S10d) curled leaves and petioles twisted around the axis of the plant which became more prominent with further increases in the concentration of applied SAP conjugate to 5.85 mM and 11.70 mM (Additional file 10: Figure S10e–f). PO application on the contrary, did not have the same phytotoxicity effect as that of SAP, when applied at the same concentrations. The leaf curling observed for PO spray applications was limited to the lamina, irrespective of the concentration of the PO applied (Additional file 10: Figure S10g–i). Picloram (in water) spray applications showed a consistent and strong herbicidal effect that was similar to the effect elicited by SAP conjugate applications. Unlike other treatments, picloram induced herbicidal effect on the plants was characterised by the presence of chlorosis even when applied at the lowest concentration of 0.58 mM and severe tissue necrosis was observed at high concentrations of 5.85 mM and 11.70 mM (Additional file 10: Figure S10m–r).
Quantification of the herbicidal effects of SAP conjugate spray applications
Phytotoxicity observations at 7 days after spray application of SAP conjugate, PO and picloram were broadly consistent with the day three observations (Fig. 9b). There was a dose-dependent trend of increasing herbicidal effect for all concentrations of SAP conjugate tested and at 5.85 mM and above, the effect was the same as that induced by picloram (in water) alone (Fig. 9b). For PO at both time points, there was not a clear dose-dependent response. The herbicidal effect elicited by picloram (in water) application was the same for all the tested concentrations (Fig. 9b).
The use of self-assembling lipids for the delivery of therapeutics such as antibiotics  and anti-cancer drugs  is well known in the pharmaceutical industry but their use for the delivery of agrochemical actives such as herbicides, has not been examined. Here, we have described the synthesis of a novel self-assembling amphiphile–picloram conjugate for the specific delivery of agrochemicals that aligns with the drive for green chemistry and sustainable agricultural practices. We have shown that when a plant such as A. thaliana grown in vitro was sprayed with agriculturally relevant concentrations of the SAP conjugate containing the herbicide picloram that the nanoformulation was as equally effective for killing target plants as current surfactant-based formulations. This approach to agrochemical delivery has several clear advantages over surfactant-based delivery that include the prevention of crop and off target toxicity, reduction in chemical load and use of less water which, together, benefits the environment and has the potential to increase crop productivity. The three radiating arms that formed the SAP conjugate were the key features of this nanomaterial that enabled the conjugate to form a stable emulsion in water and, when applied, to interact directly with the target hydrophobic plant leaf surface.
Picloram has a pKa value of 2.30 and is poorly soluble in water at 430 mg per litre . Surfactants and adjuvants are therefore tank mixed to influence the physicochemical properties of the agrochemical active and thereby enhance the uptake of the active by the plants . Surfactants (including pluronic stabilisers) have been described several times as the cause of irreversible damage to plants that ultimately lead to yield loss but surfactants are also toxic to the environment and are detrimental to human health . To overcome these disadvantages associated with surfactant-based agrochemical delivery we have earlier proposed the use of LC systems for the safe delivery of agrochemicals to plants . Phase structure is often sterically stabilised by the use of pluronic stabilisers and the physiochemical properties of the LC systems are the critical rate limiting factors that influence their interaction with hydrophobic plant surfaces [33, 34]. Similarly, linear agrochemical conjugates synthesised by conjugating picloram to lipids (phytantriol and glyceryl monooleate) and that attain higher loading efficiency of picloram are limited in their use as an agrochemical active due to the presence of a stearic stabiliser .
When the aqueous formulation of the self-assembling conjugate was spray applied to hydrophobic leaf surfaces, the extending arms of the SAP conjugate enabled its quick transition upon the evaporation of the water. For the activity of the SAP conjugate, it is inaccurate to assume that herbicidal activity will occur after the disassociation of picloram from the conjugate. We predict that the entire SAP conjugate is adsorbed onto the plant cuticular surface. Picloram, because of its strong covalent bond with 3-(dodecyloxy)-5-(EO7)phenol (component 5) is very unlikely to freely disassociate from the conjugate. After adsorption the SAP conjugate acts like a lipophilic non-electrolyte in that it diffuses through the amorphous regions of the cuticle to elicit the herbicidal effect . The herbicidal effect is thus a constitutive effect of the exposed active site of the picloram molecule that is undisturbed during the covalent bonding with 3-(dodecyloxy)-5-(EO7) phenol.
Arabidopsis thaliana plants elicited a dose-dependent herbicidal effect when treated with increasing doses of picloram that contained equal amounts of either of the surfactants empimin, empigen or F127. To mimic the current agricultural practices, the laboratory experiments included agriculturally relevant doses of picloram and surfactants. Surfactants are conventionally tank mixed with agrochemical actives such as picloram to enhance the herbicidal effect of the active, by influencing the physiochemical properties of both the plant cuticle and the active ingredient [37, 38]. Despite the addition of the surfactants, picloram applications failed to induce a statistically significant herbicidal effect for the range of picloram concentrations that we tested, and within the course of the experiments. Therefore, 4.14 × 10−1 mM was determined to be the minimal effective dose of picloram that completely killed A. thaliana. Matching the surfactant or adjuvant with the active ingredient is a fine art and is necessary for selection of the combination of surfactant and active that enables the active exert its greatest effect.
Picloram like 2,4-D is an amphiphobic phenoxy herbicides (or auxin mimic) that is routinely used to eliminate dicot weeds such as wild radish (Raphanus raphanistrum), wild mustard (Sinapis arvensis) and wild turnip (Myagrum rugosum) in monocot crops such as wheat (Triticum aestivum), barley (Hordeum vulgare) and oats (Avena sativa) . Epinasty, tissue necrosis, chlorosis and plant death characterise the herbicidal effect elicited by phenoxy herbicides . Auxin mimics act by disrupting the hormonal homeostasis and thereby induce tissue necrosis; inducing over-production of ethylene, reactive oxygen species and hydrogen cyanide; causing growth inhibition and reduction in CO2 assimilation and that ultimately leads to plant death [40, 41].
In our laboratory experiments, for all the formulations, a dose range that was agriculturally relevant was tested. It is likely that the diffusion of SAP conjugate is slower than picloram by itself due to its larger size that in turn limits the rate of diffusion of the conjugate into the amorphous regions of the cuticle, that are usually available for the diffusion of lipophilic non-electrolytes . At the tested doses, picloram oleyl failed to exhibit any significant herbicidal effect compared with that shown for SAP conjugate or picloram formulations. The lack of herbicidal effect indicated that alkylation of an amphiphobic agrochemical active, such as picloram, does not enhance efficacy.
It was recently shown that agrochemicals, such as pesticides, contribute to the largest fraction of volatile organic compound sources that not only damage the environment but also increasingly impact human health . In addition, surfactant based agrochemicals irreversibly damage plant cuticular surfaces [3, 42]. To overcome the deleterious effects posed by traditional agrochemical formulations we propose the use of surfactant-free conjugate-based self-assembly formulations. We expect that the interactions of self-assembling lipid-based conjugates with the hydrophobic surface of crop plant leaves will, as has been found previously for at least one other lipid based nano-formulation, not damage the epicuticular wax micromorphology therefore reducing both phytotoxicity and potential yield loss . Use of self-assembling formulations can also prevent off-target environmental impacts by minimising off-target toxicity, require less water for spray application and the amount of agrochemical runoff will be reduced . Key to understanding how agrochemical formulations penetrate into plant cells is in understanding the ultrastructure of the cuticle on plant surfaces which is characterised by the presence of cuticular waxes that are interspersed in a cutin matrix [43, 44]. Here, we have used the model plant A. thaliana in our proof of concept study but clearly agriculturally relevant crop and weed species must now be examined for their interactions with self-assembling conjugates and with a variety of actives. We expect that self-assembling conjugates will find broad use in agriculture for the delivery of actives such as herbicides, pesticides and fungicides and that their use will strengthen existing practises for sustainable agriculture.
PPN performed the experiments on plants and wrote the first draft of the manuscript. QL prepared and characterized the nanomaterial and contributed to writing the manuscript. LKV performed the surfactant based picloram delivery experiments. YDD designed the nanoparticles and assisted with their synthesis. BJB and DMC conceived, and had oversight of the project, secured funding and assisted writing the manuscript. All authors read and approved the final manuscript.
The research was supported by an Australian Research Council Linkage Project Grant (Project Number LP130100903) and a collaboration between Deakin University, Monash University and the Victorian Chemical Company, Coolaroo, Victoria, Australia.
The authors declare that they have no competing interests.
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Supported by the Australian Research Council (LP130100903). The Australian Research Council’s (ARC) involvement is limited to financial support that allows the researchers to perform the intended research work and they do not have any involvement in the research activities undertaken by the researchers. A final report of the research outcomes is provided to the ARC on completion of the project.
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