- Open Access
Alleviating the hypoxic tumor microenvironment with MnO2-coated CeO2 nanoplatform for magnetic resonance imaging guided radiotherapy
Journal of Nanobiotechnology volume 21, Article number: 90 (2023)
Radiotherapy is a commonly used tool in clinical practice to treat solid tumors. However, due to the unique microenvironment inside the tumor, such as high levels of GSH, overexpressed H2O2 and hypoxia, these factors can seriously affect the effectiveness of radiotherapy.
Therefore, to further improve the efficiency of radiotherapy, a core–shell nanocomposite CeO2–MnO2 is designed as a novel radiosensitizer that can modulate the tumor microenvironment (TME) and thus improve the efficacy of radiation therapy. CeO2–MnO2 can act as a radiosensitizer to enhance X-ray absorption at the tumor site while triggering the response behavior associated with the tumor microenvironment. According to in vivo and in vitro experiments, the nanoparticles aggravate the killing effect on tumor cells by generating large amounts of ROS and disrupting the redox balance. In this process, the outer layer of MnO2 reacts with GSH and H2O2 in the tumor microenvironment to generate ROS and release oxygen, thus alleviating the hypoxic condition in the tumor area. Meanwhile, the manganese ions produced by degradation can enhance T1-weighted magnetic resonance imaging (MRI). In addition, CeO2–MnO2, due to its high atomic number oxide CeO2, releases a large number of electrons under the effect of radiotherapy, which further reacts with intracellular molecules to produce reactive oxygen species and enhances the killing effect on tumor cells, thus having the effect of radiotherapy sensitization. In conclusion, the nanomaterial CeO2–MnO2, as a novel radiosensitizer, greatly improves the efficiency of cancer radiation therapy by improving the lack of oxygen in tumor and responding to the tumor microenvironment, providing an effective strategy for the construction of nanosystem with radiosensitizing function.
In conclusion, the nanomaterial CeO2–MnO2, as a novel radiosensitizer, greatly improves the efficiency of cancer radiation therapy by improving the lack of oxygen in tumor and responding to the tumor microenvironment, providing an effective strategy for the construction of nanosystems with radiosensitizing function.
Cancer is one of the most life-threatening diseases to human health . Researchers have developed various anti-cancer strategies such as chemotherapy, radiotherapy and immunotherapy [2,3,4]. Among them, radiotherapy is a very effective and commonly used method to eliminate tumors . However, the rapid growth of tumors leads to tumor microenvironment characterized by hypoxia, microacidity, high levels of glutathione and hydrogen peroxide [6,7,8], which also makes radiation therapy less effective and creates radiotherapy resistance.
Moreover, in clinical practice, radiotherapy inevitably causes irreversible damage to normal tissues and cells [9,10,11]. Therefore, the development of radiotherapy sensitizers can greatly overcome the shortcomings of conventional radiotherapy and reduce the toxic side effects caused by conventional radiotherapy [12,13,14]. Currently, it has been found that many materials have the ability to enhance the sensitivity of tumor cells to X-rays, such as the small molecule paclitaxel [15,16,17] and metal complexes [18,19,20,21]. However, they are less selective and more toxic to normal cells and tissues. Therefore, there is an urgent need for researchers to develop new efficient and low-toxic radiotherapy sensitizers.
Researchers have studied and developed numerous novel radiotherapy sensitizers, with nanomaterials being particularly prominent [22,23,24]. Nanomaterials usually respond to the tumor microenvironment and can boost the sensitivity of tumor tissue to radiation . They can increase the radiotherapy effect at the lesion site and achieve radiotherapy sensitization. Metal nanoparticles with high atomic number are introduced into tumor tissues and then treated with high-energy radiation to release electrons [26, 27]. The released electrons react with organic molecules or water in cancer cells to produce large amounts of ROS, thus enhancing the effect of radiotherapy [28,29,30]. However, without metal nanoparticles of high atomic number, the effect of radiotherapy sensitization is not satisfactory. As a metal oxide with a high atomic number, CeO2 can enhance the deposition of intracellular radiation and produce a large amount of free radicals to kill tumor cells in the presence of X-rays [31, 32]. At the same time, it has low toxicity to normal tissues and cells, which can overcome the toxic and side effects caused by conventional radiotherapy [33, 34].
Hypoxia is a prominent feature of the tumor microenvironment and has long been considered as a key factor contributing to the tolerance of radiotherapy in solid tumors [35,36,37]. In recent years, researchers have also developed different strategies to alleviate hypoxia within the tumor, such as oxygen delivery to the tumor region [38, 39] and in situ oxygen generation [40,41,42] in the tumor region. However, there is a problem with the strategy of delivering oxygen to the hypoxic region in a tumor due to the uneven distribution of blood vessels within the tumor. To solve the above problem, the high H2O2 concentration in the tumor region has been used to catalyze the in situ generation of oxygen. MnO2 nanomaterials have proven to be a hot spot for researchers who are seeking to catalyze the production of O2 from H2O2 to overcome the problem of tumor hypoxia [43, 44]. Moreover, Mn2+ generated by the reaction between MnO2 and GSH can be used in MRI [45, 46]. Therefore, the radiation therapy effect can be enhanced by making full use of the radiotherapy sensitizing property of CeO2 and the property of MnO2 to improve the hypoxic condition in the tumor area and enhance the radiotherapy effect. Compared to traditional radiotherapy sensitizers, the nanoparticles we proposed have the advantages of high efficiency and low toxicity, high selectivity and guided treatment by MRI.
The goal of this study was to synthesize core–shell CeO2–MnO2 nanoparticles with significant radiosensitizing effect by hydrothermal method , which was found to be effective in killing solid tumors and improving tumor hypoxia. First, the successful synthesis of core–shell CeO2–MnO2 nanoparticles was demonstrated by a series of characterization methods. Subsequently, it was proven in vitro that CeO2–MnO2 has superior performance in catalyzing the generation of O2 from hydrogen peroxide. Finally, using MIHA cells as a normal cell model, the synergistic group of CeO2–MnO2 and X-ray was confirmed to have a significant protective effect on normal cells by MTT assays. HeLa cells were also used as a tumor cell model, and in vivo and in vitro experiments suggested that under X-ray irradiation, CeO2–MnO2 exerted a positive anti-tumor effect by generating massive ROS in the cells, leading to a flip in mitochondrial membrane potential and accelerating apoptosis of tumor cells (Scheme 1). In conclusion, CeO2–MnO2 nanoparticle is a novel, low-toxicity radiosensitizing nanosystem that improves the efficiency of radiation therapy in vivo and in vitro by improving hypoxia, enhancing ROS production and promoting apoptosis of cancer cells.
Results and discussion
Rational design and synthesis of CeO2–MnO2 nanosystem
In this study, we synthesized CeO2–MnO2 nanoparticles through using hydrothermal method (Fig. 1A). The size and shape of the materials were investigated by TEM. Figure 1B showed that CeO2 was a rod-like nanoparticle with a particle size of about 100 nm. MnO2 (Fig. 1C) was a nanoparticle that exhibits a distinct sheet-like shape with a particle size of about 150 nm. It is obvious that CeO2–MnO2 displays a core–shell structure with a size of about 100 nm. Rod-shaped CeO2 nanoparticles were covered with MnO2 nanosheets (Fig. 1D). Based on the EDS elemental analysis (Fig. 1E), the above conclusion can also be verified. Mn and O elements were observed on the surface of CeO2, further verifying the encapsulation of MnO2 on CeO2. The hydration diameters (Additional file 1: Fig. S1) and potential diagrams (Fig. 1G) showed that the average hydration diameter of CeO2 nanoparticles is about 450 nm, and MnO2 is about 120 nm, and the combined CeO2–MnO2 is about 580 nm. CeO2 and MnO2 alone have obvious positive electrical properties, and CeO2–MnO2 exhibits stronger positive electrical properties. Besides, to further evaluate the encapsulation of MnO2 on the CeO2 surface, Raman, UV and XRD analyses were performed. According to the Raman diagram (Fig. 1I), it was observed that CeO2–MnO2 nanoparticles have common peaks with CeO2 and MnO2 at about 460 cm−1 and 670 cm−1, respectively. The presence of CeO2 and MnO2 in CeO2–MnO2 nanoparticles were verified by UV–Vis spectroscopy (Fig. 1H). Also, the results demonstrate the CeO2–MnO2 have the same peaks with CeO2 and MnO2 respectively, corresponding to 123 nm and 399 nm. X-ray diffraction (XRD) patterns showed that the characteristic peaks of CeO2, MnO2 all corresponded to CeO2–MnO2, in accordance with the JCPDS No. 81-2261 of the MnO2 crystal and JCPDS No. 34-0394 of the CeO2 crystal (Fig. 1F). In summary, all results confirm the successful synthesis of CeO2–MnO2.
The ability of CeO2–MnO2 to catalyze hydrogen peroxide, depletion of GSH, rise in ROS concentration
The catalysis of hydrogen peroxide by CeO2–MnO2, the depleted GSH, as well as the rise in ROS induced by radiotherapy are shown in Fig. 2A. Hypoxia leads to the insensitivity of tumor cells to radiotherapy. To verify that CeO2–MnO2 nanoparticles have favorable catalytic properties to produce oxygen from hydrogen peroxide, the rate of hydrogen peroxide scavenging by CeO2–MnO2 was examined in vitro using a hydrogen peroxide kit. The results showed that CeO2–MnO2 had the fastest hydrogen peroxide clearance at a concentration of 100 μg/mL compared to CeO2 and MnO2 alone, with a clearance rate of approximately 60% compared to the control group (Additional file 1: Fig. S2). The rate of H2O2 scavenging by CeO2–MnO2 at different concentrations was also examined, and Fig. 2B showed that the scavenging rate had a significant concentration dependence. To further investigate the performance of the material to catalyze the generation of oxygen from hydrogen peroxide, we monitored the ability to generate oxygen within 15 min by adding different concentrations of H2O2 to the CeO2–MnO2 solution using a dissolved oxygen analyzer. Figure 2C showed that the oxygen content reached a maximum after 5 min, and the amount of O2 produced was dependent on the concentration of H2O2. These results indicate that CeO2–MnO2 has a reasonable ability to catalyze the production of O2 from H2O2. Radiotherapy can lead to the deposition of intracellular energy and the generation of large amounts of reactive oxygen species. These reactive oxygen species can disrupt the redox balance in cells and thus can lead to cellular damage. Therefore, we next explored the overproduction of ROS triggered by CeO2–MnO2 combined with X-ray irradiation. Electron spin resonance (ESR) spectroscopy results confirm that CeO2–MnO2 enhances •OH production, while X-ray (8 Gy) irradiation further increases •OH production (Fig. 2D, F). We also used DCFH-DA and DHE probes to detect ROS and •O2− generated before and after CeO2–MnO2 combined with X-ray (Additional file 1: Fig. S3–S4). Although CeO2–MnO2 was also able to produce ROS in the absence of X-rays, the ROS level increased significantly after combining with X-ray, and it was higher than that of the CeO2 combined X-ray group and MnO2 combined X-ray group. The above results indicate that CeO2–MnO2 combined with X-ray can produce a large amount of ROS. The content of GSH inside the tumor is higher than that of normal cells. Due to the fact that GSH scavenges free radicals to protect cells, the overexpression of GSH can reduce the effects of radiotherapy. Therefore, the responsiveness of CeO2–MnO2 to GSH was investigated. It is shown in Fig. 2G that the characteristic absorption peaks of CeO2–MnO2 in the UV spectrum decreased with the increase of GSH concentration, indicating the reaction of both. Additionally, the color of CeO2–MnO2 solution changed from yellow to colorless as GSH concentration increased, indicating that CeO2–MnO2 consumed GSH. T1-weighted MRI signal may be enhanced by CeO2–MnO2 since it is capable of consuming GSH in TME and generating Mn2+. Therefore, we evaluated the imaging capability of CeO2–MnO2 in vitro. As shown in Fig. 2H, I, the T1-weighted signal intensity of CeO2–MnO2 was significantly enhanced in the presence of GSH. As a result, CeO2–MnO2 is decomposed by GSH to generate Mn2+, which enhances the T1-weighted signal. The above results reveal that CeO2–MnO2 nanoparticles have superior functions of enhancing ROS, catalyzing oxygen generation from hydrogen peroxide.
X-rays stimulate ROS production to enhance the anti-cancer effect of CeO2–MnO2
In order to investigate the sensitizing effect of CeO2–MnO2 nanoparticles for radiotherapy, HeLa cells were used as model cancer cells in vitro, and CeO2–MnO2 CeO2–MnO2 was co-incubated with HeLa cells to detect their cell survival rate. Figure 3A showed that the CeO2–MnO2 treatment group had toxic effects on HeLa cells. We further investigated the antitumor effect of CeO2–MnO2 combined with X-rays in vitro. Figure 3B illustrated that the combination of CeO2–MnO2 with X-rays showed enhanced cytotoxicity when compared to the X-ray group alone, as well as stronger cytotoxicity than either the CeO2 or MnO2 groups alone. By analyzing the interaction between the concentration of CeO2–MnO2 and the X-ray dose, the results were obtained by isobologram analysis. Additional file 1: Fig. S5 showed that CeO2–MnO2 has a significant radiotherapy sensitizing effect under 4 Gy. Subcellular localization experiments showed that coumarin 6-labeled CeO2–MnO2 (green fluorescence) could effectively enter HeLa cells after 4 h and that lysosomes were the main organelle targets of CeO2–MnO2 (Fig. 3F). Since radiotherapy leads to toxic effects on normal cells and tissues, the development of safe and non-toxic radiotherapy sensitizers is an urgent issue. To evaluate the radiation protection effect of CeO2–MnO2 on normal cells, we determined the cellular activity of MIHA (human normal hepatocytes) after CeO2–MnO2 combined with 4 Gy using the MTT assay. As shown in Fig. 3D, E, the cell survival rate decreased as the concentration of each drug increased in the absence of radiation irradiation. When MIHA cells were irradiated with 4 Gy, the cell survival rate in the CeO2–MnO2 group was higher than that in the X-ray alone group. However, when the concentration of CeO2–MnO2 reached 100 μg/mL, the cell survival rate decreased after the combined action with X-rays, which might be due to the toxicity of the drug dose. According to the above results, CeO2–MnO2 concentrations below 100 μg/mL may have some radiation protection effect on normal cells. CeO2–MnO2 was found to have a significant synergistic effect with X-ray in inhibiting HeLa cells using colony formation experiments (Fig. 3C). These results suggest that CeO2–MnO2 can be used as a radiation therapy sensitizer combined with X-rays to inhibit the growth of HeLa cells. High levels of ROS disrupt the intracellular redox balance and will enhance the biomolecular damage induced by ionizing radiation, which is the main mechanism by which CeO2–MnO2 enhances the effect of radiotherapy. As shown in Fig. 3G, H, CeO2–MnO2 increased the accumulation of ROS and •O2− in HeLa cells under X-ray treatment. Therefore, it can be concluded that CeO2–MnO2 may significantly enhance radiotherapy damage of HeLa cells by enhancing the production of ROS, thus exhibiting superior antitumor effects in vitro.
CeO2–MnO2 combined with X-ray regulates mitochondrial damage, cell cycle and apoptosis
Elevated ROS levels can lead to an imbalance in cellular redox homeostasis, resulting in mitochondrial dysfunction, which further induces cell damage and apoptosis. We first examined the changes in mitochondrial membrane potential (MMP, Δψm) in HeLa cells triggered by the combination of different concentrations of CeO2–MnO2 and X-rays (4 Gy) using the JC-1 probe. As shown in Fig. 4A, CeO2–MnO2 caused a slight decrease in mitochondrial membrane potential, and the change in mitochondrial membrane potential was more pronounced and concentration-dependent after combined with X-ray irradiation. This can be seen in the green fluorescence ratio (Fig. 4B). It was discovered that CeO2–MnO2 has a radiosensitizing effect. However, the percentage of apoptosis was significantly elevated after CeO2–MnO2 combined with X-ray treatment, further indicating the radiosensitizing effect of CeO2–MnO2. The percentage of apoptotic cells after treatment with different drug groups was detected using an apoptosis kit. Figure 4C showed that treatment of HeLa cells with CeO2–MnO2 and X-rays induced mainly late-stage apoptosis. The late-stage apoptosis rate increased gradually from 6.02 (control) to 30.10% after treatment with CeO2–MnO2, and further increased to 65.40% after combined with X-ray irradiation. And the effect of CeO2–MnO2 on triggering late-stage apoptosis was more significant compared to CeO2 and MnO2 alone. Furthermore, we analyzed the effect of CeO2–MnO2 combined with X-ray on the HeLa cell cycle using flow cytometry. Figure 4D shows that the group of CeO2–MnO2 combined with X-ray mainly caused elevated Sub-G1 phase in HeLa cells. These results suggest that CeO2–MnO2 can effectively enhance X-ray-induced mitochondrial damage and ultimately promote apoptosis.
Therapeutic effect of CeO2–MnO2 and MR imaging in vivo
To determine the radiosensitization effect of CeO2–MnO2 in vivo, HeLa tumor-bearing mice were divided into four groups randomly: Saline, X-ray, CeO2–MnO2, and CeO2–MnO2 + X-ray. A schematic illustration of all animal experiments is given in Fig. 5A. Due to the tumor microenvironment, CeO2–MnO2 decomposes to generate Mn2+ with T1 imaging function. Based on the above properties, we investigated the potential of CeO2–MnO2 in MRI, which can be used to assess in situ drug accumulation in tumor regions. As seen in Fig. 5B, C the T1-weighted signal intensity of tumor sites in mice was significantly enhanced at 2 h after injection, and the signal was strongest at 4 h. This indicates that CeO2–MnO2 can rapidly penetrate into the tumor and decompose in response to the tumor microenvironment, while the accumulation of CeO2–MnO2 was highest at 4 h. And with the metabolism of CeO2–MnO2, the T1 signal gradually diminished. Consequently, these results indicate that CeO2–MnO2 accumulates rapidly at tumor locations and becomes Mn2+, which can be used as a T1 contrast agent to guide tumor treatment in vivo, while this rapid metabolism also enhances biosafety. To investigate the synergistic effect between X-rays and drugs, we constructed a HeLa cell nude mouse subcutaneous tumor model. Tail vein injection of CeO2–MnO2 and X-rays synergistically kill tumor. During the treatment period, the length and width of the tumor area were measured every 2 days to calculate the tumor volume, and the weight was measured. At the end of 21 days of treatment, the CeO2–MnO2 combination radiotherapy group had a better treatment effect compared to the other groups. According to Fig. 5D, the body weight of all experimental groups did not fluctuate much during 21 days, which proved that there was no significant toxicity in CeO2–MnO2 group. Meanwhile, according to Fig. 5E, under the treatment of the CeO2–MnO2 co-X-ray, the tumor volume was the smallest after 21 days. As shown in Fig. 5F, G, the tumor mass and representative tumor photos of mice clearly showed that the anti-tumor efficiency of CeO2–MnO2 combined with X-ray treatment was superior to other treatment groups. In addition, H&E staining of tumor tissue sections showed that CeO2–MnO2 combined with radiotherapy effectively promoted apoptosis in cancer cells (Fig. 5H). To further evaluate the inhibitory effect of treatment on cancer cell proliferation and angiogenesis, immunofluorescence (IF) staining is performed using Ki67 and VEGF antibodies. Representative Ki67 and VEGF in each group are shown in Fig. 5H. At the same time, since CeO2–MnO2 catalyzes the production of O2 by H2O2, thereby improving tumor hypoxia, the enhanced synergistic therapeutic effect of X-ray and CeO2–MnO2 in overcoming tumor hypoxia is demonstrated by the expression of HIF-1α (Fig. 5H).
Biosafety of CeO2–MnO2 in vivo
We systematically evaluated the potential toxicity of CeO2–MnO2 in synergistic treatment groups with X-ray, and the microscopic images of the tissues by H&E staining showed that CeO2–MnO2 combined with X-ray had no significant toxicity to the major organs of mice (Fig. 6A). Blood was also collected to determine biochemical indexes such as ALT, AST, ALB, TP and UREA to evaluate liver, kidney and heart functions. The high safety and low toxicity of CeO2–MnO2 as a radiosensitizer in cancer treatment was confirmed compared to the healthy group (Fig. 6B). The low toxicity of the nanomedicine in vivo was confirmed, suggesting further biomedical applications of the formulation.
In conclusion, we synthesized CeO2–MnO2 nanoparticles and characterized their structures using a series of characterization tools. In addition, CeO2–MnO2 nanoparticles have anti-tumor properties and can respond to GSH and H2O2, generating large amounts of ROS and oxygen, enhancing the radiotherapy efficacy and improving the cancer microenvironment. They also have MRI functionality to pinpoint the tumor lesion at the tumor site and improve the anti-tumor effect. These properties enable CeO2–MnO2 nanoparticles to have significant anti-tumor properties in vivo and in vitro. In summary, we present a radiosensitizer that enhances the radiotherapy efficacy while ensuring low toxicity to normal sites, which will greatly help promote efficient and low toxicity radiotherapy.
Materials and methods
Cerous nitrate hexahydrate [Ce(NO3)3∙6H2O], Potassium permanganate (KMnO4),Sodium hydroxide (NaOH), Thiazolyl blue tetrazolium bromide (MTT), Propidium iodide (PI) were obtained from Sigma-Aldrich. Dulbecco’s modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were purchased from Gibco Thermofisher Scientific Inc. Hydrogen peroxide test kits was obtained from Beyotim. Annexin V-FITC/PI Apoptosis Kit was purchased from Dojindo Chemical Technology Co., Ltd (China). 30% H2O2 solutions was purchased from Guangzhou Chemical Reagent Factory (China).All animal experiments were conducted under the approval of the Animal Experimentation Ethics Committee of JinanUniversity.
The synthesis of CeO2
Ce (NO3)3∙6H2O (1.736 g) was dissolved in 10 mL ultrapure water, sodium hydroxide 19.2 g was dissolved in 70 mL of ultrapure water, and the two solutions were mixed and stirred at room temperature for 30 min. The mixture was heated to 100 °C and refluxed for 24 h. The reaction product was centrifuged at 8000 rpm for 10 min and washed three times with ultrapure water. The precipitate was dried overnight in an oven at 60 ℃. 20 mg of the dried product was dissolved in 10 mL of ultrapure water, sonicated for 2 h until completely dissolved, transferred to a Teflon bottle and calcined in an autoclave at 160 ℃ for 12 h. The product then was extracted from the reaction at 8000 rpm. After the reaction, the product was dried at 60 ℃, and the powder was obtained as CeO2.
The synthesis of MnO2
Add 20 mL of 0.1 M MnCl2 solution to 1 M NaOH to adjust pH to 10, stir vigorously at room temperature for 2 h, and dialyze the solution for 24 h to obtain MnO2 solution. Stir for 2 h. Dialyze the solution for 24 h to obtain MnO2 solution.
The synthesis of CeO2–MnO2
1.736 g of cerium nitrate hexahydrate was dissolved in 10 mL of ultrapure water and 19.2 g of sodium hydroxide was dissolved in 70 mL of ultrapure water. The two solutions were mixed at room temperature and stirred for 30 min, then the mixture was heated to 100 ℃ and refluxed for 24 h. The product was centrifuged at 8000 rpm for 10 min and washed three times with ultrapure water. The precipitate was dried overnight in an oven at 60 ℃. Add 80 mg of the product to 35 mL of KMnO4 solution at a concentration of 0.01 M. Transfer the solution to a 50 mL PTFE vial and calcine in an autoclave at 140 ℃ for 12 h. Centrifuge at 8000 rpm for 10 min and wash three times to give the final product as CeO2–MnO2.
CeO2–MnO2 catalyzes the production of O2 from H2O2 in vitro
After mixing 400 µM, 200 µM and 100 µM H2O2 with aqueous CeO2–MnO2 solution thoroughly at room temperature, the concentration of oxygen was measured using a dissolved oxygen meter and the values were recorded for 15 min.
Detection of the rate of H2O2 consumption by CeO2–MnO2 in vitro
The rate of hydrogen peroxide scavenging by CeO2, MnO2 and CeO2–MnO2 nanoparticles at 100 µg/mL was detected using the hydrogen peroxide kit, while the rate of hydrogen peroxide scavenging by CeO2–MnO2 nanoparticles at 10, 20, 40 and 80 µg/mL was detected.
GSH response of CeO2–MnO2
GSH of 8 mM, 4 mM, 2 mM, 1 mM and 0.5 mM were applied with CeO2–MnO2 for 5 min at room temperature, and then the color change of the solution was recorded and the UV–Vis absorption spectrum of the solution was detected.
Determination of cell viability
The cells involved included human cervical cancer cells, HeLa cells, and human normal hepatocytes cells, MIHA cells. HeLa cells and MIHA cells at the logarithmic growth stage were inoculated in 96-well plates at 3 × 104 cells/mL, 100 µL/well, and incubated with different concentrations of CeO2, MnO2 and CeO2–MnO2 for 8 h after 24 h. After irradiation, the cells were incubated in the incubator for 48 h. The cell survival rate was determined by MTT assay.
ROS level detection
HeLa cells at logarithmic growth stage were inoculated in 96-well plates at a density of 3 × 105 cells/mL, and incubated with the same concentration of CeO2, MnO2 and CeO2–MnO2 for 4 h. After incubation, DCFH-DA probe (Ex: 488 nm, Em: 525 nm) and DHE probe (Ex: 300 nm, Em: 610 nm) were added respectively, and incubated at 37 ℃ for half an hour, followed by exposure to 4 Gy and immediate detection of fluorescence intensity values at 5 min intervals using an enzyme marker.
Cell cycle and apoptosis assays
To demonstrate the apoptosis and cycle ratio of CeO2–MnO2 in HeLa cells, the assay was analyzed using flow cytometry. HeLa cells at logarithmic growth stage were inoculated in 6 cm dishes at a density of 8 × 104 cells/mL, and incubated with the same concentration of CeO2, MnO2 and CeO2–MnO2 for 4 h after 24 h. After exposure to 4 Gy radiation and continued incubation for 48 h, cells were collected and stained with PI for 15 min, filtered, and assayed for cell cycle. Similarly, logarithmic growth phase HeLa cells were inoculated in 6-well plates at a density of 1 × 105 cells/mL overnight, and after the cells were plastered, the same concentrations of CeO2, MnO2 and CeO2–MnO2 were added and incubated for 6 h. The cells were exposed to 4 Gy radiation and continued to be incubated for 48 h. The cells were collected and stained with PI and Annexin V for 15 min to detect the percentage of apoptosis.
Cellular localization experiments
The lysosomes and nuclei were stained and incubated with the same concentration of coumarin-6-labeled CeO2–MnO2 for 0 h, 1 h, 2 h, 4 h, 8 h and 12 h. The medium was removed and gently washed several times with PBS, and the fluorescence signal of the intracellular drug was recorded under a fluorescence microscope.
HeLa cells were inoculated in 6-well plates (2000 cells per well) and incubated in a humid CO2 incubator for 24 h. After complete cell adhesion, cells treated with 40 μg/mL of CeO2–MnO2 were co-incubated for 6 h and irradiated with X-ray radiation. 7 days later, the post-treated cells were washed with PBS, immobilized with paraformaldehyde, and then stained with 10% crystalline violet. The corresponding digital photographs were recorded and cell survival rates were calculated based on relativity analysis.
Female BALB/c-nude mice were purchased at 4 weeks of age from Beijing Vital River Laboratory Animal Technology Co., Ltd. After the quarantine period, when the mice reached 18–20 g, they were inoculated subcutaneously with 100 μL of HeLa cells at a density of 1 × 107 cells/mL. After the quarantine period, when the mice reached 18–20 g, 100 μL of HeLa cells at a density of 1 × 107 cells/mL were inoculated subcutaneously, and when the tumor volume grew to 120–150 mm3, the mice were randomly grouped to start the next step of the experiment.
Study on the antitumor activity in vivo
4 groups were randomly grouped, with 4 mice in each group (1) Blank control group: 100 µL of saline in the tail vein (2) X-ray group: 100 μL of saline in the tail vein (3) CeO2–MnO2 group: 2 mg/kg (4) CeO2–MnO2 + X-ray group: 2 mg/kg after tail vein dosing. The mice were irradiated with 4 Gy, and the total radiation dose was 40 Gy. The tumor volume was calculated by measuring the length and width of the tumor every 2 days, and the weight of the mice was recorded. 21 days later, the mice were subjected to blood sampling from the orbital plexus, and the tumor body and major organs were removed.
In vivo MR imaging
Homozygous BALB/c nude mice were injected with 10 mg/kg of CeO2–MnO2 solution in the tail and MR imaging was performed using MR imaging system.
Data are expressed as mean ± standard deviation. Calculation and analysis of all experimental results using GraphPad Prism 8.0. A two-tailed Student's t-test was applied to determine the statistical significance of the differences between the two groups, and variances between multiple groups were tested using the ANOVA. The difference from P < 0.05 (*) or P < 0.01 (**) is considered statistically significant.
Availability of data and materials
Additional file is available online.
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This work was supported by the National Natural Science Foundation of China (82102083), Guangdong Basic and Applied Basic Research Foundation (2022A1515011664), Science and Technology Plan Project of Guangzhou (202201020438), Guangdong Province Medical Research Fund (A2021033), 2020–2021 Achievement and Clinical Transformation Seedling Project of the First Affiliated Hospital of Guangzhou Medical University (ZH202108) and the Open Fund of Guangdong Provincial Key Laboratory of Functional Supramolecular Coordination Materials and Applications (2021A06).
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Additional file 1:
Figure S1. The average size of CeO2, MnO2 and CeO2–MnO2. Figure S2. Rates of hydrogen peroxide scavenging by CeO2, MnO2 and CeO2–MnO2 under 100 µg/mL. Figure S3. •O2- level of CeO2, MnO2 and CeO2–MnO2 under different X-rays (4 Gy). Figure S4. ROS level of CeO2, MnO2 and CeO2–MnO2 under different X-rays (4 Gy). Figure S5 . Isobologram analysis of the synergistic antiproliferative effect of the combined application of X-ray and CeO2–MnO2 on HeLa cells.
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Pi, F., Deng, X., Xue, Q. et al. Alleviating the hypoxic tumor microenvironment with MnO2-coated CeO2 nanoplatform for magnetic resonance imaging guided radiotherapy. J Nanobiotechnol 21, 90 (2023). https://doi.org/10.1186/s12951-023-01850-1
- Core–shell nanocomposite
- MR imaging